CD44 expression in endothelial colony-forming cells regulates neurovascular trophic effect

Markers and Biomarkers of Endothelium: When Something Is Rotten in the State

“…CD44 expression in endothelial colony-forming cells (ECFCs) regulates neurovascular trophic effect [34]. ECFCs are adult endothelial progenitor cells (EPCs), which can differentiate and participate in regeneration of ECs.…”

TRPC3‐mediated Ca2+ signals as a promising strategy to boost therapeutic angiogenesis in failing hearts: The role of autologous endothelial colony forming cells

“…Non‐hematopoietic stem cells encompass the endothelial colony forming cells (ECFCs) or late outgrowth EPCs, which lack CD133 and monocytic markers, such as CD11b, CD14, and CD45, while express Flk‐1/KDR, and CXCR4, CD144, CD105, lectin UEA‐1, vWF, and are positive to AcLDL uptake (Ingram et al, ; Yoder, ). ECFCs could rescue local blood perfusion either indirectly, through the paracrine liberation of growth factors and cytokines, or directly by serving as building blocks for neovessel formation (Collett et al, ; Moccia, Zuccolo, Poletto, et al, ; Richardson & Yoder, ; Sakimoto et al, ; Yoder, ). Similar to CFU‐ECs and CACs, ECFCs may be selected from peripheral blood (PB) and UCB, although the clonogenic potential of UCB‐derived ECFCs (>70 population doublings) is remarkably higher as respect to their peripheral counterparts (20–40 population doublings) (Ingram et al, ).…”

Manufacture of endothelial colony‐forming progenitor cells from steady‐state peripheral blood leukapheresis using pooled human platelet lysate

“…25 Moreover, ECFCs promote stem cell engraftment in mice by PDGF-BB secretion 26 and secrete factors that support vascular regeneration. 27 In our study the ECFCs displayed a proangiogenic profile during their early differentiation phase with considerable production of trophic factors. They produced EGF, HGF, IL-8, MCP-1, VEGF-A, and especially PDGF-BB but not hematopoietic factors like G-CSF or stromal cell-derived factor-1.…”

“…Angiocrine factors, such as FGF‐2, VEGF‐A, and PDGF‐β are relevant mediators of EPCs', ECs', and stem cells' interplay in the vascular niche . Moreover, ECFCs promote stem cell engraftment in mice by PDGF‐BB secretion and secrete factors that support vascular regeneration . In our study the ECFCs displayed a proangiogenic profile during their early differentiation phase with considerable production of trophic factors.…”