CD34 is a specific marker of mature murine mast cells

Drew, Erin; Merkens, Helen; Chelliah, Shierley; Doyonnas, Régis; McNagny, Kelly M.

doi:10.1016/s0301-472x(02)00890-1

Cited by 75 publications

(68 citation statements)

References 63 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…45,46 However, the CD34 variant present on activated hematopoietic stem cells (as circulating precursor cells) may help in their migration and recruitment to other microenvironments. [47][48][49] In our study, we showed that circulating precursor cells have the capacity to become epithelial-like cells by reducing the expression of CD34 and gaining the presence of epithelial markers such as 14-3-3 and keratin proteins.…”

Section: Discussionmentioning

confidence: 63%

Transdifferentiation of Peripheral Blood Mononuclear Cells into Epithelial-Like Cells

Medina

Kilani

Carr

et al. 2007

The American Journal of Pathology

View full text Add to dashboard Cite

Bone marrow-derived stem cells have the potential to transdifferentiate into unexpected peripheral cells. We hypothesize that circulating bone marrow-derived stem cells might have the capacity to transdifferentiate into epithelial-like cells and release matrix metalloproteinase-1-modulating factors such as 14-3-3 for dermal fibroblasts. We have characterized a subset of peripheral blood mononuclear cells (PBMCs) that develops an epithelial-like profile. Our findings show that these cells develop epithelial-like morphology and express 14-3-3 and keratin-5, -8 as early as day 7 and day 21, respectively. When compared with control, conditioned media collected from PBMCs in advanced epithelial-like differentiation (cultures on days 28, 35, and 42) increased the matrix metalloproteinase-1 expression in dermal fibroblasts (P < 0.01). The depletion of 14-3-3 from these conditioned media by immunoprecipitation reduced the effect by 39.5% (P value, 0.05). Therefore, the releasable 14-3-3 from PBMC-derived epithelial-like cells is involved in this process. Our findings provide new insights into the PBMC transdifferentiation to generate epithelial-like cells and subsequently release of 14-3-3 that will disclose new therapeutic alternatives for different dermal clinical settings. (Am J Pathol

show abstract

Section: Discussionmentioning

confidence: 63%

Transdifferentiation of Peripheral Blood Mononuclear Cells into Epithelial-Like Cells

Medina

Kilani

Carr

et al. 2007

The American Journal of Pathology

View full text Add to dashboard Cite

show abstract

“…Importantly, we have previously shown that MC and their precursors are extremely difficult to distinguish from the earliest hematopoietic progenitors (52). In light of our observation that mature MC (i) express high levels of CD34 (24,52), (ii) are well known sources of MMP2 and MMP9 (53,54), and (iii) express CCR1 and chemotax in response to CCL9 (reviewed in ref. 55), it is likely that these iMC in fact represent mature MC, degranulated MC, or MCp.…”

Section: Discussionmentioning

confidence: 91%

Mast cells are an essential hematopoietic component for polyp development

Gounaris

Erdman

Restaino

et al. 2007

Proc. Natl. Acad. Sci. U.S.A.

224

252

View full text Add to dashboard Cite

It is generally agreed that most colon cancers develop from adenomatous polyps, and it is this fact on which screening strategies are based. Although there is overwhelming evidence to link intrinsic genetic lesions with the formation of these preneoplastic lesions, recent data suggest that the tumor stromal environment also plays an essential role in this disease. In particular, it has been suggested that CD34 ؉ immature myeloid precursor cells are required for tumor development and invasion. Here we have used mice conditional for the stabilization of ␤-catenin or defective for the adenomatous polyposis coli (APC) gene to reinvestigated the identity and importance of tumor-infiltrating hematopoietic cells in polyposis. We show that, from the onset, polyps are infiltrated with proinflammatory mast cells (MC) and their precursors. Depletion of MC either pharmacologically or through the generation of chimeric mice with genetic lesions in MC development leads to a profound remission of existing polyps. Our data suggest that MC are an essential hematopoietic component for preneoplastic polyp development and are a novel target for therapeutic intervention.cancer ͉ inflammation ͉ polyposis ͉ TNF␣

show abstract

“…CD34 is a stem cell surface sialomucin and we have shown this molecule is a relatively selective marker of mature mast cells in mice and is required for efficient trafficking in vivo (13). Thus, we have found that, although Cd34 Ϫ/Ϫ mice have normal peripheral tissue mast cell numbers at steady-state, they have a profound defect in mast cell mobilization and recruitment during inflammation of mucosal sites (4,14).…”

mentioning

confidence: 78%

“…Because CD34 is expressed by endothelial cells, microglia, mast cells, and eosinophils (13,(25)(26)(27), the loss of CD34 from any one of these cell types could account for the enhanced disease. We therefore focused our attention on whether CD34 expression on mast cells specifically modulates EAE severity.…”

Section: Discussionmentioning

confidence: 99%

Bone Marrow-Derived Mast Cells Accumulate in the Central Nervous System During Inflammation but Are Dispensable for Experimental Autoimmune Encephalomyelitis Pathogenesis

Bennett

Blanchet

Zhao

et al. 2009

The Journal of Immunology

View full text Add to dashboard Cite

Reports showing that W/Wv mice are protected from experimental autoimmune encephalomyelitis (EAE, a murine model of multiple sclerosis), have implicated mast cells as an essential component in disease susceptibility, but the role of mast cell trafficking has not been addressed. In this study, we have used both mast cell transplantation and genetic mutations (Cd34−/−, W/Wv, Wsh/Wsh) to investigate the role of mast cell trafficking in EAE in detail. We show, for the first time, that bone marrow-derived mast cells are actively recruited to the CNS during EAE. Unexpectedly, however, we found that EAE develops unabated in two independent genetic backgrounds in the complete absence of mast cells or bone marrow-derived mast cell reconstitution. We conclude that although mast cells do accumulate in the brain and CNS during demyelinating disease via peripheral mast cell trafficking, they are completely dispensable for development of disease.

show abstract

CD34 is a specific marker of mature murine mast cells

Cited by 75 publications

References 63 publications

Transdifferentiation of Peripheral Blood Mononuclear Cells into Epithelial-Like Cells

Transdifferentiation of Peripheral Blood Mononuclear Cells into Epithelial-Like Cells

Mast cells are an essential hematopoietic component for polyp development

Bone Marrow-Derived Mast Cells Accumulate in the Central Nervous System During Inflammation but Are Dispensable for Experimental Autoimmune Encephalomyelitis Pathogenesis

Contact Info

Product

Resources

About