CD44 and Its Interaction with Extracellular Matrix

Lesley, Jayne; Hyman, Robert; Kincade, Paul W.

doi:10.1016/s0065-2776(08)60537-4

Cited by 983 publications

(807 citation statements)

References 241 publications

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“…We would speculate that simultaneous differential expression of various CAMs may thus contribute to the fine modulation of intercellular interactions rather than being sine qua non to these adhesive processes, and that their dynamic change may precede changes in activation states [39], [42]important for continuing embryonic development and survival. The use of incremental CAM gene knockout animals may help to define these.…”

Section: Discussionmentioning

confidence: 99%

Regulation of cellular adhesion molecule expression in murine oocytes, peri-implantation and post-implantation embryos

Tian

O.'Neill

et al. 2002

Cell Res

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Expression of the adhesion molecules, ICAM-1, VCAM-1, NCAM, CD44, CD49d (VLA-4, α chain), and CD11a (LFA-1, α chain) on mouse oocytes, and pre-and peri-implantation stage embryos was examined by quantitative indirect immunofluorescence microscopy. ICAM-1 was most strongly expressed at the oocyte stage, gradually declining almost to undetectable levels by the expanded blastocyst stage. NCAM, also expressed maximally on the oocyte, declined to undetectable levels beyond the morula stage. On the other hand, CD44 declined from highest expression at the oocyte stage to show a second maximum at the compacted 8-cell/morula. This molecule exhibited high expression around contact areas between trophectoderm and zona pellucida during blastocyst hatching. CD49d was highly expressed in the oocyte, remained significantly expressed throughout and after blastocyst hatching was expressed on the polar trophectoderm. Like CD44, CD49d declined to undetectable levels at the blastocyst outgrowth stage. Expression of both VCAM-1 and CD11a was undetectable throughout. The diametrical temporal expression pattern of ICAM-1 and NCAM compared to CD44 and CD49d suggest that dynamic changes in expression of adhesion molecules may be important for interaction of the embryo with the maternal cellular environment as well as for continuing development and survival of the early embryo.

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Section: Discussionmentioning

confidence: 99%

Regulation of cellular adhesion molecule expression in murine oocytes, peri-implantation and post-implantation embryos

Tian

O.'Neill

et al. 2002

Cell Res

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“…At least three independent colonies from each cell type were identified that expressed a high level of v5-tagged merlin (Tr6BC1 merlin and TA3 merlin , Figure 1A-b). These cells were tested for their binding to HA, since most of the known functions of CD44 are related to its HA-binding ability, which is tightly regulated (Stamenkovic et al, 1989, Lesley et al, 1993Xu and Yu, 2003).…”

Section: Increased Expression Of Merlin Inhibits the Binding Of Fluormentioning

confidence: 99%

“…As a receptor for a major ECM component, CD44 plays an important role in regulating cell adhesion and migration (Lesley et al, 1993;Sherman et al, 1994;Stamenkovic 2000). CD44 interacts with both merlin and the ERM proteins (Tsukita et al, 1994;Hirao et al, 1996;Sainio et al, 1997;Tsukita and Yonemura, 1997).…”

Section: Increased Expression Of Merlin Inhibits the Binding Of Fluormentioning

confidence: 99%

Inhibition of the hyaluronan-CD44 interaction by merlin contributes to the tumor-suppressor activity of merlin

Bai

et al. 2006

Oncogene

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Mutation or loss of expression of merlin is responsible for neurofibromatosis type 2 (NF2), which is characterized by the development of schwannomas and other tumors of the nervous system. Like the ERM (ezrin-radixin-moesin) proteins, merlin interacts with CD44, a cell-surface receptor for hyaluronan (HA) that promotes tumorigenesis. However, the relationship between merlin and CD44 and the mechanism by which merlin exerts its tumorsuppressor function have not been elucidated. In the present study, we show that increased expression of wildtype merlin in Tr6BC1 schwannoma cells inhibits HA binding to CD44. Furthermore, we demonstrate that the residues required for this inhibitory effect and the interaction between CD44 and merlin lie within the first 50 amino acids of merlin. Overexpression of merlin inhibited subcutaneous growth of Tr6BC1 cells in immunocompromised Rag1 mice. In contrast, knocking down expression of endogenous merlin promoted tumor cell growth, as did overexpression of a merlin deletion mutant (merlinDel-1) that lacks the first 50 amino acids but not of other NH 2 -terminal deletion mutants. Together, our results demonstrate that inhibition of the CD44-HA interaction contributes to the tumor-suppressor function of merlin, and they suggest that merlin inhibits tumor growth, at least in part, by negatively regulating CD44 function.

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“…Cluster of differentiation 44 CD44 is a broadly distributed family of cell surface glycoproteins involved in Ž cell-cell and cell-matrix adhesive interactions Jalkanen et al, 1986;Stamenkovic et al, 1989;Miyake et al, 1990; . Lesley et al, 1993 . Due to alternative RNA splicing which involves at least 10 of the 19 exons, a large number Ž of CD44 isoforms are generated Screaton et al, 1992; .…”

Section: Introductionmentioning

confidence: 99%

“…Due to alternative RNA splicing which involves at least 10 of the 19 exons, a large number Ž of CD44 isoforms are generated Screaton et al, 1992; . Lesley et al, 1993 . In addition, variations in glycosylation contribute to structural and functional diversity of CD44 Ž .…”

Section: Introductionmentioning

confidence: 99%

Differential expression of CD44 splice variants in the normal human central nervous system

Kaaijk

Pals

Morsink

et al. 1997

Journal of Neuroimmunology

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General rightsIt is not permitted to download or to forward/distribute the text or part of it without the consent of the author(s) and/or copyright holder(s), other than for strictly personal, individual use, unless the work is under an open content license (like Creative Commons). Disclaimer/Complaints regulationsIf you believe that digital publication of certain material infringes any of your rights or (privacy) interests, please let the Library know, stating your reasons. In case of a legitimate complaint, the Library will make the material inaccessible and/or remove it from the website. Please Ask the Library: http://uba.uva.nl/en/contact, or a letter to: Library of the University of Amsterdam, Secretariat, Singel 425, 1012 WP Amsterdam, The Netherlands. You will be contacted as soon as possible. Abstract Ž . Cluster of differentiation 44 CD44 is a broadly distributed group of glycoproteins that are involved in many functions related to Ž . cell-cell and cell-matrix interactions. In the present study, the expression of the standard form of CD44 CD44s and of CD44 variants Ž . CD44v was explored immunohistochemically on frozen sections of various areas of the human CNS. The results demonstrate that CD44s epitopes are expressed predominantly by white matter astrocytes, whereas different CD44 variant molecules are present in neurons, on axonal membranes, on endothelium or on choroid plexus epithelium. Interestingly, neurons and axons differentially expressed CD44 variant epitopes but consistently lack immunoreactivity for CD44s epitopes. Another interesting finding was that some CD44 variant epitopes expressed by neurons were localized in the cytoplasm instead of on the cell membrane. The broad distribution of variant CD44 molecules in the human CNS suggests that CD44 may play an important role in many biological processes in the CNS.

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CD44 and Its Interaction with Extracellular Matrix

Cited by 983 publications

References 241 publications

Regulation of cellular adhesion molecule expression in murine oocytes, peri-implantation and post-implantation embryos

Regulation of cellular adhesion molecule expression in murine oocytes, peri-implantation and post-implantation embryos

Inhibition of the hyaluronan-CD44 interaction by merlin contributes to the tumor-suppressor activity of merlin

Differential expression of CD44 splice variants in the normal human central nervous system

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