CD44 Expression in Normal Human Skin and Skin Tumors

Yasaka, Nami; Furue, Masutaka; Tamaki, Kunihiko

doi:10.1111/j.1346-8138.1995.tb03349.x

Cited by 25 publications

(24 citation statements)

References 15 publications

(15 reference statements)

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“…These results were confirmed by Tammi et al [1]. Hyaluronan receptors have been connected with metastasis of tumour cells and the presence of CD44 was therefore studied by Ysaka et al [38] who did not find CD44 expressed on the tumour cells but in dendritic cells which could be melanocytes in the tumour islands. Similar results were reported by Baum et al [39] although when studying splice variants of CD44, they found one, which does not bind HYA, heterogeneously distributed and especially accentuated in peripheral tumour cells.…”

Section: Basal Cell Carcinomasupporting

confidence: 74%

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Hyaluronan in skin

Juhlin¹

1997

Journal of Internal Medicine

118

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Abstract. Juhlin L (University Hospital, Uppsala, Sweden). Hyaluronan in skin (Minisymposium: Hyaluronan) J Intern Med 1997; 242: 61-6.Because of the abundance of hyaluronan in skin, interest was early focused on variation in the content of the polysaccharide in various pathological conditions of this tissue. A large amount of early work utilized histological techniques of insufficient specificity but recent developments of specific analytical and staining methods for hyaluronan have supplied new data on its presence and possible role in skin disorders.

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Section: Basal Cell Carcinomasupporting

confidence: 74%

“…The expression of standard CD44 is strong as in normal skin [38,40] whilst the isoform, which does not bind HYA, is down-regulated suggesting a linkage to invasive growth.…”

Section: Squamous Cell Carcinomamentioning

confidence: 95%

Hyaluronan in skin

Juhlin¹

1997

Journal of Internal Medicine

118

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“…Basal cell and spindle cell carcinomas which infiltrate the dermis lost these variant exons partially. We did not, however, observe a downregulation of CD44s, which has been described recently for alopecia areata [44], and for basal cell carcinomas [57]. Furthermore, during revision of this manuscript, another report was published concerned on expression of CD44s, CD44v4, CD44v6 and CD44v9 in primary skin tumours and metastasis [19].…”

Section: Discussionmentioning

confidence: 55%

“…This finding is only partially in line with our observations of a selective downregulation of the epithelial isoform. The discrepancies between Yasaka et al [57] and Guttinger et al [19] may be due to the use of different antibodies and, as described above, masking of distinct epitopes by conformational changes. The notion of the partial loss of CD44v8-v10 on epidermal tumours suggest that this particular isoform may be required for the maintenance of epidermal structure and/or for integration/penetration into the coherent epidermal tissue.…”

Section: Discussionmentioning

confidence: 87%

Expression of CD44 splice variants in human skin and epidermal tumours

Seiter

Tilgen

Herrmann

et al. 1996

Vichows Archiv A Pathol Anat

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Splice variants of the adhesion molecule CD44 (CD44v) are important in the lymphatic spread of rat carcinoma cells. In several human tumours expression of CD44v correlates with tumour progression. However, little is known about the physiological functions of distinct variant exons. Here we report on the immunohistological evaluation of CD44 expression in normal human skin and epidermal tumours which do not metastasise, or do so vary rarely. Frozen tissues were stained with a panel of monoclonal antibodies, recognizing epitopes of the CD44 standard isoform, as well as of variant exons v5, v6, v7, v7-v8 and v10. Stratum basale and spinosum as well as the root shaft of hairs reacted strongly with the whole panel of anti-CD44 antibodies. Stratum corneum, acinar cells of sebaceous and eccrine sweat glands stained with anti-CD44v5, anti-CD44v6 and anti-CD44v7, but not with anti-CD44v10, the latter recognizing the "epithelial isoform" (CD44v8-v10) of CD44. Ductal cells of glands and apocrine glands did not express CD44v. Compared with its expression in normal human skin, CD44v expression was reduced in basal cell carcinoma and squamous cell carcinoma of the skin. This was particularly true of CD44v10. The expression of CD44v in normal skin and dermal appendages indicates that not all combinations of variant exons are involved in tumour progression. Since the epithelial isoform is particularly downregulated in basal cell carcinoma and squamous cell carcinoma of the skin, it is unlikely that exons v8-v10 play a role in tumour progression. Rather, they may be of functional importance in maintenance of the epidermal structure.

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“…1 and 2C, and Supplementary Fig. S4A; see also Haggerty et al, 1992;Wang et al, 1992;Yasaka et al, 1995).…”

Section: Figmentioning

confidence: 97%

Inhibition of CD44 Gene Expression in Human Skin Models, Using Self-Delivery Short Interfering RNA Administered by Dissolvable Microneedle Arrays

Lara¹,

González-González²,

Speaker³

et al. 2012

Human Gene Therapy

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Treatment of skin disorders with short interfering RNA (siRNA)-based therapeutics requires the development of effective delivery methodologies that reach target cells in affected tissues. Successful delivery of functional siRNA to the epidermis requires (1) crossing the stratum corneum, (2) transfer across the keratinocyte membrane, followed by (3) incorporation into the RNA-induced silencing complex. We have previously demonstrated that treatment with microneedle arrays loaded with self-delivery siRNA (sd-siRNA) can achieve inhibition of reporter gene expression in a transgenic mouse model. Furthermore, treatment of human cultured epidermal equivalents with sd-siRNA resulted in inhibition of target gene expression. Here, we demonstrate inhibition of CD44, a gene that is uniformly expressed throughout the epidermis, by sd-siRNA both in vitro (cultured human epidermal skin equivalents) and in vivo (full-thickness human skin equivalents xenografted on immunocompromised mice). Treatment of human skin equivalents with CD44 sd-siRNA markedly decreased CD44 mRNA levels, which led to a reduction of the target protein as confirmed by immunodetection in epidermal equivalent sections with a CD44-specific antibody. Taken together, these results demonstrate that sdsiRNA, delivered by microneedle arrays, can reduce expression of a targeted endogenous gene in a human skin xenograft model.

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CD44 Expression in Normal Human Skin and Skin Tumors

Cited by 25 publications

References 15 publications

Hyaluronan in skin

Hyaluronan in skin

Expression of CD44 splice variants in human skin and epidermal tumours

Inhibition of CD44 Gene Expression in Human Skin Models, Using Self-Delivery Short Interfering RNA Administered by Dissolvable Microneedle Arrays

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