CD80 and CD86 costimulatory molecules on circulating T cells of HIV infected individuals

Köchli, Christine; Wendland, Thomas; Frutig, Karin; Grunow, Roland; Merlin, Steven; Pichler, Werner J.

doi:10.1016/s0165-2478(98)00107-2

Cited by 27 publications

(20 citation statements)

References 24 publications

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“…It has been speculated that CD80 expression on human T cells in PBMC of HIV patients, or on T cells from synovial fluid of rheumatoid patients or peritoneal fluid of cancer patients, is attributable to the up-regulation of CD80 (18,19,32,37). In this study, for the first time, we demonstrated that acquisition of CD80 from APCs is indeed the mechanism for CD80 detection on T cells at the early stages of T cell activation.…”

Section: Discussionmentioning

confidence: 50%

Acquisition of CD80 by Human T Cells at Early Stages of Activation: Functional Involvement of CD80 Acquisition in T Cell to T Cell Interaction

Tatari-Calderone

Semnani

Nutman

et al. 2002

The Journal of Immunology

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The interaction between CD28 on T cells and CD80 on APCs intensifies the linkage between TCR and MHC at the site of contact between T cells and APCs. In this study, we demonstrate that during human T cell/human APC interaction, the autologous or allogeneic human CD4+ T cells become positive for the detection of CD80 at an early stage of activation (24 h). This detection of CD80 is attributable to the acquisition of CD80 from APCs, as opposed to the up-regulation of endogenous CD80, as demonstrated by CD4+ T cells treated with cyclohexamide. Furthermore, no CD80 mRNA could be detected at 24 h in T cells that had acquired CD80 from APCs. CD80 acquisition by T cells from APCs was enhanced upon TCR engagement. The amount of CD80 acquisition by CD4+ T cells was shown to be related to the expression of CD80 on APCs. Using soluble fusion proteins (soluble CTLA-4, CD28, and CD80) to block either CD28 on the surface of T cells or CD80 on the surface of APCs, it was demonstrated that CD80 acquisition by T cells is mediated through its receptors, possibly CD28 interaction. Moreover, we demonstrate that T cells that have acquired CD80 have the ability to stimulate other T cells. These data thus suggest that CD80 acquisition by human T cells might play a role in the immunoregulation of T cell responses.

show abstract

Section: Discussionmentioning

confidence: 50%

Acquisition of CD80 by Human T Cells at Early Stages of Activation: Functional Involvement of CD80 Acquisition in T Cell to T Cell Interaction

Tatari-Calderone

Semnani

Nutman

et al. 2002

The Journal of Immunology

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“…Thus, CXCR4-tropic HIV-1 Envs may use the same signaling pathway as SDF-1 to activate T cells. Circulating lymphocytes of HIV-1-infected patients exhibit increased HLA-DR and CD25 expression and decreased CD28 expression, consistent with chronic immune activation (40,52,59,74). Furthermore, levels of CD25 ϩ HLA-DR ϩ CD4 ϩ T cells correlate with disease severity (74).…”

Section: Discussionmentioning

confidence: 88%

“…HIV-1 virions were UV-inactivated for 45 min (52). Some samples were also preincubated for 1 h with 1.2 M AMD3100 (21, 80) or 10 g/ml control IgG1, anti-Fas (Calbiochem, San Diego, CA), or anti-CD86 (PharMingen).…”

mentioning

confidence: 99%

Distinct Mechanisms of CD4⁺and CD8⁺T-Cell Activation and Bystander Apoptosis Induced by Human Immunodeficiency Virus Type 1 Virions

Holm

Gabuzda

2005

J Virol

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“…Furthermore, abnormal expression of CD80 and CD86 on T cells of HIV-infected patients has been described and thought to be associated with the Department of Health and Human Services, Laboratory of Immunoregulation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892 effects of HIV-induced immune activation (28), similar to what occurs in other inflammatory diseases (29,30). Increased levels of CD80 and CD86 on B cells have also been described in inflammatory diseases such as systemic lupus erythematosus and Behçet's disease (31,32).…”

Section: H Uman Immunodeficiency Virus Infection Leads To Profound Immentioning

confidence: 99%

Deleterious Effect of HIV-1 Plasma Viremia on B Cell Costimulatory Function

Malaspina

Moir

Kottilil

et al. 2003

The Journal of Immunology

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HIV infection leads to numerous immunologic defects, including impaired B cell function. An effective humoral response requires bidirectional interactions between B cells and CD4+ T cells, critical of which are interactions between CD80/CD86 expressed on activated B cells and CD28 expressed on responder CD4+ T cells. In the present study, we examined the effect of active HIV replication on B cell costimulatory function. Induction of CD80/CD86 on B cells following B cell receptor and CD40 triggering and responsiveness of CD4+ T cells to activated B cells were investigated in a system where B cells of HIV-infected patients were compared concurrently to B cells of HIV-negative donors. In contrast to HIV-aviremic patients, B cells of HIV-viremic patients were ineffective at stimulating CD4+ T cells, as measured by the induction of activation markers and proliferation. The importance of interactions of CD80/CD86 and CD28 in activating CD4+ T cells was clear; the ablation of a normal response following the addition of neutralizing anti-CD86/CD80 Abs mirrored the response of CD4+ T cells to B cells of HIV-viremic patients, while the addition of exogenous CD28 ligands partially restored the poor CD4+ T cell response to the B cells of HIV-viremic patients. Ineffective B cell costimulatory function in HIV-viremic patients was associated with low induction of CD80/CD86 expression on B cells. Our findings further delineate the scope of defects associated with cognate B cell-CD4+ T cell interactions in HIV infection and suggest that therapeutic interventions designed to enhance CD28-dependent costimulatory pathways may help restore immune functions.

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CD80 and CD86 costimulatory molecules on circulating T cells of HIV infected individuals

Cited by 27 publications

References 24 publications

Acquisition of CD80 by Human T Cells at Early Stages of Activation: Functional Involvement of CD80 Acquisition in T Cell to T Cell Interaction

Acquisition of CD80 by Human T Cells at Early Stages of Activation: Functional Involvement of CD80 Acquisition in T Cell to T Cell Interaction

Distinct Mechanisms of CD4⁺and CD8⁺T-Cell Activation and Bystander Apoptosis Induced by Human Immunodeficiency Virus Type 1 Virions

Deleterious Effect of HIV-1 Plasma Viremia on B Cell Costimulatory Function

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CD80 and CD86 costimulatory molecules on circulating T cells of HIV infected individuals

Cited by 27 publications

References 24 publications

Acquisition of CD80 by Human T Cells at Early Stages of Activation: Functional Involvement of CD80 Acquisition in T Cell to T Cell Interaction

Acquisition of CD80 by Human T Cells at Early Stages of Activation: Functional Involvement of CD80 Acquisition in T Cell to T Cell Interaction

Distinct Mechanisms of CD4+and CD8+T-Cell Activation and Bystander Apoptosis Induced by Human Immunodeficiency Virus Type 1 Virions

Deleterious Effect of HIV-1 Plasma Viremia on B Cell Costimulatory Function

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Product

Resources

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Distinct Mechanisms of CD4⁺and CD8⁺T-Cell Activation and Bystander Apoptosis Induced by Human Immunodeficiency Virus Type 1 Virions