CD34+ cell infusion after ST elevation myocardial infarction is associated with improved perfusion and is dose dependent

Quyyumi, Arshed A.; Waller, Edmund K.; Murrow, Jonathan R.; Esteves, Fábio; Galt, James R.; Oshinski, John N.; Lerakis, Stamatios; Sher, Salman; Vaughan, Douglas E.; Perin, Emerson C.; Willerson, James T.; Kereiakes, Dean J.; Gersh, Bernard J.; Gregory, Douglas; Werner, Astrid; Moss, Thomas J.; Chan, Woon‐Khiong; Preti, Robert A.; Pecora, Andrew L.

doi:10.1016/j.ahj.2010.09.025

Cited by 143 publications

(113 citation statements)

References 26 publications

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“…Our findings are consistent with the dose-dependent functional improvements observed in the clinic following intracoronary infusion of autologous CD34 + cells in AMI patients [23]. Circulating human CD34 + cells also contain a small fraction of endothelial progenitor cells (EPC) [33].…”

Section: Discussionsupporting

confidence: 78%

“…Mobilized CD34 + cells have been shown to contribute to vasculogenesis and cardiomyogenesis in rats [21], and have also been shown to have greater efficacy than total mononuclear cells [22]. Autologous CD34 + cells infused into the infarcted artery of acute MI patients in human trials showed a similar positive response to the findings in rat models [23], although this was not carried out in direct comparison to the total BM cell population. A 2012 meta-analysis of randomized controlled clinical trials concluded intracoronary delivery of BM cells significantly increased functionality and reduced MI reoccurrence [24].…”

Section: Introductionmentioning

confidence: 51%

“…There has been controversy over the possible efficacy and mechanism of BM treatment of MI focused around the persistence and function of stem cells in the myocardium [7,8,35], with findings both dismissing and supporting the trans-differentiation of non-cardiac lineage stem cells into functional cardiomyocytes and endothelial tissue. Despite these contradictions, there is a growing body of data from studies of bone marrow derived cell transplantation post-MI in animal models [9,[36][37][38] and human clinical trials [2,14,23,[39][40][41][42] that show improvements in heart function over the short and long term consistent enough to be retained in extensive meta-analysis [24]. The variability in reported data highlight the need to better understand what makes some cellular therapies successful whilst others effect no improvement.…”

Section: Discussionmentioning

confidence: 99%

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Hematopoietic stem cells are a critical sub-population of whole bone marrow in the treatment of myocardial infarction

Davy¹,

Walker²,

Wong³

et al. 2013

SCD

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Recent studies suggest that whole bone marrow (WBM) derived stem cells may facilitate recovery following myocardial infarction. However, the sub-population of WBM responsible for recovery remains uncertain. By adjusting the abundance of CD34 + LinNeg cells in human bone marrow we examined the relative significance of hematopoietic stem cells (HSC) in the recovery of cardiac function in a murine model of induced myocardial infarction. Enrichment of HSC by ~100-fold in WBM transplanted into mice significantly increased recovery of heart function and reduced scar size compared to transplantation of WBM depleted in HSC by ~10-fold (P < 0.05, and P < 0.01 respectively). Peri-infarct capillary density was significantly increased in recipients of HSC-enriched samples (P < 0.01) or WBM samples (P < 0.01) compared to controls. These results strongly suggest a critical role for HSC in the effective treatment of myocardial infarction with human bone marrow, and imply that enrichment of HSC may markedly benefit the clinical application of WBM treatments.

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Section: Discussionsupporting

confidence: 78%

Section: Introductionmentioning

confidence: 51%

Section: Discussionmentioning

confidence: 99%

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Hematopoietic stem cells are a critical sub-population of whole bone marrow in the treatment of myocardial infarction

Davy¹,

Walker²,

Wong³

et al. 2013

SCD

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“…Central to the development of the technique was the identification of transmembrane proteins that selectively define a target cell population. For example, isolation of CD34 + and Thy1 + cells facilitates purification of hematopoietic stem cells that can reconstitute the hematopoietic system as part of treatment for cancer patients (7)(8)(9)(10). A current obstacle for the development of similar techniques for the purification of specific neural progenitor phenotypes, including mDA progenitors, is a lack of knowledge regarding the identity of suitable transmembrane targets.…”

mentioning

confidence: 99%

Transcriptome analysis reveals transmembrane targets on transplantable midbrain dopamine progenitors

Bye

Jönsson

Björklund

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

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An important challenge for the continued development of cell therapy for Parkinson's disease (PD) is the establishment of procedures that better standardize cell preparations for use in transplantation. Although cell sorting has been an anticipated strategy, its application has been limited by lack of knowledge regarding transmembrane proteins that can be used to target and isolate progenitors for midbrain dopamine (mDA) neurons. We used a "FACS-array" approach to identify 18 genes for transmembrane proteins with high expression in mDA progenitors and describe the utility of four of these targets (Alcam, Chl1, Gfra1, and Igsf8) for isolating mDA progenitors from rat primary ventral mesencephalon through flow cytometry. Alcam and Chl1 facilitated a significant enrichment of mDA neurons following transplantation, while targeting of Gfra1 allowed for robust separation of dopamine and serotonin neurons. Importantly, we also show that mDA progenitors isolated on the basis of transmembrane proteins are capable of extensive, functional innervation of the host striatum and correction of motor impairment in a unilateral model of PD. These results are highly relevant for current efforts to establish safe and effective stem cell-based procedures for PD, where clinical translation will almost certainly require safety and standardization measures in order to deliver well-characterized cell preparations.Parkinson's disease | cell sorting | Alcam | microarray | transplantation

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“…1 endothelial progenitor cells in the blood represents a novel method to predict clinical outcomes in patients with peripheral arterial and cardiovascular disease (15,16).…”

Section: Cation Of Extremely Rare Cd34mentioning

confidence: 99%

Translational Applications of Flow Cytometry in Clinical Practice

Jaye

Bray

Gebel

et al. 2012

The Journal of Immunology

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100

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Flow cytometry has evolved over the past 30 y from a niche laboratory technique to a routine tool used by clinical pathologists and immunologists for diagnosis and monitoring of patients with cancer and immune deficiencies. Identification of novel patterns of expressed Ags has led to the recognition of cancers with unique pathophysiologies and treatment strategies. FACS had permitted the isolation of tumor-free populations of hematopoietic stem cells for cancer patients undergoing stem cell transplantation. Adaptation of flow cytometry to the analysis of multiplex arrays of fluorescent beads that selectively capture proteins and specific DNA sequences has produced highly sensitive and rapid methods for high through-put analysis of cytokines, Abs, and HLA genotypes. Automated data analysis has contributed to the development of a “cytomics” field that integrates cellular physiology, genomics, and proteomics. In this article, we review the impact of the flow cytometer in these areas of medical practice.

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CD34+ cell infusion after ST elevation myocardial infarction is associated with improved perfusion and is dose dependent

Cited by 143 publications

References 26 publications

Hematopoietic stem cells are a critical sub-population of whole bone marrow in the treatment of myocardial infarction

Hematopoietic stem cells are a critical sub-population of whole bone marrow in the treatment of myocardial infarction

Transcriptome analysis reveals transmembrane targets on transplantable midbrain dopamine progenitors

Translational Applications of Flow Cytometry in Clinical Practice

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