CD5 enhances Th17‐cell differentiation by regulating IFN‐γ response and RORγt localization

McGuire, Donald J.; Rowse, Amber L.; Li, Hao; Peng, Binghao J.; Sestero, Christine M.; Cashman, Kevin S.; Sarno, Patrizia De; Raman, Chander

doi:10.1002/eji.201343998

Cited by 23 publications

(29 citation statements)

References 21 publications

(41 reference statements)

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“…We found that loss of CD5-CK2 signaling had not effect on OVAp induced pS13-CDC37, the site of CK2 phosphorylation (Fig 5B). These findings suggest pAkt/pCDC37 signaling axis is not regulated via CD5-CK2 signaling in developing T lymphocytes unlike previous findings in peripheral T lymphocytes [11]. …”

Section: Resultscontrasting

confidence: 92%

“…In peripheral T cells, CD5-CK2 signaling is necessary for efficient TCR-induced activation of Akt to promote cell survival [11]. We found no difference in the activation of Akt in DP or SP thymocytes under basal conditions or following OVAp injection between CD5-WT and CD5-ΔCK2BD mice (Fig 5A).…”

Section: Resultsmentioning

confidence: 68%

“…In peripheral T cells, CD5-CK2 signaling modulates Akt activity [11], a signaling pathway down regulated during the generation of nTreg [17, 18]. We observed no difference in frequency or numbers of nTreg cells (CD4+CD25+Foxp3+) between CD5-CK2ΔBD and CD5 WT mice (Fig 3A and 3B).…”

Section: Resultsmentioning

confidence: 80%

“…Very little is known regarding the CK2 downstream effectors involved in CD5 signaling. In this context, we recently showed that peripheral T cells require CK2 binding to CD5 for optimal Akt activity [11], however these studies were not performed in thymocytes. Therefore, here we sought to determine the role of CD5-CK2 signaling during thymocyte development using a CD5 knock-in mouse in which the nucleotides corresponding to the CK2 binding domain (S458-S461) were deleted.…”

Section: Introductionmentioning

confidence: 99%

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CD5-CK2 Signaling Modulates Erk Activation and Thymocyte Survival

et al. 2016

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CD5 is well recognized for its importance in thymic selection. Although this property of CD5 has been attributed to its ITIM-domain dependent regulation of TCR-signal strength, the mechanism has not been established. A second major signaling domain within the cytoplasmic tail of CD5 is a CK2 binding/activation domain (CD5-CK2BD). Using a gene-targeted mouse in which the CD5-CK2BD is selectively ablated (CD5-ΔCK2BD), we determined that loss of function of CD5-CK2 signaling in a MHC-II selecting TCR transgenic (OT-II) mouse resulted in decrease in double positive (DP) thymocytes, which correlated with enhanced apoptosis. Remarkably, DP cells expressing high levels of CD5 and CD69 and single positive (CD4+SP) thymocytes were increased in CD5-ΔCK2BD mice indicating that CD5-CK2 signaling regulates positive selection and promotes survival. Consistent with this possibility, we determined that the activation and nuclear localization of ERK as well as apoptosis was greater in thymic populations from OTII CD5-ΔCK2BD mice than OTII CD5-WT mice following injection of OVA323-339-peptide. The mobilization of Ca2+, an early event of TCR activation, was not altered by the loss of CD5-CK2 signaling. Collectively, these data demonstrate that the CD5-CK2 signaling axis regulates positive selection by modulating activation of ERK and promoting survival independent of proximal TCR signals.

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Section: Resultscontrasting

confidence: 92%

Section: Resultsmentioning

confidence: 68%

Section: Resultsmentioning

confidence: 80%

Section: Introductionmentioning

confidence: 99%

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CD5-CK2 Signaling Modulates Erk Activation and Thymocyte Survival

et al. 2016

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“…Furthermore, considering the fact that ATRA-treated CCR6 + T cells maintain their Th17 features (42) and that mTORC1, via the induction of the kinase S6K2, is involved in the nuclear translocation of RORC (60,85), one other possibility is that mTOR inhibitors interfere with the RORC-mediated transcriptional program in Th17 cells, which is favorable to HIV replication (20). Consistent with this hypothesis, we demonstrated that decreased HIV replication in the presence of rapamycin and INK128 coincided with a significant reduction in IL-17A production by ATRA-treated CD4 + T cells.…”

Section: On Hiv Replication In Ccr6mentioning

confidence: 99%

HIV-1 selectively targets gut-homing CCR6+CD4+ T cells via mTOR-dependent mechanisms

Planas¹,

Zhang²,

Monteiro³

et al. 2017

JCI Insight

161

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Kinetics of activation marker expression after in vitro polyclonal stimulation of chicken peripheralT cells

et al. 2021

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A comprehensive analysis of T cell activation markers in chicken is lacking. Kinetics of T cell activation markers (CD25, CD28, CD5, MHC-II, CD44, and CD45) in response to in vitro stimulation of peripheral blood mononuclear cells with concanavalin A (Con A) were evaluated between two chicken lines selected for high and low levels of mannose-binding lectin in serum (L10H and L10L, respectively) by flow cytometry. L10H chickens showed a stronger response to Con A based on the frequency of T cell blasts in both the CD4 + and CD8 + compartment. The majority of the proliferating CD4 + and CD8 + T cells expressed CD25. Proliferating T cells were seen both in the CD4 + MHC-II +/− and CD8 + MHC-II +/− population. For both CD4 + and CD8 + T cells, frequencies of CD25 + and MHC-II + T cells were increased 24 h after stimulation. CD28 + frequencies were only increased on CD8 + T cells 48 h after stimulation. An increase in the relative surface expression based on mean fluorescence intensity (MFI) upon activation was observed for most markers except CD5. For CD4 + T cells, CD28 expression increased 24 h after stimulation whereas MHC-II expression increased after 48 h. For CD8 + T cells, a tendency toward an increase in CD25 expression was observed. CD28 expression started to increase 24 h after stimulation and only a transient peak in MHC-II expression on CD8 + T cells was observed after 24 h. CD44 and CD45 expressed on CD4 + and CD8 + T cells increased 24-72 h after stimulation. In summary, the frequency of CD25 + and MHC-II + T cells were shown to be early markers (24 h) for in vitro activation of both CD4 + and CD8 + T cells. Frequency of CD28 + T cells was a later marker (48 h) and only for CD8 + T cells.Surface expression of all markers (MFI) increased permanently or transiently upon activation except for CD5.

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CD5 enhances Th17‐cell differentiation by regulating IFN‐γ response and RORγt localization

Cited by 23 publications

References 21 publications

CD5-CK2 Signaling Modulates Erk Activation and Thymocyte Survival

CD5-CK2 Signaling Modulates Erk Activation and Thymocyte Survival

HIV-1 selectively targets gut-homing CCR6+CD4+ T cells via mTOR-dependent mechanisms

Kinetics of activation marker expression after in vitro polyclonal stimulation of chicken peripheralT cells

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