CDK-dependent phosphorylation of Sld2 and Sld3 initiates DNA replication in budding yeast

Tanaka, Seiji; Umemori, Toshiko; Hirai, Kazuyuki; Muramatsu, Sachiko; Kamimura, Yoichiro; Araki, Hideki

doi:10.1038/nature05465

Cited by 430 publications

(525 citation statements)

References 26 publications

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“…These observations raise additional questions about the order of assembly of additional pre-initiation factors at origins of DNA replication. In budding yeast the Rad4 TopBP1 homologue Dpb11 has been shown to interact with both Sld2 and Sld3 and those events have been shown to be CDK-dependent and similar data have recently been reported in metazoans (6,7,8,10,11,12,49). Chromatin association of Cdc45 has been previously shown to be dependent upon Mcm10, Rad4 TopBP1 , Sld3 and the GINS complex (22,23,24,47).…”

Section: ) Predictably Mcm4supporting

confidence: 61%

“…A sample of 2x10 8 cells was removed at this point (time 0) and the remaining culture returned to batch growth at 25°C. Further samples of 2x10 8 cells were taken at the indicated intervals. Cell samples were fixed and frozen as above.…”

Section: Methodsmentioning

confidence: 99%

“…In budding yeast Cdc45 and Sld3 associate with early firing origins in G1, prior to CDK and DDK activity, and with late firing origins in S phase (3,4). Evidence from budding yeast has demonstrated that Sld2 and Sld3 recruitment are pivotal events in replication initiation at origins and the two proteins are critical targets of the S phase CDK (5,6,7,8). Upon phosphorylation by CDK, Sld2 and Sld3 interact with distinct BRCT domains of a third protein, Dpb11, the Saccharomyces cerevisiae orthologue of human TopBP1, and that interaction enables recruitment to the pre-RC (6,7,8).…”

Section: Introductionmentioning

confidence: 99%

“…Evidence from budding yeast has demonstrated that Sld2 and Sld3 recruitment are pivotal events in replication initiation at origins and the two proteins are critical targets of the S phase CDK (5,6,7,8). Upon phosphorylation by CDK, Sld2 and Sld3 interact with distinct BRCT domains of a third protein, Dpb11, the Saccharomyces cerevisiae orthologue of human TopBP1, and that interaction enables recruitment to the pre-RC (6,7,8). Phosphorylation of Sld2 promotes formation of a pre-loading complex containing Dpb11, the heteromeric GINS complex, DNA Pol and Sld2 itself (9).…”

Section: Introductionmentioning

confidence: 99%

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Mcm10 interacts with Rad4/Cut5TopBP1 and its association with origins of DNA replication is dependent on Rad4/Cut5TopBP1

et al. 2011

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2Running title: Rad4 TopPB1 interacts with Mcm10 and associates with stalled replication forks. and Sld3 and to be required for the stable origin association of these two proteins. Rad4 TopBP1 chromatin association at stalled replication forks was found to be dependent upon the checkpoint protein Rad9, which was not required for Rad4 TopBP1 origin association. Comparison of the levels of chromatin association at origins of replication and stalled replication forks and the differential requirement for Rad9 suggest functional differences for Rad4 TopBP1 at these distinct sites.4

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Section: ) Predictably Mcm4supporting

confidence: 61%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Mcm10 interacts with Rad4/Cut5TopBP1 and its association with origins of DNA replication is dependent on Rad4/Cut5TopBP1

et al. 2011

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“…On the other hand, Sld2 and Sld3 are 2 critical substrates of Cdk in assembly of active initiation complex in budding yeast. 2,3 In vivo analyses of assembly of replication complex in yeast led to identification of pre-LC (pre-Landing Complex) composed of CDK-phosphorylated Sld2, Dpb11, GINS and DNA polymerase e that brings GINS to the origins. Cdc45 interacts with Sld3, which binds to origins through interaction with Mcm in a manner independent of CDK.…”

mentioning

confidence: 99%

Building up the machinery for DNA replication

Masai

2015

Cell Cycle

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Control of Mus81 nuclease during the cell cycle

Pfander

Matos

2017

FEBS Letters

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Edited by Wilhelm JustDNA replication and homologous recombination involve the formation of branched DNA structures that physically link chromosomes. Such DNAbased connections, which arise during S-phase, are typically disengaged prior to entry into mitosis, in order to ensure proper chromosome segregation. Exceptions can, however, occur: replication stress, or elevated levels of DNA damage, may cause cells to enter mitosis with unfinished replication as well as carrying recombination intermediates, such as Holliday junctions. Hence, cells are equipped with pathways that recognize and process branched DNA structures, and evolved mechanisms to enhance their function when on the verge of undergoing cell division. One of these pathways utilizes the structure-selective endonuclease Mus81, which is thought to facilitate the resolution of replication and recombination intermediates. Mus81 function is known to be enhanced upon entry into M phase in budding yeast and human cells. Based on recent findings, we discuss here an updated model of Mus81 control during the cell cycle.

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CDK-dependent phosphorylation of Sld2 and Sld3 initiates DNA replication in budding yeast

Cited by 430 publications

References 26 publications

Mcm10 interacts with Rad4/Cut5TopBP1 and its association with origins of DNA replication is dependent on Rad4/Cut5TopBP1

Mcm10 interacts with Rad4/Cut5TopBP1 and its association with origins of DNA replication is dependent on Rad4/Cut5TopBP1

Building up the machinery for DNA replication

Control of Mus81 nuclease during the cell cycle

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