CD47 as a potential prognostic marker for oral leukoplakia and oral squamous cell carcinoma

Abstract

Cluster of differentiation (CD)47, which acts as a negative indicator for phagocytic cells, is overexpressed on the surface of multiple human solid tumor cell types. Avoiding phagocytosis by CD47 is required for the progression of solid tumors. The present study investigated the expression of CD47 in oral leukoplakia (OLK) and oral squamous cell carcinoma (OSCC), and preliminarily explored the impact of CD47 on the proliferation of OSCC cells. A total of 56 tissue samples, including 36 cases of OLK, 10 cases of OSCC and 10 cases of normal oral mucosa (NOM) were selected to detect the expression of CD47 by immunohistochemistry. For subgroup analysis, OLK samples were divided into OLK with low-risk dysplasia (LR-OLK) and OLK with high-risk dysplasia (HR-OLK). The subcellular localization of CD47 was determined by immunofluorescence in three OSCC cell lines (Tca8113, SCC-9 and Cal-27). The effect of CD47 antibody on the proliferation of the Cal-27 cell line was analyzed using the Cell Counting kit-8 assay. CD47 expression in OLK and OSCC lesions was significantly higher than in NOM (P<0.05). Compared with LR-OLK, the expression of CD47 in HR-OLK and OSCC cells was upregulated (P=0.0327 and P=0.0048, respectively). CD47 was highly expressed in OSCC cell lines (Tca8113, Cal-27 and SCC-9) and weakly expressed in normal oral keratinocytes. The proliferation of Cal-27 cells was inhibited by CD47 antibody in a concentration and time-dependent manner. CD47 may be a reliable biomarker for predicting the progression of oral precancer and cancer, and it may serve as an important molecular target for designing a novel therapy for oral cancer.

Inhibitory Effects of Circulating Natural Autoantibodies to CD47-derived Peptides on Oral Squamous Cell Carcinoma Cells

“…The observation was interpreted with study of apoptosis and gene expression in cells. CD47 showed the highest expression levels in all three OSCC cell lines studied, consistent with other reports that CD47 was expressed more signi cantly in oral cancer tissue than normal mucosal tissues [30]. Plentiful CD47 was expressed on the surface of three OSCC cells and targeted by anti-CD47 IgG autoantibodies.…”

Inhibitory Effects of Circulating Natural Autoantibodies to CD47-derived Peptides on Oral Squamous Cell Carcinoma Cells

“…The observation was interpreted with study of apoptosis and gene expression in cells. CD47 showed the highest expression levels in all three OSCC cell lines studied, consistent with other reports that CD47 was expressed more signi cantly in oral cancer tissue than normal mucosal tissues [30]. Plentiful CD47 was expressed on the surface of three OSCC cells and targeted by anti-CD47 IgG autoantibodies, inducing the apoptosis of these malignant cells.…”

The Depletion of the &ldquo;Don&rsquo;t-Eat-Me&rdquo; Signal CD47 Increases Radiosensitivity through Phenotypical Attenuation of Cancer Stem Cell and EMT Properties in Oral Squamous Cell Carcinoma Cells

“…of Ye X, et al showing the overexpression of CD47 in Tca8113, Cal-27 and SCC-9 OSCC cell lines and week expression of same in normal oral keratinocytes. Consistent with our datashowing that high CD47 expression confers ~20% survival disadvantage and positively correlates with disease stage progression, they also suggested that CD47 may serve as a reliable predictive biomarker for oral pre-cancer and cancer progression, thus hinting on its probable role as an important molecular target for designing novel anticancer therapeutics for OSCC patients(22). Also demonstrated to be ectopically expressed in several other cancer types, strong evidence abounds implicating CD47 in the pathogenesis and progression of malignancies by its inherent ability to inhibit the phagocytosis of transformed or malignant cells(23 -25).…”