CDC25 protein expression and interaction with DAZL in human corpus luteum

Pan, Hsien An; Lee, Yeu Chin; Teng, Yen-Ni; Tsai, Shaw-Jenq; Lin, Yung Ming; Kuo, Pao Lin

doi:10.1016/j.fertnstert.2008.09.025

Cited by 6 publications

(5 citation statements)

References 52 publications

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“…These findings expand our knowledge of the processes that lead to the observed inhibition of proliferation on granulosa cells during the final stages of the ovulatory process. Some of the targets were already identified in granulosa cells, including CDC25a (apoptosis, cell cycle 41 ), MSH2 (apoptosis 42 ), PRC1 (apoptosis 43 ) and STMN1 (cell migration, apoptosis 44 ). Other targets that were not previously described in granulosa cells include TOP2A (apoptosis, cell cycle), DDAH1 (apoptosis), ECT2 (cell cycle), RAD51AP1 (cell cycle), PBK (apoptosis) and NUSAP1 (cell migration, apoptosis).…”

Section: Discussionmentioning

confidence: 99%

“…This family was among the most upregulated miRNAs during final follicular maturation. Several of its negatively correlated targets were previously identified in granulosa cells, including AURKB (cell cycle 51 ), CDC25A (apoptosis, cell cycle 41 ), EZH2 (histone methylation 52 ), and THBS1 (adhesion, angiogenesis 53 ). Other targets that were not described in granulosa cells include BRCA2 (DNA repair), CDC6 (DNA replication), FANCD2 (DNA repair), MCM3 (DNA replication), PPP1R12B (myosin phosphatase), RRM2 (DNA synthesis) and CHEK1 (cell cycle).…”

Section: Discussionmentioning

confidence: 99%

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Characterization of the miRNA regulators of the human ovulatory cascade

Yerushalmi

Salmon‐Divon

Ophir

et al. 2018

Sci Rep

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Ovarian follicular development and ovulation are complex and tightly regulated processes that involve regulation by microRNAs (miRNAs). We previously identified differentially expressed mRNAs between human cumulus granulosa cells (CGCs) from immature early antral follicles (germinal vesicle - GV) and mature preovulatory follicles (metaphase II - M2). In this study, we performed an integrated analysis of the transcriptome and miRNome in CGCs obtained from the GV cumulus-oocyte complex (COC) obtained from IVM and M2 COC obtained from IVF. A total of 43 differentially expressed miRNAs were identified. Using Ingenuity IPA analysis, we identified 7288 potential miRNA-regulated target genes. Two hundred thirty-four of these target genes were also found in our previously generated ovulatory gene library while exhibiting anti-correlated expression to the identified miRNAs. IPA pathway analysis suggested that miR-21 and FOXM1 cooperatively inhibit CDC25A, TOP2A and PRC1. We identified a mechanism for the temporary inhibition of VEGF during ovulation by TGFB1, miR-16-5p and miR-34a-5p. The linkage bioinformatics analysis between the libraries of the coding genes from our preliminary study with the newly generated library of regulatory miRNAs provides us a comprehensive, integrated overview of the miRNA-mRNA co-regulatory networks that may play a key role in controlling post-transcriptomic regulation of the ovulatory process.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Characterization of the miRNA regulators of the human ovulatory cascade

Yerushalmi

Salmon‐Divon

Ophir

et al. 2018

Sci Rep

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“…In humans, mice and X. laevis DAZL expression is detectable in primordial germ cells (PGCs) and all oogenic stages examined so far (Table 1). DAZL has also been reported to be expressed in human and mouse granulosa cells (Ruggiu et al 1997, Dorfman et al 1999, human theca interna cells (Nishi et al 1999) and in the granulosa-luteal cells of human corpus lutea (Pan et al 2002(Pan et al , 2008, but this remains contraversial. In invertebrates, DAZL family protein expression is not restricted to the germ cells/gonads with Boule being expressed in Drosophila larval neurons and adult brain (Joiner & Wu 2004, Hoopfer et al 2008.…”

Section: Dazl Family Of Proteinsmentioning

confidence: 99%

The DAZL and PABP families: RNA-binding proteins with interrelated roles in translational control in oocytes

Brook¹,

Smith²,

Gray³

2009

Reproduction

111

114

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Gametogenesis is a highly complex process that requires the exquisite temporal, spatial and amplitudinal regulation of gene expression at multiple levels. Translational regulation is important in a wide variety of cell types but may be even more prevalent in germ cells, where periods of transcriptional quiescence necessitate the use of post-transcriptional mechanisms to effect changes in gene expression. Consistent with this, studies in multiple animal models have revealed an essential role for mRNA translation in the establishment and maintenance of reproductive competence. While studies in humans are less advanced, emerging evidence suggests that translational regulation plays a similarly important role in human germ cells and fertility. This review highlights specific mechanisms of translational regulation that play critical roles in oogenesis by activating subsets of mRNAs. These mRNAs are activated in a strictly determined temporal manner via elements located within their 3 0 UTR, which serve as binding sites for trans-acting factors. While we concentrate on oogenesis, these regulatory events also play important roles during spermatogenesis. In particular, we focus on the deleted in azoospermia-like (DAZL) family of proteins, recently implicated in the translational control of specific mRNAs in germ cells; their relationship with the general translation initiation factor poly(A)-binding protein (PABP) and the process of cytoplasmic mRNA polyadenylation.

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“…During meiosis, DAZL is translocated from the nucleus of the spermatogonia into the cytoplasm of secondary spermatocytes, spermatids and spermatozoa [50-53]. During oogenesis, human DAZL is expressed in the cytoplasm of oogonia and in developing follicular oocytes in fetal and adult ovaries [54-58]. Targeted deletion of the DAZL gene results in germ cell loss in both males and females in humans and mice [52].…”

Section: Introductionmentioning

confidence: 99%

Markers of stem cells in human ovarian granulosa cells: is there a clinical significance in ART?

Varras

Griva

Kalles

et al. 2012

Journal of Ovarian Research

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BackgroundThe purpose of the study was to determine the incidence of gene expression of Oct-4 and DAZL, which are typical markers for stem cells, in human granulosa cells during ovarian stimulation in women with normal FSH levels undergoing IVF or ICSI and to discover any clinical significance of such expression in ART.MethodsTwenty one women underwent ovulation induction for IVF or ICSI and ET with standard GnRH analogue-recombinant FSH protocol. Infertility causes were male and tubal factor. Cumulus–mature oocyte complexes were denuded separately and granulosa cells were analyzed for each patient separately using quantitative reverse-transcription–polymerase chain reaction analysis for Oct-4 and DAZL gene expression with G6PD gene as internal standard.ResultsG6PD and Oct-4 mRNA was detected in the granulosa cells in 47.6% (10/21). The median of Oct-4 mRNA/G6PD mRNA was 1.75 with intra-quarteral range from 0.10 to 98.21. The OCT-4 mRNA expression was statistically significantly correlated with the number of oocytes retrieved; when the Oct-4 mRNA expression was higher, then more than six oocytes were retrieved (p=0.037, Wilcoxon rank-sum). No detection of DAZL mRNA was found in granulosa cells. There was no additional statistically significant correlation between the levels of Oct-4 expression and FSH basal levels or estradiol peak levels or dosage of FSH for ovulation induction. No association was found between the presence or absence of Oct-4 mRNA expression in granulosa cells and ovarian response to gonadotropin stimulation. Also, no influence on pregnancy was observed between the presence or absence of Oct-4 mRNA expression in granulosa cells or to its expression levels accordingly.ConclusionsExpression of OCT-4 mRNA, which is a typical stem cell marker and absence of expression of DAZL mRNA, which is a typical germ cell marker, suggest that a subpopulation of luteinized granulosa cells in healthy ovarian follicles (47.6%) consists of stem cells, which are not originated from primordial germ cells. Absence of Oct-4 gene expression in more than half of the cases means probably the end of the productive journey of these cells, towards the oocyte.

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CDC25 protein expression and interaction with DAZL in human corpus luteum

Cited by 6 publications

References 52 publications

Characterization of the miRNA regulators of the human ovulatory cascade

Characterization of the miRNA regulators of the human ovulatory cascade

The DAZL and PABP families: RNA-binding proteins with interrelated roles in translational control in oocytes

Markers of stem cells in human ovarian granulosa cells: is there a clinical significance in ART?

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