CD40L-expressing CD4+ T cells prime adipose-derived stromal cells to produce inflammatory chemokines

Dulong, Joëlle; Loisel, Séverine; Rossille, Delphine; Léonard, Simon; Bescher, Nadège; Bézier, Isabelle; Latour, Maëlle; Monvoisin, Céline; Monnier, Delphine; Bertheuil, Nicolas; Roulois, David; Tarte, Karin

doi:10.1016/j.jcyt.2022.01.006

Cited by 7 publications

(2 citation statements)

References 39 publications

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“…Recently, it has been reported that stimulated CD4 T cells can upregulate CD40 on ASCs and trigger IL-8 production by ASCs in a CD40 ligand-dependent manner, enabling ASCs to perform crosstalk with immune cells in an inflammatory context. 83 …”

Section: Discussionmentioning

confidence: 99%

Immunomodulatory Functions of Adipose Mesenchymal Stromal/Stem Cell Derived From Donors With Type 2 Diabetes and Obesity on CD4 T Cells

et al. 2023

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For adipose stromal/stem cell (ASCs)-based immunomodulatory therapies, it is important to study how donor characteristics, such as obesity and type 2 diabetes (T2D), influence ASCs efficacy. Here, ASCs were obtained from two groups, donors and T2D and obesity (dASCs) or nondiabetic donors with normal-weight (ndASCs), and then cultured with anti-CD3/CD28-stimulated allogeneic CD4 T cells. ASCs were studied for the expression of the immunomodulators CD54, CD274, and indoleamine 2, 3 dioxygenase 1 (IDO) in inflammatory conditions. CD4 T cells cultured alone or in cocultures were assessed to evaluate proliferation, activation marker surface expression, apoptosis, the regulatory T cells (Tregs; CD4 + CD25 high FOXP3 +) frequency, and intracellular cytokine expression using flow cytometry. Modulation of T-cell subset cytokines was explored via ELISA. In inflammatory conditions, the expression of CD54, CD274 and IDO was significantly upregulated in ASCs, with no significant differences between ndASCs and dASCs. dASCs retained the potential to significantly suppress CD4 T-cell proliferation, with a slightly weaker inhibitory effect than ndASCs, which was associated with significantly reduced abilities to decrease IL-2 production and increase IL-8 levels in cocultures. Such attenuated potentials were significantly correlated with increasing body mass index. dASCs and ndASCs comparably reduced CD4 T-cell viability, HLA-DR expression, and interferon-gamma production and conversely increased CD69 expression, the Tregs percentage, and IL-17A production. Considerable amounts of the immunomodulators prostaglandin E2 (PGE2) and IL-6 were detected in the conditioned medium of cocultures. These findings suggest that ASCs obtained from donors with T2D and obesity are receptive to the inflammatory environment and able to modulate CD4 T cells accordingly.

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Section: Discussionmentioning

confidence: 99%

Immunomodulatory Functions of Adipose Mesenchymal Stromal/Stem Cell Derived From Donors With Type 2 Diabetes and Obesity on CD4 T Cells

et al. 2023

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“…First, cellular composition is often deciphered using flow cytometry, which uses specific markers to quantify cell types, such as ASCs (CD34+, CD31−, CD45−), endothelial cells (CD31+), and immune cells (CD45+). Additionally, microscopy, such as histological or fluorescent examinations, visually presents cellular composition [3,4,[27][28][29][30][31][32][33][34] (Table 2).…”

Section: Discussionmentioning

confidence: 99%

Analyzing the Clinical Potential of Stromal Vascular Fraction: A Comprehensive Literature Review

Goncharov,

Koval,

Igorevich

et al. 2024

Medicina

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Background: Regenerative medicine is evolving with discoveries like the stromal vascular fraction (SVF), a diverse cell group from adipose tissue with therapeutic promise. Originating from fat cell metabolism studies in the 1960s, SVF’s versatility was recognized after demonstrating multipotency. Comprising of cells like pericytes, smooth muscle cells, and, notably, adipose-derived stem cells (ADSCs), SVF offers tissue regeneration and repair through the differentiation and secretion of growth factors. Its therapeutic efficacy is due to these cells’ synergistic action, prompting extensive research. Methods: This review analyzed the relevant literature on SVF, covering its composition, action mechanisms, clinical applications, and future directions. An extensive literature search from January 2018 to June 2023 was conducted across databases like PubMed, Embase, etc., using specific keywords. Results: The systematic literature search yielded a total of 473 articles. Sixteen articles met the inclusion criteria and were included in the review. This rigorous methodology provides a framework for a thorough and systematic analysis of the existing literature on SVF, offering robust insights into the potential of this important cell population in regenerative medicine. Conclusions: Our review reveals the potential of SVF, a heterogeneous cell mixture, as a powerful tool in regenerative medicine. SVF has demonstrated therapeutic efficacy and safety across disciplines, improving pain, tissue regeneration, graft survival, and wound healing while exhibiting immunomodulatory and anti-inflammatory properties.

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Bariatric Surgery Improves Serum CD40L Levels as a Predictor of Cardiovascular Risk: Systematic Review and Meta-analysis

Jamialahamdi,

Eid,

Nguyen

et al. 2024

OBES SURG

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CD40L-expressing CD4+ T cells prime adipose-derived stromal cells to produce inflammatory chemokines

Cited by 7 publications

References 39 publications

Immunomodulatory Functions of Adipose Mesenchymal Stromal/Stem Cell Derived From Donors With Type 2 Diabetes and Obesity on CD4 T Cells

Immunomodulatory Functions of Adipose Mesenchymal Stromal/Stem Cell Derived From Donors With Type 2 Diabetes and Obesity on CD4 T Cells

Analyzing the Clinical Potential of Stromal Vascular Fraction: A Comprehensive Literature Review

Bariatric Surgery Improves Serum CD40L Levels as a Predictor of Cardiovascular Risk: Systematic Review and Meta-analysis

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