CD44 — A molecule involved in leukocyte adherence and T-cell activation

Haynes, Barton F.; Telen, Marilyn J.; Hale, Laura P.; Denning, Stephen M.

doi:10.1016/0167-5699(89)90040-6

Cited by 507 publications

(229 citation statements)

References 58 publications

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“…Such cells are found mainly in the medulla of the thymus (26). Thus it appears that development of Pgp-1 hiBh expressing thymocytes may be controlled by interaction with MHC class II molecules.…”

Section: Discussionmentioning

confidence: 93%

“…CD4+CD8 1 "* cells in both normaJ and mutant mice were mostty TCR^ expressing thymocytes. Expression of the Pgp-1 antigen on the cell surface of thymocytes changes during development and was found to be highest on medullary thymocytes (26). Cytofluorometric analysis revealed that Pgp-1 hl°h expressing cells were diminished by a factor of -2 in B6-Aa°/Aa° mutants (not shown).…”

Section: Phenotype Of Thymocytesmentioning

confidence: 99%

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Targeted disruption of the MHC class II Aa gene in C57BL/6 mice

et al. 1993

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The MHC class II gene Aa was disrupted by targeted mutation In embryonic stem (ES) cells derived from C57BL/6 mice to prevent expression of MHC class II molecules. Contrary to previous reports, the effect of the null-mutation on T cell development was Investigated In C57BL/6 mice, which provide a defined genetic background. The complete lack of cell surface expression of MHC class II molecules In B6-Aa°IAa° homozygous mutant mice was directly demonstrated by cytofluorometrlc analysis using antl-A b and anti-la specific mAbs. Development of CD4 + CD8" T cells In the thymus was largely absent except for a small population of thymocytes expressing high levels of CD4 together with low amounts of CDS. The majority of these cells express the TCR at high density. Although mature CD4+CD8~ T cells were undetectable In the thymus, some T cells with a CD4 + CD8~TCR hlBh phenotype were found In lymph nodes and spleen. Peripheral T cells from the mutant mice can be polyclonally activated In vitro with the mltogen concanavalln A. However, they could not be stimulated with staphylococcal enterotoxln B In autologous lymphocyte reactions, thereby demonstrating the absence of MHC class II expression in these mice. Peripheral B cells In B6-A8°/Aa° mutants were functional and responded to the T cell independent antigen levan by the production of antigenspecific IgM antibodies similar to wild-type cells. The B6-Aa°IAa° mutant mice described In this study represent an important tool to Investigate the Involvement of MHC class II molecules in lymphocyte maturation and the Immune response.

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Section: Discussionmentioning

confidence: 93%

Section: Phenotype Of Thymocytesmentioning

confidence: 99%

Targeted disruption of the MHC class II Aa gene in C57BL/6 mice

et al. 1993

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“…CD31 is found on endothelial cells, platelets, and leukocytes and has previously been implicated in endothelial cell adhesion as well as platelet-monocyte adhesion (5). CD44 is a 90-kd transmembrane protein with some homology to cartilage link proteins that exists on many cell types and may function in cell-substratum interactions (6). L-selectin is a 90-kd member of the selectin supergene family.…”

Section: Discussionmentioning

confidence: 99%

Adhesion molecule expression in human synovial tissue

Johnson

Haines

Harlow

et al. 1993

Arthritis & Rheumatism

206

130

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Objective. We have previously shown that E‐selectin is expressed on endothelium in rheumatoid arthritis (RA) synovial tissues, and hence may be important in recruitment of leukocytes into the inflamed joint. In the present study, we determined whether other cellular adhesion molecules, including selectins and members of the integrin and immunoglobulin supergene families, are expressed in frozen synovium. Methods. We employed immunohistochemical staining to determine the distribution of CD31 (PECAM), CD44 (hyaluronate receptor), CD62 (P‐selectin), Leu‐8 (L‐selectin), and the integrin subunits α5 (VLA‐5), α6 (VLA‐6), β1 (VLA 1–6), and β3 (vitronectin receptor), in synovial tissue from 9 RA and 9 osteoarthritis (OA) patients, and from 3 normal (NL) subjects. Results. P‐selectin was expressed on vascular endothelium in all synovial tissues examined. L‐selectin and α5‐integrin, while expressed on a variety of cell types, were not differentially expressed on RA synovial tissues. Integrin subunits α6 and β1 were down‐regulated on some RA synovial tissue components. In contrast, CD31 was expressed to a greater extent on RA than on OA lining cells and macrophages (P < 0.05). CD44 was expressed to a greater extent on RA or OA macrophages, lining cells, and fibroblasts compared with NL (P < 0.05). Integrin subunit β3 was strongly expressed on RA synovial blood vessels compared with NL (P < 0.05). Conclusion. The expression of integrins VLA 1–6, and selectins P and L is not up‐regulated in RA synovial tissues. CD31 and CD44 are up‐regulated on RA macrophages and lining cells, CD44 on RA fibroblasts, and β3‐integrin on RA blood vessels. The up‐regulation of CD31, CD44, and β3‐integrin in RA synovial tissues may help tip the balance of adhesive interactions toward passage and retention of leukocytes in the inflamed joint.

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“…CD44 is a broadly distributed family of cell surface glycoproteins that are now generally believed to be cell adhesion molecules with proposed functions in extracellular matrix binding, cell migration, lymphopoiesis, and lymphocyte homing (1)(2)(3). Previous studies have concluded that CD44 represents a single gene, located on the short arm of chromosome 11 in humans (4).…”

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confidence: 99%

CD44H and CD44V6 Expression in Different Subtypes of Hodgkin Lymphoma

Anwar

Wood

2000

Modern Pathology

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CD44 is a broadly distributed family of cell surface glycoproteins. The expression of CD44H has been documented in both Hodgkin lymphoma and nonHodgkin lymphoma. CD44V6 has been associated with more aggressive behavior in non-Hodgkin lymphoma, but such a correlation has not been established in Hodgkin lymphoma. In addition, the utility of CD44 and CD44V6 in the subclassification of Hodgkin lymphoma in paraffin-embedded tissues has not previously been evaluated. The current study included formalin-or methacarn-fixed, paraffin-embedded tissue specimens from 42 patients with Hodgkin lymphoma (25 nodular sclerosis, three interfollicular, four lymphocyte-rich classic Hodgkin, six lymphocyte predominant, and four mixed cellularity). The clinical stage of the study population at initial presentation ranged from stage IA to IVB. Evaluation of CD44H and CD44V6 (Novocastra) was performed by ABC immunoperoxidase technique after heat-induced epitope retrieval. In the six cases of lymphocyte predominant Hodgkin, the neoplastic cells lacked reactivity with CD44H reminiscent of their normal germinal center counterparts. On the other hand, classic Hodgkin lymphoma showed variable membranous and Golgi reactivity in the neoplastic cells in all cases irrespective of disease stage at presentation. In all cases, the neoplastic cells lacked reactivity with CD44V6 except for three (one lymphocyte predominant, one interfollicular, and one nodular sclerosis), all of which represented recurrent cases. In conclusion, CD44 evaluation is useful in the distinction between lymphocyte predominant and classic Hodgkin lymphoma. The presence of CD44H expression has no relation to the clinical stage of the disease at presentation or recurrence. CD44V6 is detected in a minority of cases irrespective of the histologic subtype and its presence may be associated with recurrence. There was no correlation between disease stage at presentation and the expression of CD44V6.KEY WORDS: CD44, CD44V6, Hodgkin lymphoma, Immunohistochemistry, Paraffin.Mod Pathol 2000;13(10)

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CD44 — A molecule involved in leukocyte adherence and T-cell activation

Cited by 507 publications

References 58 publications

Targeted disruption of the MHC class II Aa gene in C57BL/6 mice

Targeted disruption of the MHC class II Aa gene in C57BL/6 mice

Adhesion molecule expression in human synovial tissue

CD44H and CD44V6 Expression in Different Subtypes of Hodgkin Lymphoma

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