CD36‐mediated endocytic uptake of advanced glycation end products (AGE) in mouse 3T3‐L1 and human subcutaneous adipocytes

Kuniyasu, Akihiko; Ohgami, Nobutaka; Hayashi, Shigeki; Miyazaki, Akira; Horiuchi, Seikoh; Nakayama, Hitoshi

doi:10.1016/s0014-5793(03)00096-6

Cited by 84 publications

(59 citation statements)

References 36 publications

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“…Adipocytes express CD36, a scavenger receptor that binds AGEs (incl. CML) and facilitates endocytosis and degradation 21,22 . Thus, fat mass could influence circulating AGE concentrations through a role in the catabolism and breakdown of AGE.…”

Section: Discussionmentioning

confidence: 99%

Serum carboxymethyl-lysine, a dominant advanced glycation end product, is increased in women with gestational diabetes mellitus

Bartáková¹,

Kollárová²,

Kuricová³

et al. 2016

BIOMED PAP

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Aims. The objective of the study was to measure one of the circulating Advanced Glycation End Products (AGEs) -Nε-(carboxymethyl)lysine (CML) -in a case-control study (n = 307) of pregnant women with gestational diabetes mellitus (GDM) and physiological pregnancies and to ascertain the factors contributing to CML levels and the potential relevance of CML for selected perinatal and postpartum outcomes. Methods. All subjects underwent oGTT between 24 th and 30 th week of gestation and GDM was diagnosed according to WHO criteria. CML was determined by ELISA using commercial kit. Results. Unadjusted and plasma protein adjusted CML levels were significantly higher in women with GDM compared to healthy controls (P = 0.00043 and P = 1x10 -5 , respectively, Mann-Whitney). CML was significantly inversely correlated with both pre-and mid-gestational BMI, however, differences between GDM and control group remained significant even after adjustment for BMI. CML levels correlated with 1-h and 2-h post-load glycaemia during oGTT. Conclusion. In conclusion, we found statistically significantly higher protein-and BMI-normalised CML levels measured during 24-30 th week of gestation in women with GDM compared to healthy pregnant controls. Further studies are warranted to comprehensively asses the spectrum of AGEs in GDM and their relevance to future metabolic health of mother and offspring.

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Section: Discussionmentioning

confidence: 99%

Serum carboxymethyl-lysine, a dominant advanced glycation end product, is increased in women with gestational diabetes mellitus

Bartáková¹,

Kollárová²,

Kuricová³

et al. 2016

BIOMED PAP

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“…125 I-rHuTWEAK was prepared by using an Iodogen coating tube (Pierce) as described (35). Briefly, 200 g of rHuTWEAK was incubated with Na 125 I (0.925 MBq) in 200 l of 0.5 M sodium phosphate buffer (pH 7.4) for 20 min at 37°C, and then dialyzed against PBS.…”

Section: Preparation Of 125 I-rhutweak and Hp-hb Complexesmentioning

confidence: 99%

“…Cellular binding of 125 I-rHuTWEAK was performed in accordance to that of advanced glycated end product-modified BSA by CD36 as described previously (35). For cell binding assay, cells were incubated with 100 ng/ml 125 I-rHuTWEAK at 4°C in RPMI 1640 containing 3% BSA for 3 h. After incubation, cells were washed three times with ice-cold PBS and lysed with 0.1 M NaOH.…”

Section: Cellular Binding Of 125 I-rhutweak By Cd163-cho Cellsmentioning

confidence: 99%

A Previously Unrecognized Protein-Protein Interaction between TWEAK and CD163: Potential Biological Implications

Bover

Cardó-Vila

Kuniyasu³

et al. 2007

The Journal of Immunology

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190

183

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TWEAK (TNF-like weak inducer of apoptosis) is a TNF superfamily member implicated in several mechanisms. Although fibroblast growth factor inducible 14 (Fn14)/TweakR has been reported as its receptor, an as yet unrecognized surface molecule(s) might modulate TWEAK function(s). Thus, we set out to identify TWEAK-binding proteins by screening a combinatorial peptide library. Cyclic peptides containing a consensus motif (WXDDG) bound to TWEAK specifically. These peptides were similar to CD163, a scavenger receptor cysteine-rich domain family member, restricted to the monocyte/macrophage lineage and responsible for the uptake of circulating haptoglobin-hemoglobin (Hp-Hb) complexes. Sequence profile analysis suggested that TWEAK mimicked the CD163 natural ligand (Hp-Hb). Consistently, we show dose-dependent TWEAK binding to CD163 and blockade by an anti-CD163 Ab. In a competition assay, both soluble CD163 and Fn14/TweakR were able to compete off TWEAK binding to coated Fn14/TweakR or CD163, respectively. Flow-cytometry and immunofluorescence assays showed that human monocytes (Fn14/TweakR negative and CD163 positive) bind TWEAK, thus blocking the recognition of CD163 and reducing the activation mediated by a specific mAb in these cells. We demonstrate that monocytes can sequester TWEAK from supernatants, thus preventing tumor cell apoptosis; this effect was reverted by preincubation with the peptide mimicking CD163 or with a mAb anti-CD163, indicating specificity. Finally, we show that recombinant human TWEAK binding to CD163-transfected Chinese hamster ovary cells is inhibited by the presence of either unlabeled TWEAK or the Hp-Hb complex. Together, these data are consistent with the hypothesis that CD163 either acts as a TWEAK scavenger in pathological conditions or serves as an alternate receptor for TWEAK in cells lacking Fn14/TweakR.

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“…We have shown that dietary AGEs acutely impair endothelial function (18,19), an effect counteracted by benfotiamine (20), a transketolase activator that blocks several hyperglycemiainduced pathways, including the formation of AGEs (21). AGEs might interact with adipocytes through AGE receptors (22) and induce cellular dysfunction via generation of reactive oxygen species (23), a pathway probably responsible for the AGE-induced downregulation of leptin secretion in vitro (24).…”

Section: Diethelm Tschoepe MDmentioning

confidence: 99%

Adiponectin Decreases Postprandially Following a Heat-Processed Meal in Individuals With Type 2 Diabetes

et al. 2007

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CD36‐mediated endocytic uptake of advanced glycation end products (AGE) in mouse 3T3‐L1 and human subcutaneous adipocytes

Cited by 84 publications

References 36 publications

Serum carboxymethyl-lysine, a dominant advanced glycation end product, is increased in women with gestational diabetes mellitus

Serum carboxymethyl-lysine, a dominant advanced glycation end product, is increased in women with gestational diabetes mellitus

A Previously Unrecognized Protein-Protein Interaction between TWEAK and CD163: Potential Biological Implications

Adiponectin Decreases Postprandially Following a Heat-Processed Meal in Individuals With Type 2 Diabetes

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