CD73-Positive Cell Spheroid Transplantation Attenuates Colonic Atrophy

Hisamatsu, Daisuke; Itakura, Natsumi; Mabuchi, Yo; Ozaki, Rion; Suto, Eriko Grace; Naraoka, Yuna; Ikeda, Akari; Ito, Lisa; Akazawa, Chihiro

doi:10.3390/pharmaceutics15030845

Cited by 2 publications

(1 citation statement)

References 64 publications

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“…These findings support the notion that DN cells possess high adipogenic differentiation potential and significant fat content in 3D culture. Hisamatsu et al found that 3D culture-derived spheroids of CD73+ cells exhibited enhanced cellular characteristics and an upregulated expression of immunomodulatory genes when compared with 2D cells [68]. In the present study, there was a tendency for lipid droplets to accumulate more in CD344-SP than in DN in 2D culture and more in DN than CD344-SP in 3D culture.…”

Section: Discussionsupporting

confidence: 53%

Quality Control of Stem Cell-Based Cultured Meat According to Specific Differentiation Abilities

Naraoka,

Mabuchi,

Kiuchi

et al. 2024

Cells

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The demand for stem cell-based cultured meat as an alternative protein source is increasing in response to global food scarcity. However, the definition of quality controls, including appropriate growth factors and cell characteristics, remains incomplete. Cluster of differentiation (CD) 29 is ubiquitously expressed in bovine muscle tissue and is a marker of progenitor cells in cultured meat. However, CD29+ cells are naturally heterogeneous, and this quality control issue must be resolved. In this study, the aim was to identify the subpopulation of the CD29+ cell population with potential utility in cultured meat production. The CD29+ cell population exhibited heterogeneity, discernible through the CD44 and CD344 markers. CD29+CD44−CD344− cells displayed the ability for long-term culture, demonstrating high adipogenic potential and substantial lipid droplet accumulation, even within 3D cultures. Conversely, CD29+CD44+ cells exhibited rapid proliferation but were not viable for prolonged culture. Using cells suitable for adipocyte and muscle differentiation, we successfully designed meat buds, especially those rich in fat. Collectively, the identification and comprehension of distinct cell populations within bovine tissues contribute to quality control predictions in meat production. They also aid in establishing a stable and reliable cultured meat production technique.

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Section: Discussionsupporting

confidence: 53%

Quality Control of Stem Cell-Based Cultured Meat According to Specific Differentiation Abilities

Naraoka,

Mabuchi,

Kiuchi

et al. 2024

Cells

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Optimization of transplantation methods using isolated mesenchymal stem/stromal cells: clinical trials of inflammatory bowel diseases as an example

Hisamatsu,

Ikeba,

Yamato

et al. 2024

Inflamm Regener

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Mesenchymal stem/stromal cells (MSCs) are distributed in various tissues and are used in clinical applications as a source of transplanted cells because of their easy harvestability. Although MSCs express numerous cell-surface antigens, single-cell analyses have revealed a highly heterogeneous cell population depending on the original tissue and donor conditions, including age and interindividual differences. This heterogeneity leads to differences in their functions, such as multipotency and immunomodulatory effects, making it challenging to effectively treat targeted diseases. The therapeutic efficacy of MSCs is controversial and depends on the implantation site. Thus, there is no established recipe for the transplantation of MSCs (including the type of disease, type of origin, method of cell culture, form of transplanted cells, and site of delivery). Our recent preclinical study identified appropriate MSCs and their suitable transplantation routes in a mouse model of inflammatory bowel disease (IBD). Three-dimensional (3D) cultures of MSCs have been demonstrated to enhance their properties and sustain engraftment at the lesion site. In this note, we explore the methods of MSC transplantation for treating IBDs, especially Crohn’s disease, from clinical trials published over the past decade. Given the functional changes in MSCs in 3D culture, we also investigate the clinical trials using 3D constructs of MSCs and explore suitable diseases that might benefit from this approach. Furthermore, we discuss the advantages of the prospective isolation of MSCs in terms of interindividual variability. This note highlights the need to define the method of MSC transplantation, including interindividual variability, the culture period, and the transplantation route.

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CD73-Positive Cell Spheroid Transplantation Attenuates Colonic Atrophy

Cited by 2 publications

References 64 publications

Quality Control of Stem Cell-Based Cultured Meat According to Specific Differentiation Abilities

Quality Control of Stem Cell-Based Cultured Meat According to Specific Differentiation Abilities

Optimization of transplantation methods using isolated mesenchymal stem/stromal cells: clinical trials of inflammatory bowel diseases as an example

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