CD56 and CD11b Positivity with Low Smac/DIABLO Expression as Predictors of Chemoresistance in Acute Myeloid Leukaemia: Flow Cytometric Analysis

Ibrahim, Abeer; Zahran, Asmaa M; Aly, Sanaa Shaker; Refaat, Ahmed; Hassan, Mohammed H.

doi:10.31557/apjcp.2018.19.11.3187

Cited by 6 publications

(12 citation statements)

References 31 publications

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“…Resistance to chemotherapy is a major obstacle in AML therapy. High ITGAM/CD56 co-expression combined with low Smac/DIABLO expression were proposed to be an important predictor of chemoresistance in AML patients [77]. The prognostic value of ITGAM in AML patients has been extensively assessed, and a correlation between high ITGAM expression and poor prognosis in AML has been established [51, 78].…”

Section: Discussionmentioning

confidence: 99%

Identification of prognostic genes in the acute myeloid leukemia microenvironment

Huang

Zhang

Fan

et al. 2019

Aging

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The tumor microenvironment (TME) has a strong influence on the progression, therapeutic response, and clinical outcome of acute myeloid leukemia (AML), one of the most common hematopoietic malignancies in adults. In this study, we identified TME-related genes associated with AML prognosis. Gene expression profiles from AML patients were downloaded from TCGA database, and immune and stromal scores were calculated using the ESTIMATE algorithm. Immune scores were correlated with clinical features such as FAB subtypes and patient’s age. After categorizing AML cases into high and low score groups, an association between several differentially expressed genes (DEGs) and overall survival was identified. Functional enrichment analysis of the DEGs showed that they were primarily enriched in the immune response, inflammatory response, and cytokine activity, and were involved in signaling processes related to hematopoietic cell lineage, B cell receptor, and chemokine pathways. Two significant modules, dominated respectively by CCR5 and ITGAM nodes, were identified from the PPI network, and 20 hub genes were extracted. A total of 112 DEGs correlated with poor overall survival of AML patients, and 11 of those genes were validated in a separate TARGET-AML cohort. By identifying TME-associated genes, our findings may lead to improved prognoses and therapies for AML.

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Section: Discussionmentioning

confidence: 99%

Identification of prognostic genes in the acute myeloid leukemia microenvironment

Huang

Zhang

Fan

et al. 2019

Aging

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“…However, mechanical hallmarks of macrophages remain independent of adhesion once differentiation was reached. CD11b has been reported as a key biomarker in immune cells for the progression of diseases like cancer and autoimmunity 25,[28][29][30][91][92][93] . Given the growing evidence that cell elasticity and fluidity suffer alterations in disease, adhesion and mechanics of immune cells may appear as relevant biomarkers 26,[94][95][96][97][98][99][100][101][102][103] .…”

Section: Discussionmentioning

confidence: 99%

“…This integrin binds to multiple extracellular matrix (ECM) and vascular proteins, like fibrinogen and ICAM-1, and is commonly used as a marker for macrophage differentiation 8,24,25 . CD11b is also known to be a therapeutic target for other diseases like Lupus, and various types of cancer and has been used as a prognosis marker in myeloid leukemia [25][26][27][28][29] . Importantly, CD11b regulates macrophage polarization 30 .…”

Section: Introductionmentioning

confidence: 99%

Coupled mechanical mapping and interference contrast microscopy reveal viscoelastic and adhesion hallmarks of monocytes differentiation into macrophages

Eroles

López-Alonso

Ortega

et al. 2022

Preprint

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Monocytes in the blood torrent, when activated by pro-inflammatory signals, adhere to the vascular endothelium and migrate into the tissue for ultimately differentiate into macrophages. Mechanics and adhesion play a crucial role in macrophage functions, such as migration and phagocytosis. However, how monocytes change their adhesion and mechanical properties upon differentiation into macrophages is still not well understood.In this work, we combined atomic force microscopy (AFM) viscoelastic mapping with interference contrast microscopy (ICM) to simultaneously probe, at the single-cell level, viscoelasticity and adhesion during monocyte differentiation. THP-1 monocytic cells were differentiated into macrophages through phorbol 12-myristate 13-acetate (PMA). Morphological quantification was achieved using holographic tomography imaging and the expression of integrin subunit CD11b was tracked as a marker of differentiation.Holographic tomography proved to be a quantitative in vivo technique, revealing a dramatic increase in macrophage volume and surface area and two subpopulations, spread and round macrophages. AFM viscoelastic mapping revealed an increased stiffness and more solid-like behavior of differentiated macrophages, especially in the lamellipodia and microvilli regions. Differentiated cells revealed an important increase of the apparent Young’s modulus (E0) and a decrease of cell fluidity (β) on differentiated cells, which correlated with an increase in adhesion area. Macrophages with a spreading phenotype enhanced these changes. Remarkably, when adhesion was eliminated, differentiated macrophages remained stiffer and more solid-like than monocytes, suggesting a permanent reorganization of the cytoskeleton. We speculate that the more solid-like microvilli and lamellipodia might help macrophages to minimize energy dissipation during mechanosensitive activity, such as phagocytosis, making it more efficient. Our proposed approach revealed viscoelastic and adhesion hallmarks of monocyte differentiation that may be important for biological function.

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“…Therefore CD11b is required for the interaction of leukemic cells in the bone marrow microenvironment. It also suppresses the immune system and is a marker for myeloidderived suppressor cells that play a role in the progression of malignancies 22,23 25 . This finding might be a clue of altered hemostasis and coagulation tests we found in CD11b positive AML patients.…”

Section: Patient Characteristicsmentioning

confidence: 99%

Akut Myeloid Lösemide CD11b İfadesinin Hemostatik Komplikasyonlar ve Tedaviye Yanıt ile İlişkisi

Baysal¹,

Ümit²,

Öztürk³

et al. 2020

Namık Kemal Tıp Dergisi

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In our study, we aimed to investigate the effects of CD11b expression on myeloblasts on clinical course and prognosis in patients with AML.Materials and Methods: Data of 123 patients diagnosed with AML between 2014-2017 in Trakya University Faculty of Medicine, Department of Hematology, a tertiary referral hospital in the Trakya Region, were evaluated in a retrospective manner. The diagnosis of AML was based on WHO 2016 criteria of Myeloid Neoplasms.Results: Of the 123 patients in our study, 60 were female, and 63 were male. The mean age was 57.93 years. CD11b positivity was observed in 40 patients. Platelet counts were significantly lower in patients with CD11b positivity (p = 0.004). Likewise, D-dimer levels at presentation were higher in the CD11b positive patient group (p = 0.000). Regarding outcomes, patients with CD11b positivity were found to have lower rates of remission with first-line remission induction therapy (p = 0.003). There was no significant relationship between CD11b positivity and overall survival with Kaplan Meier survival analysis (8.5 months in CD 11b positive group, 12.1 months in negative group, p: 0.436). Conclusion:Our study demonstrated that patients with CD11b expression had lower remission rates with remission induction chemotherapy.

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CD56 and CD11b Positivity with Low Smac/DIABLO Expression as Predictors of Chemoresistance in Acute Myeloid Leukaemia: Flow Cytometric Analysis

Cited by 6 publications

References 31 publications

Identification of prognostic genes in the acute myeloid leukemia microenvironment

Identification of prognostic genes in the acute myeloid leukemia microenvironment

Coupled mechanical mapping and interference contrast microscopy reveal viscoelastic and adhesion hallmarks of monocytes differentiation into macrophages

Akut Myeloid Lösemide CD11b İfadesinin Hemostatik Komplikasyonlar ve Tedaviye Yanıt ile İlişkisi

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