CD4−8− T-cells increase in MRL/lpr mice treated with thymic factors

Mattei, Maurizio; Bach, Simona; Cesare, Silvia Di; Fraziano, Maurizio; Placido, Roberta; Poccia, Fabrizio; Sammarco, Innocenzo; Morás, Ana Moira; Bardone, M.R.; Colizzi, Vittorio

doi:10.1016/0192-0561(94)90138-4

Cited by 6 publications

(3 citation statements)

References 18 publications

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“…The origin of these cells is not clearly de®ned. Early studies propose that these abnormal T cells originate from thymic precursors (41,42). More recent work suggests that these cells are post-activated T cells surviving due to lack of functional Fas signaling (43±45), as T cells undergoing superantigen-induced apoptosis in vivo express B220 and Fas (46), and Tg expression of Fas in T cells blocks lymphoproliferation in MRL/lpr mice (47).…”

Section: Discussionmentioning

confidence: 99%

Increased plasma cell frequency and accumulation of abnormal syndecan-1plus T-cells in Ig -deficient/lpr mice

Seagal¹

2003

International Immunology

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The expression of muH chain is an important checkpoint in B cell development. In mice deficient for IgM transmembrane tail exons (muMT mice) B cell development is blocked at the pro-B stage. However, we showed that Fas-deficient muMT mice (muMT/lpr) develop a very small population of isotype-switched B cells and produce high titers of self-reactive serum antibodies. In addition, muMT/lpr mice develop severe lymphoproliferation and both pathologic processes occur at young ages. This may suggest that lack of Fas-Fas ligand signaling exacerbates murine lupus in B cell lymphopenic mice. To test this we analyzed antibody and plasma cell formation, and accumulation of abnormal T cells in muMT/lpr mice. Our results show that the muMT/lpr mouse is particularly permissive for the development and accumulation of antibody-producing cells, thereby explaining the high titers of serum antibodies in these mice. In addition, we found that accumulating cells in spleen and lymph nodes of muMT/lpr mice are alphabeta T cells expressing the abnormal B220+/CD3+ surface markers, a phenotype also described for other Fas-deficient mouse models. Strikingly, we found that accumulating cells in muMT/lpr mice express the membrane proteoglycan syndecan-1, a known plasma cell marker. Development of these cells is blocked in mice deficient for TCRbeta and TCRdelta. We also found that both antibody production and lymphoproliferation in muMT/lpr mice are Th1 regulated. Our results, therefore, suggest that in the muMT/lpr mouse model a small population of isotype-switched B cells is sufficient for the initiation and propagation of Th1-regulated murine lupus.

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Section: Discussionmentioning

confidence: 99%

Increased plasma cell frequency and accumulation of abnormal syndecan-1plus T-cells in Ig -deficient/lpr mice

Seagal¹

2003

International Immunology

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“…This peptide is used for the treatment of autoimmune diseases such as chronic lymphatic leukemia, rheumatoid arthritis, and atopic dermatitis. Moreover the peptide causes the induction of T cell differentiation. − Potential therapeutic effects of TP5 as well as its sequence that contains a variety of functional groups that are present in other therapeutic peptides and proteins brought us to use it as a model for preparing peptide imprinted polymeric networks. In the present work, the effect of the initiator/catalyst (APS)/TEMED on the integrity of thymopentin under conditions used for preparation of imprinted hydrogels was investigated …”

Section: Introductionmentioning

confidence: 99%

Methyleneation of Peptides by N,N,N,N-Tetramethylethylenediamine (TEMED) under Conditions Used for Free Radical Polymerization: A Mechanistic Study

et al. 2014

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Free radical polymerization is often used to prepare protein and peptide-loaded hydrogels for the design of controlled release systems and molecular imprinting materials. Peroxodisulfates (ammonium peroxodisulfates (APS) or potassium peroxodisulfates (KPS)) with N,N,N,N-tetramethylethylenediamine (TEMED) are frequently used as initiator and catalyst. However, exposure to these free radical polymerization reagents may lead to modification of the protein and peptide. In this work, we show the modification of lysine residues by ammonium peroxodisulfate (APS)/TEMED of the immunostimulant thymopentin (TP5). Parallel studies on a decapeptide and a library of 15 dipeptides were performed to reveal the mechanism of modification. LC-MS of APS/TEMED-exposed TP5 revealed a major reaction product with an increased mass (+12 Da) with respect to TP5. LC-MS(2) and LC-MS(3) were performed to obtain structural information on the modified peptide and localize the actual modification site. Interpretation of the obtained data demonstrates the formation of a methylene bridge between the lysine and arginine residue in the presence of TEMED, while replacing TEMED with a sodium bisulfite catalyst did not show this modification. Studies with the other peptides showed that the TEMED radical can induce methyleneation on peptides when lysine is next to arginine, proline, cysteine, aspargine, glutamine, histidine, tyrosine, tryptophan, and aspartic acid residues. Stability of peptides and protein needs to be considered when using APS/TEMED in in situ polymerization systems. The use of an alternative catalyst such as sodium bisulfite may preserve the chemical integrity of peptides during in situ polymerization.

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“…A fascinating but still controversial recent observation of IL‐17‐containing CD4 – 8 – T cells in kidney tissues of SLE patients with nephritis is complicated by the lack of differences in IL‐17 positivity of CD4 – 8 – T cells in blood from SLE patients compared to normal control subjects, whether T cells were freshly isolated, cultured, or stimulated in culture (12). The greatest increases in blood and tissue levels of TCRαβ CD4 – 8 – T cells are found in patients with the autoimmune lymphoproliferative syndrome (ALPS) and MRL/lpr mice with a similar syndrome (13–15). ALPS is attributable to diverse defects in lymphocyte Fas‐mediated apoptosis leading to lymphadenopathy, splenomegaly, B lymphocytosis, hypergamma globulinemia, and numerous immune cytopenias, in association with very high circulating levels of TCRαβ CD4 – 8 – T cells that may rise to 1000/µl and thus represent 25–30% of total T cells (14).…”

mentioning

confidence: 99%

Human CD4 8 T cells are a distinctive immunoregulatory subset

et al. 2010

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Human CD4(-)8(-) T cells are a minor subset quantitatively but potentially important in immunity because they are predominantly distributed at body surfaces, and their number and activities increase in autoimmune diseases and decrease with aging. Distinguishing characteristics of CD4(-)8(-) T cells are found to include a unique profile of cytokines, including Serpin E1, which is not generated by other T cells, MIF, and TGF-beta. At 2-5% of the total in mixtures with CD4 + CD8 T cells, CD4(-)8(-) T cells enhance the generation of IFN-gamma and IL-17 by up to 12- and 5-fold, respectively, without contributing either cytokine or affecting cytokine production by NK/NKT cells. CD4(-)8(-) T cell-derived MIF is their major enhancer and TGFbeta their principal inhibitor of CD4 and CD8 T cell cytokine production. Decreases in CD4(-)8(-) T cell effects may diminish protective immunity in aging, whereas increases may augment the severity of autoimmune diseases.

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CD4−8− T-cells increase in MRL/lpr mice treated with thymic factors

Cited by 6 publications

References 18 publications

Increased plasma cell frequency and accumulation of abnormal syndecan-1plus T-cells in Ig -deficient/lpr mice

Increased plasma cell frequency and accumulation of abnormal syndecan-1plus T-cells in Ig -deficient/lpr mice

Methyleneation of Peptides by N,N,N,N-Tetramethylethylenediamine (TEMED) under Conditions Used for Free Radical Polymerization: A Mechanistic Study

Human CD4 8 T cells are a distinctive immunoregulatory subset

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