CD36 and SR-BI Are Involved in Cellular Uptake of Provitamin A Carotenoids by Caco-2 and HEK Cells, and Some of Their Genetic Variants Are Associated with Plasma Concentrations of These Micronutrients in Humans

Borel, Patrick; Lietz, Georg; Goncalves, Aurélie; Edelenyi, Fabien Szabo de; Lecompte, Sophie; Curtis, Peter J.; Goumidi, Louisa; Caslake, Muriel; Miles, Elizabeth A.; Packard, Christopher J.; Calder, Philip C.; Mathers, John C.; Minihane, Anne‐Marie; Tourniaire, Franck; Kesse‐Guyot, Emmanuelle; Galán, Pilar; Herçberg, Serge; Breidenassel, Christina; Gross, Marcela González; Moussa, Myriam; Meirhaeghe, Aline; Reboul, Emmanuelle

doi:10.3945/jn.112.172734

Cited by 121 publications

(132 citation statements)

References 43 publications

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“…On the other hand, CD36 contributes to cellular uptake of b-carotene (35), and the mRNA levels of CD36 are higher in soleus muscle than in EDL muscle in rats (36). Furthermore, dietary b-carotene decreases the levels of thiobarbituric acid-reactive substances, which are an indicator of lipid peroxidation, in soleus muscle, but not in gastrocnemius muscle (15), suggesting that b-carotene acts on soleus muscle as an antioxidant.…”

Section: Discussionmentioning

confidence: 92%

β-Carotene Increases Muscle Mass and Hypertrophy in the Soleus Muscle in Mice

Kitakaze

Harada

Imagita

et al. 2015

Journal of Nutritional Science and Vitaminology, J Nutr Sci Vit

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Summary Supplements and naturally occurring nutraceuticals effective for maintenance or enhancement of skeletal muscle mass are expected to contribute to prevention of decreased mobility and increased risk of developing metabolic diseases. However, information about available food components remains widely unavailable. In the present study, we investigated the effects of dietary b-carotene on the quantity and quality of skeletal muscle under physiological conditions. Male ddY mice (8 wk old) were orally administered b-carotene (0.5 mg once daily) for 14 d. Dietary b-carotene had no influence on body weight, but increased the soleus muscle/body weight ratio. The cross-sectional area (CSA) in muscle fibers of the soleus muscle was increased, indicating that administration of b-carotene induces muscle hypertrophy. In the soleus muscle of the b-carotene-administered mice, twitch force tended to be increased (p50.06) and tetanic force was significantly increased, whereas specific force (force per CSA) remained unchanged. Dietary b-carotene increased the mRNA level of insulin-like growth factor 1 (Igf-1) as its splicing variant Igf-1ea, but had no influence on the liver Igf-1 mRNA level or serum IGF-1 level. b-Carotene promoted protein synthesis in the soleus muscle and reduced levels of ubiquitin conjugates, but had no influence on the mRNA levels of two atrogenes, Atrogin-1 and Murf1. On the other hand, b-carotene had no influence on the processing of the autophagy marker protein light chain 3. These results indicate that in mice, administration of b-carotene increases mass and induces functional hypertrophy in the soleus muscle, perhaps by promoting IGF-1-mediated protein synthesis and by reducing ubiquitin-mediated protein degradation.

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Section: Discussionmentioning

confidence: 92%

β-Carotene Increases Muscle Mass and Hypertrophy in the Soleus Muscle in Mice

Kitakaze

Harada

Imagita

et al. 2015

Journal of Nutritional Science and Vitaminology, J Nutr Sci Vit

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“…SCARB1 was first characterized in humans as a high-density lipoprotein receptor that mediates the selective uptake of cholesterol, and was subsequently demonstrated to have a role in the cellular uptake of a variety of lipids, including phospholipids, products of triglycerol hydrolysis, and the lipophilic vitamins A and D (28,35,36). SCARB1 homologs have now been implicated as mediators of carotenoid uptake in fruit flies (Drosophila melanogaster), silkworms (Bombyx mori), salmon (Salmo salar), mice, and humans (24,26,(37)(38)(39)(40)(41). These findings suggest that SCARB1 is an ancient and conserved mechanism of carotenoid uptake in animals.…”

Section: Discussionmentioning

confidence: 99%

High-density lipoprotein receptor SCARB1 is required for carotenoid coloration in birds

Toomey

Lopes

Araújo

et al. 2017

Proc. Natl. Acad. Sci. U.S.A.

115

104

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Yellow, orange, and red coloration is a fundamental aspect of avian diversity and serves as an important signal in mate choice and aggressive interactions. This coloration is often produced through the deposition of diet-derived carotenoid pigments, yet the mechanisms of carotenoid uptake and transport are not wellunderstood. The white recessive breed of the common canary (Serinus canaria), which carries an autosomal recessive mutation that renders its plumage pure white, provides a unique opportunity to investigate mechanisms of carotenoid coloration. We carried out detailed genomic and biochemical analyses comparing the white recessive with yellow and red breeds of canaries. Biochemical analysis revealed that carotenoids are absent or at very low concentrations in feathers and several tissues of white recessive canaries, consistent with a genetic defect in carotenoid uptake. Using a combination of genetic mapping approaches, we show that the white recessive allele is due to a splice donor site mutation in the scavenger receptor B1 (SCARB1; also known as SR-B1) gene. This mutation results in abnormal splicing, with the most abundant transcript lacking exon 4. Through functional assays, we further demonstrate that wild-type SCARB1 promotes cellular uptake of carotenoids but that this function is lost in the predominant mutant isoform in white recessive canaries. Our results indicate that SCARB1 is an essential mediator of the expression of carotenoid-based coloration in birds, and suggest a potential link between visual displays and lipid metabolism.coloration | carotenoids | lipid metabolism | Serinus canaria T he yellow, orange, and red coloration of the feathers, skin, and beaks of birds is most commonly produced through the deposition of carotenoid pigments (1). Carotenoid coloration of birds has been a focus of study in the fields of behavior, evolution, and physiology because it plays a key role in mate assessment in many species. In addition, it is frequently an indicator of individual quality, and can signal species identity (2-4). Birds cannot synthesize carotenoids de novo and must acquire them through their diet (1), potentially linking coloration to the acquisition of pigments from the environment (3). Thus, key hypotheses related to honest signaling and sexual selection have been shaped by and are currently being tested in carotenoidornament systems (5, 6). Ultimately, the information content and evolutionary trajectories of carotenoid ornaments are a function of the physiological mechanisms underlying color expression, yet our understanding of these mechanisms is limited.The expression of carotenoid coloration in birds involves four distinct physiological steps: uptake in the gut, transport in circulatory and lymphatic systems, metabolism either at the site of deposition or in the liver, and deposition in the integument (7). Recent progress has been made in understanding how carotenoids are metabolized to novel forms. In 2016, two studies independently identified a key carotenoid metabolism enzyme,...

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“…In addition to the simple diffusion mechanism for cellular uptake of carotenoids into intestinal epithelia, scavenger receptor class B type 1(SR-B1) and cluster determinant 36 (CD36) have recently been found to mediate facilitated diffusion, based on in vitro study using human intestinal cells (Borel et al 2013, During et al 2005. The in vivo intestinal absorption of β-carotene was lower in SR-B1 knockout mice than wild-type mice fed a high-fat and high-cholesterol diet (van Bennekum et al 2005).…”

Section: Intestinal Absorptionmentioning

confidence: 99%

“…Significant interindividual differences in carotenoid accumulation have been known to occur in human plasma. Recently, it was suggested that plasma levels of several provitamin A carotenoids are associated with genotypes of genes encoding SR-B1 and CD36 in human subjects (Borel et al 2013). Genetic variants of the proteins that mediate facilitated diffusion might be partly responsible for interindividual differences in carotenoid bioavailability.…”

Section: Intestinal Absorptionmentioning

confidence: 99%

Bioavailability of Dietary Carotenoids: Intestinal Absorption and Metabolism

Nagao

2014

JARQ

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CD36 and SR-BI Are Involved in Cellular Uptake of Provitamin A Carotenoids by Caco-2 and HEK Cells, and Some of Their Genetic Variants Are Associated with Plasma Concentrations of These Micronutrients in Humans

Cited by 121 publications

References 43 publications

β-Carotene Increases Muscle Mass and Hypertrophy in the Soleus Muscle in Mice

β-Carotene Increases Muscle Mass and Hypertrophy in the Soleus Muscle in Mice

High-density lipoprotein receptor SCARB1 is required for carotenoid coloration in birds

Bioavailability of Dietary Carotenoids: Intestinal Absorption and Metabolism

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CD36 and SR-BI Are Involved in Cellular Uptake of Provitamin A Carotenoids by Caco-2 and HEK Cells, and Some of Their Genetic Variants Are Associated with Plasma Concentrations of These Micronutrients in Humans

Cited by 121 publications

References 43 publications

&beta;-Carotene Increases Muscle Mass and Hypertrophy in the Soleus Muscle in Mice

&beta;-Carotene Increases Muscle Mass and Hypertrophy in the Soleus Muscle in Mice

High-density lipoprotein receptor SCARB1 is required for carotenoid coloration in birds

Bioavailability of Dietary Carotenoids: Intestinal Absorption and Metabolism

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β-Carotene Increases Muscle Mass and Hypertrophy in the Soleus Muscle in Mice

β-Carotene Increases Muscle Mass and Hypertrophy in the Soleus Muscle in Mice