Cd43

Torres-Huerta, Alvaro; Alemán-Navarro, Estefanía; Bravo-Adame, Maria Elena; Sandoval-Hernández, Monserrat Alba; Migueles-Lozano, Oscar Arturo; Rosenstein, Yvonne

doi:10.1007/978-3-319-67199-4_523

Cited by 3 publications

(4 citation statements)

References 49 publications

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“…Differences in the protrusion of these receptors from the cell surface should impact how accessible they are to their ligands. The immune cell glycocalyx is dominated by CD43 and CD45, which extend up to 45 and 51 nm from the cell surface, respectively [68][69][70] . With a persistence length of ~41.5 nm, TIM-4 could span much of this distance, bringing its IgV domain close to the external environment.…”

Section: Functional Consequences Of Tim Protein Mucin Domainsmentioning

confidence: 99%

Glycoproteomic landscape and structural dynamics of TIM family immune checkpoints enabled by mucinase SmE

Chongsaritsinsuk,

Steigmeyer,

Mahoney

et al. 2023

Nat Commun

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Mucin-domain glycoproteins are densely O-glycosylated and play critical roles in a host of biological functions. In particular, the T cell immunoglobulin and mucin-domain containing family of proteins (TIM-1, -3, -4) decorate immune cells and act as key regulators in cellular immunity. However, their dense O-glycosylation remains enigmatic, primarily due to the challenges associated with studying mucin domains. Here, we demonstrate that the mucinase SmE has a unique ability to cleave at residues bearing very complex glycans. SmE enables improved mass spectrometric analysis of several mucins, including the entire TIM family. With this information in-hand, we perform molecular dynamics (MD) simulations of TIM-3 and -4 to understand how glycosylation affects structural features of these proteins. Finally, we use these models to investigate the functional relevance of glycosylation for TIM-3 function and ligand binding. Overall, we present a powerful workflow to better understand the detailed molecular structures and functions of the mucinome.

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Section: Functional Consequences Of Tim Protein Mucin Domainsmentioning

confidence: 99%

Glycoproteomic landscape and structural dynamics of TIM family immune checkpoints enabled by mucinase SmE

Chongsaritsinsuk,

Steigmeyer,

Mahoney

et al. 2023

Nat Commun

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“…Likewise, ECM remodeling fosters stromal cells and tumor cells to release growth factors that contribute to the development of new blood vessels, favoring tumor cell proliferation, survival, migration, and the colonization of new niches (Yadav et al, 2015). CD43 (sialophorin, SPN), a Type I protein that participates in cell-cell adhesion, intracellular signaling, migration, proliferation, and apoptosis (Torres-Huerta et al, 2018) has long been considered to be a leukocyte-specific molecule. However, a growing number of publications report the aberrant expression of CD43 in nonhematopoietic tumors.…”

Section: Introductionmentioning

confidence: 99%

“…CD43 (sialophorin, SPN), a Type I protein that participates in cell–cell adhesion, intracellular signaling, migration, proliferation, and apoptosis (Torres‐Huerta et al, 2018) has long been considered to be a leukocyte‐specific molecule. However, a growing number of publications report the aberrant expression of CD43 in nonhematopoietic tumors.…”

Section: Introductionmentioning

confidence: 99%

CD43 (sialophorin) is involved in the induction of extracellular matrix remodeling and angiogenesis by lung cancer cells

Vega-Mendoza

Cañas-Linares

Flores-Alcantar

et al. 2021

Journal Cellular Physiology

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Aberrant expression of CD43 in malignant tumors of nonhematopoietic origin such as those from lung, cervix, colon, and breast has been shown to correlate with poor prognosis, providing tumor cells with enhanced motility, anchorage-independent growth, and in vivo tumor size, while protecting the cells of NK lysis and apoptosis. To further characterize the role of CD43 in cell transformation, we tested whether interfering its expression modified the capacity of the A549 non-small cell lung cancer cells to secrete molecules contributing to malignancy. The proteomic analysis of the secretome of serumstarved A549 cells revealed that cells expressing normal levels of CD43 released significantly high levels of molecules involved in extracellular matrix organization, angiogenesis, platelet degranulation, collagen degradation, and inflammation, as compared to CD43 RNAi cells. This data reveals a novel and unexpected role for CD43 in lung cancer development, mainly in remodeling the tumor microenvironment.

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“…Its rigid rod-like shape extending 45 nm from the cell surface [ 10 ] suggests that it may be involved in the initial contact between cells through its ligands ICAM-1, Siglec-1 and -7, MHC-I, and E-selectin [ 11 , 12 , 13 , 14 , 15 , 16 ]. Due to its high sialic acid content, it is considered an anti-adhesive molecule, but when interacting with one of its ligands, it functions as a co-receptor molecule that generates intracellular signals that regulate leukocyte activation and migration [ 17 , 18 ]. CD43 alone has been shown to induce gene expression in the absence of additional signals provided by other molecules [ 19 ].…”

Section: Introductionmentioning

confidence: 99%

Differential Impact of CD43 and CD28 on T-Cell Differentiation Depending on the Order of Engagement with the TCR

Sandoval-Hernández,

Fierro,

Veytia-Bucheli

et al. 2024

IJMS

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The combination of signals from the T-cell receptor (TCR) and co-stimulatory molecules triggers transcriptional programs that lead to proliferation, cytokine secretion, and effector functions. We compared the impact of engaging the TCR with CD28 and/or CD43 at different time points relative to TCR engagement on T-cell function. TCR and CD43 simultaneous engagement resulted in higher CD69 and PD-1 expression levels than in TCR and CD28-stimulated cells, with a cytokine signature of mostly effector, inflammatory, and regulatory cytokines, while TCR and CD28-activated cells secreted all categories of cytokines, including stimulatory cytokines. Furthermore, the timing of CD43 engagement relative to TCR ligation, and to a lesser degree that of CD28, resulted in distinct patterns of expression of cytokines, chemokines, and growth factors. Complete cell activation was observed when CD28 or CD43 were engaged simultaneously with or before the TCR, but ligating the TCR before CD43 or CD28 failed to complete a cell activation program regarding cytokine secretion. As the order in which CD43 or CD28 and the TCR were engaged resulted in different combinations of cytokines that shape distinct T-cell immune programs, we analyzed their upstream sequences to assess whether the combinations of cytokines were associated with different sets of regulatory elements. We found that the order in which the TCR and CD28 or CD43 are engaged predicts the recruitment of specific sets of chromatin remodelers and TFSS, which ultimately regulate T-cell polarization and plasticity. Our data underscore that the combination of co-stimulatory molecules and the time when they are engaged relative to the TCR can change the cell differentiation program.

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Cd43

Cited by 3 publications

References 49 publications

Glycoproteomic landscape and structural dynamics of TIM family immune checkpoints enabled by mucinase SmE

Glycoproteomic landscape and structural dynamics of TIM family immune checkpoints enabled by mucinase SmE

CD43 (sialophorin) is involved in the induction of extracellular matrix remodeling and angiogenesis by lung cancer cells

Differential Impact of CD43 and CD28 on T-Cell Differentiation Depending on the Order of Engagement with the TCR

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