CD4⁺ and CD8⁺ T‐Cell‐Specific DNA Cytosine Methylation Differences Associated With Obesity

Abstract

The methylomes of various leukocytes respond differently to obesity and levels of visceral adipose tissue. Highly significant differentially methylated sites in CD4 and CD8 cells in women with obesity that have apparent biological relevance to obesity were identified.

The Association Between DNA Methylation of CASZ1 Gene and Overweight/obesity in the Chinese Han Population

“…Recent investigation indicated that changes in DNA methylation were consequences of adiposity at the majority of the identi ed CpG sites [27] The mechanism by which obesity may be associated with methylation of CASZ1 was unclear. The methylation at a speci c CpG (cg17177074) of CASZ1 gene was identi ed to be associated with visceral adipose tissue in CD4 + T cells [20]. CASZ1 gene was highly expressed in CD4 + T cells , and can promote the expression of T-helper type 17 (Th17) genes by in uencing chromatin modi cations in Th17 lineage genes [28].…”

“…A critical region at the CASZ1 N terminus (AAs 23-40) was identi ed to suppress NB tumorigenesis by mediating the interaction between CASZ1b nuclear localization and NuRD, [36]. A case had ROHHAD syndrome showed that obesity may be associated with neuroblastoma, but the mechanism has still not been clari ed [20]. To con rm the mechanism between CASZ1 methylation and obesity, further research remains to be conducted.…”

“…A whole-genome linkage scan showed that 1p36 was associated with BMI [19]. According to the latest study, the CASZ1 methylation in CD4 + T cells was strongly associated with the amount of visceral adipose tissue [20]. In this context, CASZ1 gene methylation may play a role in obesity.…”

Birthweight DNA methylation signatures in infant saliva

“…In the EWAS Atlas, DNA methylation in 29 out of these 87 CpGs was associated Tables S1 and S2). DNA methylation variation at eleven of them has been previously associated with insulin resistance [53] (cg03045325), colorectal cancer [54] (cg02727104, cg26332310, cg12322146), obesity [55] (cg03066788), bariatric surgery [56] (cg20515787, cg02547025), mortality [57] (cg06234201), gestational diabetes mellitus [58] (cg00383136), amount of visceral adipose tissue [59] (cg20388707), and gestational age [34] (cg00701706). Some of the birthweight-and SGA-associated CpGs in our study map in genes which variants were associated with the following traits: birthweight (USH2A), BMI (CENPO, E2F3, RPTOR, SNTB2, PNOC, LGR4), body fat distribution (CENPO), waist-hip ratio (SYTL2, ZNF423, FOXA3, LMNB2, COL5A1,LGR4), BMI-adjusted waist hip ratio (ZNF423, AFF3), cardiovascular disease (SIPA1L2, FOXA3, RAB37, INPP5A), subcutaneous or visceral adipose tissue measurement (FOXA3, RPTOR), gestational age (CFAP46, INPP5A), lipoprotein cholesterol measurement (DMTN, SNTB2), total cholesterol measurement (E2F3, DMTN), type I diabetes nephropathy (AFF3), type II diabetes mellitus (RAMP1, SYCE1L, ZNF710).…”

Birthweight DNA Methylation Signatures in Infant Saliva 

“…In the EWAS Atlas, 29 out of 87 birthweight-and SGA-associated CpGs in our study were associated with different traits (Additional le 1 -S2 and S3 Tables). DNA methylation variation at eleven of them has been previously associated with insulin resistance [51] (cg03045325), colorectal cancer [52] (cg02727104, cg26332310, cg12322146), obesity [53](cg03066788), bariatric surgery [54](cg20515787, cg02547025), mortality [55] (cg06234201), gestational diabetes mellitus [56] (cg00383136), amount of visceral adipose tissue [57] (cg20388707), and gestational age [32] (cg00701706).…”