CD4 down-modulation by ganglioside and phorbol ester inhibits human herpesvirus 7 infection

Yasukawa, Masaki; Inoue, Yasuhiro; Sada, Eiji; Yakushijin, Yoshihiro; Furukawa, Maho; Fujita, Shigeru

doi:10.1099/0022-1317-76-9-2381

Cited by 15 publications

(10 citation statements)

References 16 publications

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“…CD8 ϩ T cell cross-reactivity in humans has been recently documented between nonhomologous viruses, including influenza A virus with hepatitis C virus (33). While no study documents T cell cross-reactivity between human ␣-herpesviruses (VZV and HSV), human CD4 ϩ T cell cross-reactivity has been detected between human ␤-herpesviruses, namely CMV, HHV-6, and HHV-7, although this is rare (34). In at least one IS subject (patient 1), T cell cross-reactivity seems unlikely, since four CD4 ϩ T cell clones isolated from this subject recognized four different epitopes from four different HSV-2 proteins.…”

Section: Discussionmentioning

confidence: 99%

T Cell Immunity to Herpes Simplex Viruses in Seronegative Subjects: Silent Infection or Acquired Immunity?

Posavad

Wald

Hosken³

et al. 2003

The Journal of Immunology

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During the course of investigating T cell responses to HSV among volunteers entering trials of investigational genital herpes vaccines, 6 of the 24 immunocompetent subjects with no prior history of oral/labial or genital herpes possessed HSV-specific T cell immunity but, by multiple determinants of even the most sensitive serological assays, remained seronegative to HSV-1 and -2. Of these six immune seronegative (IS; HSV-seronegative with HSV-specific T cell responses) subjects, two had transient HSV-specific T cell responses, while four had CD4+ and CD8+ T cell responses directed at HSV that persisted for up to 4 years. CD4+ T cell clones were isolated that recognized and had high binding affinities to epitopes in HSV-2 tegument proteins. All six IS subjects had potential sexual exposure to an HSV-2-infected sexual partner. Oral and genital mucosal secretions were sampled and tested for the presence of infectious HSV and HSV DNA. No evidence of HSV was detected in >1500 samples obtained from these IS subjects. The identification of persistent T cell responses to HSV in seronegative subjects is a novel finding in the herpesvirus field and suggests either undetected infection or acquired immunity in the absence of infection. Understanding the basis of these acquired immune responses may be critical in developing effective vaccines for genital herpes.

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Section: Discussionmentioning

confidence: 99%

T Cell Immunity to Herpes Simplex Viruses in Seronegative Subjects: Silent Infection or Acquired Immunity?

Posavad

Wald

Hosken³

et al. 2003

The Journal of Immunology

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“…Therefore, HHV-7 infection is possible in the presence of antibody to HHV-6. It is, however, not clear whether there is some modification of clinical symptoms due to the prior infection, because the cross-reactivity of cellular immunity has also been reported (Yasukawa et al, 1993).…”

Section: Discussionmentioning

confidence: 95%

Antigenic analysis of human herpesvirus 7 (HHV-7) and HHV-6 using immune sera and monoclonal antibodies against HHV-7.

Nakagawa

Mukai²,

Sakamoto³

et al. 1997

Journal of General Virology

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Using polyclonal and monoclonal (MAbs) antibodies to human herpesvirus 7 (HHV-7), we have studied HHV-7-specific polypeptides. Human sera were obtained during the convalescent phase from patients with exanthem subitum due to HHV-7, and at least 16 HHV-7-specific polypeptides with apparent molecular masses of 26-210 kDa were immunoprecipitated. Sera prepared in mice also precipitated at least 17 HHV-7-specific polypeptides with molecular masses of 26-210 kDa. Among them, the most commonly observed antigenic protein had an apparent molecular mass of 52 kDa. Forty-two clones secreting MAbs against HHV-7-specific proteins, as determined by immunofluorescence assays, were established from BALB/c mice immunized

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“…Differences in suppressive effects on colony formation between HHV-6 and HHV-7 might partly be attributed to the difference in the tropism between these viruses [Lusso et al,1991]. Specifically, HHV-7 uses the CD4 molecule as a receptor for the entry to the host cells [Lusso et al, 1994;Yasukawa et al, 1995]. It is likely that the lack of effect of this virus on hematopoietic colony formation is due to the inability of the virus to infect hematopoietic progenitor cells.…”

Section: Discussionmentioning

confidence: 97%

Suppressive effects of human herpesvirus 6 on in vitro colony formation of hematopoietic progenitor cells

et al. 1997

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Human herpesvirus 6 (HHV-6) has been reported to be involved in bone marrow failure after bone marrow transplantation (BMT). To elucidate the role of HHV-6 in the marrow failure, we examined the comparative effect of two variants of HHV-6 (HHV-6A and HHV-6B) and human herpesvirus 7 (HHV-7) on in vitro colony formation of hematopoietic progenitor cells in methylcellulose semi-solid media. Progenitor cells prepared from cord blood mononuclear cells (CBMNCs) were infected with one of these viruses at various multiplicity of infection (MOI), and were subjected to methylcellulose colony assay. Formation of both granulocyte/macrophage (CFU-GM) and erythroid (BFU-E) colonies was MOI-dependently suppressed after infection with the Z29 strain of HHV-6B. Although HHV-6A suppressed the formation of BFU-E colonies as efficiently as HHV-6B, the former did not exhibit significant suppressive effect on the formation of CFU-GM colonies at an MOI 1. HHV-7 had no effect on hematopoietic colony formation at all. Based on frequent positivity of viral DNA in single colonies obtained from HHV-6-infected progenitor cells by polymerase chain reaction and in situ hybridization, direct effects of HHV-6 on the hematopoietic progenitor cells are suggested as the cause of the suppression rather than indirect effects via accessory cells of the bone marrow.

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CD4 down-modulation by ganglioside and phorbol ester inhibits human herpesvirus 7 infection

Cited by 15 publications

References 16 publications

T Cell Immunity to Herpes Simplex Viruses in Seronegative Subjects: Silent Infection or Acquired Immunity?

T Cell Immunity to Herpes Simplex Viruses in Seronegative Subjects: Silent Infection or Acquired Immunity?

Antigenic analysis of human herpesvirus 7 (HHV-7) and HHV-6 using immune sera and monoclonal antibodies against HHV-7.

Suppressive effects of human herpesvirus 6 on in vitro colony formation of hematopoietic progenitor cells

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