Cdk1 Protein-mediated Phosphorylation of Receptor-associated Protein 80 (RAP80) Serine 677 Modulates DNA Damage-induced G2/M Checkpoint and Cell Survival

Cho, Hyun Jung; Oh, Yun Jung; Han, Soon Woo; Chung, Hee Jin; Kim, Chang Hee; Lee, Nam Soo; Kim, Won-Ju; Choi, Je-Min; Kim, Hongtae

doi:10.1074/jbc.m112.401299

Cited by 28 publications

(25 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It has been reported that fenofibrate plays important role in anticancer properties via diverse mechanisms in recent years (Balfour et al, 1990;Pawlik et al, 2004;Saidi et al, 2006;Grabacka et al, 2006;Urbanska et al, 2008;Zak et al, 2010;Wilk et al, 2012;Cho et al, 2013;Zhao et al, 2013;Liang et al, 2013;Watanabe et al, 2013;Li et al, 2014). But the exact relationship between fenofibrate and irradiation has not explored.…”

Section: Jia Liu Et Almentioning

confidence: 99%

“…Cells in the G2/M were most sensitive to irradiation and most resistant during the latter part of the S phase cell cycle (Cho et al, 2013) .Therefore, it is an effective strategy to increase the sensitivity of radiotherapy through inducing G2/M arrest (Hematulin et al, 2012;Cui et al, 2012). The cell cycle involves a series of events driven by cyclins and subsequent CDKs, which further activate transcription factor proteins for cell cycle progression from one phase to another.…”

Section: Jia Liu Et Almentioning

confidence: 99%

“…In eukaryotes, the cell cycle is regulated by cyclins, cyclin-dependent kinases (CDKs) and cyclin-dependent kinase inhibitor (CDKI). In particular, cyclin B and CDK1 proteins are involved in regulating the progression of G2/M phase (Cho et al, 2013). It is widely known that cells are blocked in the G2/M phase during DNA damage, and cells are more susceptible to the cytotoxic effects of radiotherapy in the G2/M phase (Pawlik et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Fenofibrate Increases Radiosensitivity in Head and Neck Squamous Cell Carcinoma via Inducing G2/M Arrest and Apoptosis

Liu¹,

Ge²,

Zhu³

et al. 2014

Asian Pacific Journal of Cancer Prevention

View full text Add to dashboard Cite

Radiation therapy is an important treatment for head and neck squamous cell carcinoma (HNSCC). However, how to promote radiation sensitivity in HNSCC remains a challenge. This study aimed to investigate the radiosensitizing effects of fenofibrate on HNSCC and explore the underlying mechanisms. HNSCC cell lines CNE-2 and KB were subjected to ionizing radiation (IR), in the presence or absence of fenofibrate treatment. Cell growth and survival, apoptosis and cell cycle were evaluated. In addition, CNE-2 cells were xenografted into nude mice and subjected to IR and/ or fenofibrate treatment. The expression of cyclinB and CDK1 was detected by Western blotting. Our results showed that fenofibrate efficiently radiosensitized HNSCC cells and xenografts in mice, and induced apoptosis and G2/M arrest via reducing the activity of the CDK1/cyclinB1 kinase complex. These data suggest that fenofibrate could be a promising radiosensitizer for HNSCC radiotherapy.

show abstract

Section: Jia Liu Et Almentioning

confidence: 99%

Section: Jia Liu Et Almentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Fenofibrate Increases Radiosensitivity in Head and Neck Squamous Cell Carcinoma via Inducing G2/M Arrest and Apoptosis

Liu¹,

Ge²,

Zhu³

et al. 2014

Asian Pacific Journal of Cancer Prevention

View full text Add to dashboard Cite

show abstract

“…The link between oxidative stress and bad outcomes in assisted reproduction techniques is based on numerous attacks that occur in cellular metabolism during the process of replication of genetic material and in the regulation of the cell cycle. These sites of regulation, which include G1/S, intra-S and G2/M are involved in the response to DNA damage [6]. Zhang et al [7] showed that Ƴ H2AX, a marker of DNA damage, appears in H-treated sperm 2 or 2 (like OS models) and also embryos fertilized with these sperms have delay for the cleavage, which implies an arrest at the control points of the cell cycle, specifically, G2/M through ATM → Chk1 → Cdc25B/Cdc25c signaling pathway [7].…”

Section: Discussionmentioning

confidence: 99%

Seminal Volume as an Indirect Factor for Determining Success at in Vitro Fertilization

Sánchez¹

2017

OGIJ

View full text Add to dashboard Cite

The semen volume is made up by the accessory glands (90%) and germinal cells (10%) (RB Mayer, 2015). Seminal plasma has enabling factors for sperm microenvironment (Aida Pujol, 2016), allowing among many other processes, sperm maturation (Yu-Wen Kuo, 2016).Objective: To analyze the correlation of seminal volume parameters with the outcome of fertilized oocytes. Material and methods:It is a prospective observational study, including 100 couples undergoing in vitro fertilization. The seminal sample was obtained by ejaculation and analyzed with WHO's 2010 criteria (Andrology laboratory procedures manual, 2010). Data encompassed seminal volumes and fertilized oocytes. Statistical analysis was made with Kolmogorov-Smirnov normalization, determining correlation of Pearson and significance of p ≤0.05. Results:The following parameters were registered: Seminal volume: 1.5-7.6 (average 2.7 ml), number of fertilized MII oocytes: 1-33 (average 10 oocytes). Pearson correlation of 0.737 (p= 0.002). By dividing groups of fertilized oocytes and seminal volumes by standard Kolmogorov we obtained significance with the number of fertilized eggs with 4.8 and 14 oocytes (p=0.001), and we also obtained significance with the seminal volume, ranging from 2 to 3.2ml (p=0.003).Discussion: Our study shows that it exists a prognostic relationship between the seminal volume and the number of fertilized eggs in an in vitro fertilization. A seminal volume ranging from 2 to 3.2ml has a better chance to produce adequate fertilization. Volumes lower than this values may reflect inadequate sperm formation and regulation and volumes greater may reflect other inflammatory conditions, such as infection (Jared M Bienek, 2016). Conclusion:Seminal volume reflects a healthy testicular function, producing spermatozoa capable of achieving good fertilization. Alterations on the seminal volume indicate inadequate conditions for the correct formation of spermatozoa, or increased inflammation molecules that damage the microenvironment.

show abstract

“…Purification of GST-fusion protein The GST fusion protein was expressed in Escherichia coli and purified as previously described. 17 siRNA All siRNA duplexes used in this study were purchased from IDT(Integrated DNA Technologies, Inc). The sequence of NLBP siRNA1 and 2 are 5-AGAAATGAGAGATGAGCTA and AGAAGAGGTCAATGATAAA, respectively, and have been described previously.…”

Section: Plasmidsmentioning

confidence: 99%

Overexpression of a novel regulator of p120 catenin, NLBP, promotes lung adenocarcinoma proliferation

et al. 2013

View full text Add to dashboard Cite

NLBP (novel LZAP-binding protein) was recently shown to function as a tumor suppressor capable of inhibiting the NFκB signaling pathway. NLBP is also known as a negative regulator of cell invasion, and its expression is reduced in several cancer cell lines that have little invasive activity. Although these phenomena suggest that NLBP may be a potential tumor suppressor, its role as a tumor suppressor in human lung cancer is not well established. In contrast to our expectation, NLBP was highly expressed in the early stage of lung adenocarcinoma tissues, and overexpression of NLBP promoted proliferation of H1299 lung adenocarcinoma cells. We also found that p120 catenin (p120ctn) was a novel binding partner of NLBP, and that NLBP binds to the regulatory domain of p120ctn, and p120ctn associates with N-terminal region of NLBP, respectively. This binding leads to p120ctn stability to inhibit proteasomal degradation of p120ctn by inhibiting its ubiqutination. In addition, we also found that overexpression of NLBP and p120ctn in human lung cancer are closely related with adenocarcinoma compared with squamous cell carcinoma. Taken together, our findings reveal that NLBP is highly overexpressed in human lung adenocarcinoma, and that overexpression of NLBP promotes the cell proliferation of lung adenocarcinoma through interacting with p120ctn and suggest that NLBP may function as an oncogene in early stage carcinogenesis of lung adenocarcinoma.

show abstract

Cdk1 Protein-mediated Phosphorylation of Receptor-associated Protein 80 (RAP80) Serine 677 Modulates DNA Damage-induced G2/M Checkpoint and Cell Survival

Cited by 28 publications

References 24 publications

Fenofibrate Increases Radiosensitivity in Head and Neck Squamous Cell Carcinoma via Inducing G2/M Arrest and Apoptosis

Fenofibrate Increases Radiosensitivity in Head and Neck Squamous Cell Carcinoma via Inducing G2/M Arrest and Apoptosis

Seminal Volume as an Indirect Factor for Determining Success at in Vitro Fertilization

Overexpression of a novel regulator of p120 catenin, NLBP, promotes lung adenocarcinoma proliferation

Contact Info

Product

Resources

About