CD8<sup>+</sup>GITR<sup>+</sup> T cells may negatively regulate T cell overactivation in aplastic anemia

Huang, Guixuan; Zhang, Yuping; Wei, Xiaolei; Yu, Zhi; Lai, Jing; Shen, Qi; Chen, Xiaohui; Tan, Guangxiao; Chen, Cunte; Luo, Weifeng; Li, Yumiao; Zhou, Ming; Li, Yangqiu; Li, Bo

doi:10.1080/08820139.2020.1770785

Cited by 5 publications

(2 citation statements)

References 30 publications

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“…Included in these T cells are MAIT cells, which have been indicated to be involved in the immunopathology of autoimmune disease (Toubal et al, 2019). In AA, abnormal T cells mediate the immune destruction of hematopoietic stem/ progenitor cells (Huang et al, 2021;Patel et al, 2021). However, little is known about the alterations of MAIT cells and whether they play a role in the immunopathology of AA.…”

Section: Discussionmentioning

confidence: 99%

Increased IFN‐γ⁺ and TNF‐α⁺ mucosal‐associated invariant T cells in patients with aplastic anemia

Chen

Zhang

et al. 2023

Cytometry Part B Clinical

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Background Aplastic anemia (AA) is known as an autoimmune disease in which T cell activation is aberrant. It has been reported that unconventional T cells, mucosal‐associated invariant T (MAIT) cells, play an important role in several autoimmune diseases, but it is unclear if they are involved in AA. Methods In this study, we for the first time analyzed the proportions, phenotypes, and cytokine properties of MAIT cells in AA by flow cytometry. Results We found that the percentage of circulating MAIT cells was generally higher for CD3+, CD8+, and CD8− T cells in AA patients compared with healthy individuals. Moreover, the percentage of IL‐18Rα‐, NKG2D‐, IFN‐γ‐, and TNF‐α‐ positive MAIT cells was also significantly higher in AA patients. In addition, the percentage of IFN‐γ+ CD3+ or TNF‐α+CD8− MAIT cells had a significant negative correlation with the absolute neutrophil count. Conclusions We present the first observation of MAIT cells in patients with AA. MAIT cells are associated with a higher frequency of IFN‐γ and TNF‐α production and may contribute to the pathogenesis of AA.

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Section: Discussionmentioning

confidence: 99%

Increased IFN‐γ⁺ and TNF‐α⁺ mucosal‐associated invariant T cells in patients with aplastic anemia

Chen

Zhang

et al. 2023

Cytometry Part B Clinical

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“…In autoimmunity, it can be targeted to curb the over-exuberant immune response [ 20 , 39 ]. It has also been reported that the expression of CTLA-4 on Tregs in SAA was higher than that in healthy controls [ 40 – 42 ], but its expression on effector T cells is still unclear. We demonstrated that patients with AA had an increased expression of CTLA-4 on naive CD8 + T and central memory CD8 + T cells, whereas no significant difference was observed on effector T cells.…”

Section: Discussionmentioning

confidence: 99%

Mass cytometry analysis identifies T cell immune signature of aplastic anemia and predicts the response to cyclosporine

Zhang

Jin

et al. 2023

Ann Hematol

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Aplastic anemia (AA) is an auto-activated T cell–mediated bone marrow failure. Cyclosporine is often used to treat non-severe AA, which demonstrates a more heterogeneous condition than severe AA. The response rate to cyclosporine is only around 50% in non-severe AA. To better predict response to cyclosporine and pinpoint who is the appropriate candidate for cyclosporine, we performed phenotypic and functional T cell immune signature at single cell level by mass cytometry from 30 patients with non-severe AA. Unexpectedly, non-significant differences of T cell subsets were observed between AA and healthy control or cyclosporine-responder and non-responders. Interestingly, when screening the expression of co-inhibitory molecules, T cell trafficking mediators, and cytokines, we found an increase of cytotoxic T lymphocyte antigen 4 (CTLA-4) on T cells in response to cyclosporine and a lower level of CTLA-4 on CD8 + T cells was correlated to hematologic response. Moreover, a decreased expression of sphingosine-1-phosphate receptor 1 (S1P 1 ) on naive T cells and a lower level of interleukin-9 (IL-9) on T helpers also predicted a better response to cyclosporine, respectively. Therefore, the T cell immune signature, especially in CTAL-4, S1P 1 , and IL-9, has a predictive value for response to cyclosporine. Collectively, our study implies that immune signature analysis of T cell by mass cytometry is a useful tool to make a strategic decision on cyclosporine treatment of AA. Supplementary information The online version contains supplementary material available at 10.1007/s00277-023-05097-6.

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Application of serum Raman spectroscopy in rapid and early discrimination of aplastic anemia and myelodysplastic syndrome

Liang

Kong

Ren

et al. 2023

Spectrochimica Acta Part A: Molecular and Biomolecular Spectros

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CD8⁺GITR⁺ T cells may negatively regulate T cell overactivation in aplastic anemia

Cited by 5 publications

References 30 publications

Increased IFN‐γ⁺ and TNF‐α⁺ mucosal‐associated invariant T cells in patients with aplastic anemia

Increased IFN‐γ⁺ and TNF‐α⁺ mucosal‐associated invariant T cells in patients with aplastic anemia

Mass cytometry analysis identifies T cell immune signature of aplastic anemia and predicts the response to cyclosporine

Application of serum Raman spectroscopy in rapid and early discrimination of aplastic anemia and myelodysplastic syndrome

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CD8+GITR+ T cells may negatively regulate T cell overactivation in aplastic anemia

Cited by 5 publications

References 30 publications

Increased IFN‐γ+ and TNF‐α+ mucosal‐associated invariant T cells in patients with aplastic anemia

Increased IFN‐γ+ and TNF‐α+ mucosal‐associated invariant T cells in patients with aplastic anemia

Mass cytometry analysis identifies T cell immune signature of aplastic anemia and predicts the response to cyclosporine

Application of serum Raman spectroscopy in rapid and early discrimination of aplastic anemia and myelodysplastic syndrome

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Product

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CD8⁺GITR⁺ T cells may negatively regulate T cell overactivation in aplastic anemia

Increased IFN‐γ⁺ and TNF‐α⁺ mucosal‐associated invariant T cells in patients with aplastic anemia

Increased IFN‐γ⁺ and TNF‐α⁺ mucosal‐associated invariant T cells in patients with aplastic anemia