2024
DOI: 10.1101/2024.05.01.592003
|View full text |Cite
Preprint
|
Sign up to set email alerts
|

CD4 downregulation precedes Env expression and protects HIV-1-infected cells from ADCC mediated by non-neutralizing antibodies

Jonathan Richard,
Gérémy Sannier,
Li Zhu
et al.

Abstract: HIV-1 envelope glycoprotein (Env) conformation substantially impacts antibody-dependent cellular cytotoxicity (ADCC). Envs from primary HIV-1 isolates adopt a prefusion closed conformation, which is targeted by broadly-neutralizing antibodies (bnAbs). CD4 binding drives Env into more open conformations, which are recognized by non-neutralizing Abs (nnAbs). To better understand Env-Ab and Env-CD4 interaction in CD4+ T cells infected with HIV-1, we simultaneously measured antibody binding and HIV-1 mRNA expressi… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
1
1

Citation Types

1
1
0

Year Published

2024
2024
2024
2024

Publication Types

Select...
1

Relationship

1
0

Authors

Journals

citations
Cited by 1 publication
(2 citation statements)
references
References 107 publications
(221 reference statements)
1
1
0
Order By: Relevance
“…However, upon addition of the indoline CD4mc CJF-III-288, recognition by all plasma was significantly enhanced. This enhanced recognition translated into a marked increase in ADCC activity (Figure 1B), as measured with a previously described FACS-based ADCC assay (11,13,27). As expected, recognition of infected cells by plasma samples was significantly associated with their ADCC activity (Figure 1C).…”
Section: Cjf-iii-288 Enhances the Capacity Of Plasma From Plwh To Rec...supporting
confidence: 79%
See 1 more Smart Citation
“…However, upon addition of the indoline CD4mc CJF-III-288, recognition by all plasma was significantly enhanced. This enhanced recognition translated into a marked increase in ADCC activity (Figure 1B), as measured with a previously described FACS-based ADCC assay (11,13,27). As expected, recognition of infected cells by plasma samples was significantly associated with their ADCC activity (Figure 1C).…”
Section: Cjf-iii-288 Enhances the Capacity Of Plasma From Plwh To Rec...supporting
confidence: 79%
“…Anti-human IgG Fc secondary antibodies (Thermo Fisher Scientific) were used when cell surface binding was performed in presence of Fab fragment blockade. The PE-conjugated anti-HIV p24 Ab (Clone KC57-RD1, Beckman Coulter) and the FITC anti-human CD4 OKT4 Antibody (Biolegend) were used to detect productively HIV-1 infected cells in flow cytometry cell-surface staining and ADCC assays (27).…”
Section: Antibodiesmentioning
confidence: 99%