CD40 ligand supports the long-term maintenance and differentiation of chicken B cells in culture

Kothlow, Sonja; Morgenroth, Iris; Tregaskes, Clive A.; Kaspers, Bernd; Young, J. R.

doi:10.1016/j.dci.2008.01.012

Cited by 34 publications

(34 citation statements)

References 43 publications

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“…Chicken primary B cells can be cultured in the presence of chicken CD40L Consistent with previous reports [11,19], we found that when chicken primary B cells were cultured in the presence of soluble chCD40L, the number and viability of the cells was significantly increased compared to when cells were cultured in the absence of chCD40L (Fig. 1).…”

Section: Resultssupporting

confidence: 92%

“…Once digested, the tissue was passed through a 100 µM Falcon cell strainer (Thermo Fisher Scientific) into cell medium containing Hanks balanced salt solution (Gibco) supplemented with 7.5 % sodium carbonate and 500 mM EDTA (Sigma-Aldrich), before pelleting. Cells were resuspended in Iscove's modified Dulbecco's medium (IMDM) supplemented with FBS, chicken serum (SigmaAldrich), insulin transferrin selenium (Gibco), beta-mercaptoethanol (Gibco) and penicillin/streptomycin and nystatin (B-cell media; as described by [19]), before undergoing centrifugation at 2000 r.p.m. for 20 min at 4 C over Histopaque 1083 (Sigma-Aldrich).…”

Section: Chicken Primary B Cell Culturementioning

confidence: 99%

“…The gene encoding chicken CD40 ligand (chCD40L), a molecule responsible for B-cell proliferation in vivo, was identified [18] and a soluble fusion protein containing its extracellular domain was subsequently shown to support the proliferation of chicken B cells in culture for up to three weeks [19]. In 2015, Schermuly et al showed that chCD40L-treated B cells could be infected with Marek's disease virus [11], demonstrating that the cells have the potential for use in the study of the consequences of lymphotropic virus infection.…”

Section: Introductionmentioning

confidence: 99%

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Differential gene expression in chicken primary B cells infected ex vivo with attenuated and very virulent strains of infectious bursal disease virus (IBDV)

Dulwich

Giotis

Gray

et al. 2017

Journal of General Virology

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Infectious bursal disease virus (IBDV) belongs to the family Birnaviridae and is economically important to the poultry industry worldwide. IBDV infects B cells in the bursa of Fabricius (BF), causing immunosuppression and morbidity in young chickens. In addition to strains that cause classical Gumboro disease, the so-called 'very virulent' (vv) strain, also in circulation, causes more severe disease and increased mortality. IBDV has traditionally been controlled through the use of live attenuated vaccines, with attenuation resulting from serial passage in non-lymphoid cells. However, the factors that contribute to the vv or attenuated phenotypes are poorly understood. In order to address this, we aimed to investigate host cell-IBDV interactions using a recently described chicken primary B-cell model, where chicken B cells are harvested from the BF and cultured ex vivo in the presence of chicken CD40L. We demonstrated that these cells could support the replication of IBDV when infected ex vivo in the laboratory. Furthermore, we evaluated the gene expression profiles of B cells infected with an attenuated strain (D78) and a very virulent strain (UK661) by microarray. We found that key genes involved in B-cell activation and signalling (TNFSF13B, CD72 and GRAP) were down-regulated following infection relative to mock, which we speculate could contribute to IBDV-mediated immunosuppression. Moreover, cells responded to infection by expressing antiviral type I IFNs and IFNstimulated genes, but the induction was far less pronounced upon infection with UK661, which we speculate could contribute to its virulence.

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Section: Resultssupporting

confidence: 92%

Section: Chicken Primary B Cell Culturementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Differential gene expression in chicken primary B cells infected ex vivo with attenuated and very virulent strains of infectious bursal disease virus (IBDV)

Dulwich

Giotis

Gray

et al. 2017

Journal of General Virology

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“…However, using a combination of CD40 stimulation in the presence of interleukin (IL)-2, IL-4 and IL-10 appears to be a good stimulus for expansion of human and other animal B lymphocytes in vitro [7][8][9][10], and also plays a significant role in the normal development and function of B lymphocytes [11][12][13][14] as well as in the propagation of malignant B lymphocytes [15]. The exact signalling pathways responsible for the induction and maintenance of B lymphocytes proliferation are unknown, but previous studies have implicated a role for the c-Src protein tyrosine kinase [9].…”

Section: Introductionmentioning

confidence: 99%

c-Src tyrosine kinase co-associates with and phosphorylates signal transducer and activator of transcription 5b which mediates the proliferation of normal human B lymphocytes

Cayer

Proulx

et al. 2009

Clinical and Experimental Immunology

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“…It has been well established that the CD40L of T cells is a key molecule for B cell proliferation and Ig production, especially Ig class switching (18). In order to exclude the possibility that B cell proliferation induced by fNE-treated DCs is due to CD40-CD40L interaction from residual T cells in B cell preparation, saturated anti-CD40L monoclonal antibody was used to neutralize CD40L in a DC/B cell co-culture system.…”

Section: B Cell Proliferation Stimulated By Fne-treated Dcs Is Cd40l-mentioning

confidence: 99%

Fermented Noni Exudate-treated dendritic cells directly stimulate B lymphocyte proliferation and differentiation

Zhang

Li²,

Wong³

et al. 2009

Oncol Rep

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Abstract. Noni juice as a folk medicine has been used for over two thousand years. Recently, some active ingredients of Noni juice have been successfully isolated and intensively studied. Because dendritic cells (DCs) are central regulators both in priming innate and adaptive immune responses and in maintaining self tolerance, in the current study we treated DCs with fermented Noni Exudate (fNE) in order to explore their function in regulating other immune cells. It was shown that fNE-treated DCs stimulate proliferation of splenocytes, among which, B cells are the major responsive cell group. The proliferative response of B cells to fNE-treated DCs is cell contact-dependent, CD40L-independent; and the adhesion feature of DCs was enhanced to form large DC-B conjugation cluster. Moreover, it was demonstrated that fNE-treated DCs promote B cell differentiation and Ig class switching. These results lay a foundation for the further exploration of fNE as a biological response modifier in the immune system.

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CD40 ligand supports the long-term maintenance and differentiation of chicken B cells in culture

Cited by 34 publications

References 43 publications

Differential gene expression in chicken primary B cells infected ex vivo with attenuated and very virulent strains of infectious bursal disease virus (IBDV)

Differential gene expression in chicken primary B cells infected ex vivo with attenuated and very virulent strains of infectious bursal disease virus (IBDV)

c-Src tyrosine kinase co-associates with and phosphorylates signal transducer and activator of transcription 5b which mediates the proliferation of normal human B lymphocytes

Fermented Noni Exudate-treated dendritic cells directly stimulate B lymphocyte proliferation and differentiation

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