CD40 signaling induces reciprocal outcomes in Leishmania-infected macrophages; roles of host genotype and cytokine milieu

Nunes, Marise P.; Cysne-Finkelstein, Léa; Monteiro, Bruna C.; Souza, Daniel M.; Gomes, Nitza A.; DosReis, George A.

doi:10.1016/j.micinf.2004.08.022

Cited by 15 publications

(11 citation statements)

References 35 publications

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“…To assess the activity of the plant aqueous extracts against the amastigote stage of the parasites, we utilized a model of infection on a cover glass [22]. The murine macrophages (the J774 cell line) were prepared in 24-well vessels (Corning) at 2 × 10 5 adherent cells/well and infected with 2 × 10 6 promastigotes in glass coverslips, inside which was placed 1 mL of culture medium.…”

Section: Methodsmentioning

confidence: 99%

Antileishmanial Activity of Medicinal Plants Used in Endemic Areas in Northeastern Brazil

Queiroz

Dias

Matta

et al. 2014

Evidence-Based Complementary and Alternative Medicine

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This study investigates the leishmanicidal activity of five species of plants used in folk medicine in endemic areas of the state of Alagoas, Brazil. Data were collected in the cities of Colonia Leopoldina, Novo Lino, and União dos Palmares, Alagoas state, from patients with cutaneous leishmaniasis (Leishmania amazonensis) who use medicinal plants to treat this disease. Plants extracts were tested at a concentration of 1–100 μg/mL in all experiments, except in an assay to evaluate activity against amastigotes, when 10 μg/mL was used. All plants extracts did not show deleterious activity to the host cell evidenced by LDH assay at 100, 10, and 1 μg/mL after 48 h of incubation. The plants extracts Hyptis pectinata (L.) Poit, Aloe vera L., Ruta graveolens L., Pfaffia glomerata (Spreng.) Pedersen, and Chenopodium ambrosioides L. exhibited direct activity against extracellular forms at 100 μg/mL; these extracts inhibited growth by 81.9%, 82.9%, 74.4%, 88.7%, and 87.4%, respectively, when compared with promastigotes. The plants extracts H. pectinata, A. vera, and R. graveolens also significantly diminished the number of amastigotes at 10 μg/mL, inhibiting growth by 85.0%, 40.4%, 94.2%, and 97.4%, respectively, when compared with control. Based on these data, we conclude that the five plants exhibited considerable leishmanicidal activity.

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Section: Methodsmentioning

confidence: 99%

Antileishmanial Activity of Medicinal Plants Used in Endemic Areas in Northeastern Brazil

Queiroz

Dias

Matta

et al. 2014

Evidence-Based Complementary and Alternative Medicine

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“…The effects of Leishmania on CD40 signaling vary in macrophages from resistant (C57BL/6) versus susceptible (BALB/c) mice [115]. While CD40 stimulation induced anti- L. major activity in macrophages from C57BL/6 mice, macrophages from BALB/c exhibited increased parasite replication [115].…”

Section: Leishmaniamentioning

confidence: 99%

“…While CD40 stimulation induced anti- L. major activity in macrophages from C57BL/6 mice, macrophages from BALB/c exhibited increased parasite replication [115]. This discrepant outcome appears to be explained by the induction of IL-10 in CD40-stimulated macrophages from BALB/c mice [115]. Relevant to these findings, L. major has been reported to skew intracellular signaling in CD40-stimulated macrophages from BALB/c mice [47].…”

Section: Leishmaniamentioning

confidence: 99%

CD40 and the immune response to parasitic infections

Subauste

2009

Seminars in Immunology

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The interaction between CD40 and CD154 regulates many aspects of cellular and humoral immunity. The CD40 -CD154 pathway is important for resistance against a variety of parasites. Studies done with these pathogens have provided important insight into the various mechanisms by which this pathway enhances host protection, mechanisms by which pathogens subvert CD40 signaling, conditions in which the CD40 -CD154 pathway promotes disease and on modulation of this pathway for immunotherapy.

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“…This observation may be explained by the fact that prolactin/receptor complex up‐regulates the release of endogenous TNF‐ α , IL‐6 and IL‐1 β , which could trigger anti‐parasitic activity (Oswald et al. 1992; Nunes et al. 2005).…”

Section: Discussionmentioning

confidence: 99%

Hypothalamic‐pituitary gonadal axis and immune response imbalance during chronic filarial infections

Mavoungou¹,

Poaty‐Mavoungou

Ongali³

et al. 2005

Tropical Med Int Health

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SummaryBi-directional relationships operate between the hypothalamic-pituitary-gonadal axis and the immune system. Cytokines, peptide hormones and their shared receptors/ligands are used as a common biological language for communication within and between the immune and neuroendocrine systems. Such communication suggests an immunoregulatory role for the brain and a sensory function for the immune system. We used a radioimmunoassay to measure the concentrations of steroid hormones (cortisol, testosterone, estradiol and progesterone) and pituitary hormones [follicle stimulating hormone (FSH), luteinizing hormone (LH) human chorionic gonadotropin (HCG) and prolactin] in peripheral blood plasma from 78 young Gabonese women with chronic filarial infections. We used an enzyme-linked immunosorbent assay to determine the concentrations of four proinflammatory cytokines [tumor necrosis factor-alpha (TNF-a), gamma interferon (IFN-c), interleukin-1 (IL-1) and IL-6] in the same plasma samples. Progesterone was unchanged and all other steroid hormone plasma concentrations were lower in microfilaremic women than in amicrofilaremic women. The concentration of LH was higher in amicrofilaremic women, whereas the prolactin concentration was higher in microfilaremics. The plasma concentrations of TNF-a, IFN-c, IL-1 and IL-6 were higher in microfilaremic women. A strong negative correlation was found between the steroid and pituitary hormones and the pro-inflammatory cytokines. Conversely, a strong positive correlation was found between prolactin and the same cytokines. These data provide first evidence of immune system and hormonal system disturbance during chronic filarial infections and suggest that the observed imbalance should be taken into account in the diagnosis and treatment of filarial infections.

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CD40 signaling induces reciprocal outcomes in Leishmania-infected macrophages; roles of host genotype and cytokine milieu

Cited by 15 publications

References 35 publications

Antileishmanial Activity of Medicinal Plants Used in Endemic Areas in Northeastern Brazil

Antileishmanial Activity of Medicinal Plants Used in Endemic Areas in Northeastern Brazil

CD40 and the immune response to parasitic infections

Hypothalamic‐pituitary gonadal axis and immune response imbalance during chronic filarial infections

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