CD34+CDw90(Thy-1)+ subset colocated with mesenchymal progenitors in human normal bone marrow hematon units is enriched in colony-forming unit megakaryocytes and long-term culture-initiating cells

Boiret, Nathalie; Rapatel, Chantal; Boisgard, Stéphane; Charrier, Sabine; Tchirkov, Andréï; Bresson, Caroline; Camilleri, Lionel; Berger, Juliette; Guy, Laurent; Guérin, Jean-Jacques; Pigeon, Pascale; Chassagne, J; Berger, Marc

doi:10.1016/j.exphem.2003.08.010

Cited by 19 publications

(30 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It is generally accepted that BM-MSCs are a highly adhesive fibroblast-like cell type. Our previous results [16] jointly with data from others [17] indicate the existence of a population of non-adherent, small, round cells, with self-renewal and multilineage differentiation potential in adult bone marrow, and those cells are capable of forming CFU-Fs in vitro . Accordingly we have developed the “Pour-off” method to amplify the 1 st BM-MSC.…”

Section: Introductionsupporting

confidence: 70%

An Improved Transplantation Strategy for Mouse Mesenchymal Stem Cells in an Acute Myocardial Infarction Model

Jin

Zhao²,

Tan³

et al. 2011

PLoS ONE

View full text Add to dashboard Cite

To develop an effective therapeutic strategy for cardiac regeneration using bone marrow mesenchymal stem cells (BM-MSCs), the primary mouse BM-MSCs (1st BM-MSCs) and 5th passage BM-MSCs from β-galactosidase transgenic mice were respectively intramyocardially transplanted into the acute myocardial infarction (AMI) model of wild type mice. At the 6th week, animals/tissues from the 1st BM-MSCs group, the 5th passage BM-MSCs group, control group were examined. Our results revealed that, compared to the 5th passage BM-MSCs, the 1st BM-MSCs had better therapeutic effects in the mouse MI model. The 1st BM-MSCs maintained greater differentiation potentials towards cardiomocytes or vascular endothelial cells in vitro. This is indicated by higher expressions of cardiomyocyte and vascular endothelial cell mature markers in vitro. Furthermore, we identified that 24 proteins were down-regulated and 3 proteins were up-regulated in the 5th BM-MSCs in comparison to the 1st BM-MSCs, using mass spectrometry following two-dimensional electrophoresis. Our data suggest that transplantation of the 1st BM-MSCs may be an effective therapeutic strategy for cardiac tissue regeneration following AMI, and altered protein expression profiles between the 1st BM-MSCs and 5th passage BM-MSCs may account for the difference in their maintenance of stemness and their therapeutic effects following AMI.

show abstract

Section: Introductionsupporting

confidence: 70%

An Improved Transplantation Strategy for Mouse Mesenchymal Stem Cells in an Acute Myocardial Infarction Model

Jin

Zhao²,

Tan³

et al. 2011

PLoS ONE

View full text Add to dashboard Cite

show abstract

“…38 In our study, cell surface marker expression of BRCs from the subject who underwent cell transplantation in the bone defect showed 65-fold and 5-fold increases in CD90þ and CD105þ, respectively (data not shown). This served as an indication that the BRC product was highly enriched with cells possessing mesenchymal and angiogenic potential and is in accordance with previous reports demonstrating enrichment of theses cell types with this cell-processing protocol.…”

Section: Discussionmentioning

confidence: 97%

Angiogenic and Osteogenic Potential of Bone Repair Cells for Craniofacial Regeneration

Kaigler

Pagni

Park

et al. 2010

Tissue Engineering Part A

View full text Add to dashboard Cite

There has been increased interest in the therapeutic potential of bone marrow derived cells for tissue engineering applications. Bone repair cells (BRCs) represent a unique cell population generated via an ex vivo, closed-system, automated cell expansion process, to drive the propagation of highly osteogenic and angiogenic cells for bone engineering applications. The aims of this study were (1) to evaluate the in vitro osteogenic and angiogenic potential of BRCs, and (2) to evaluate the bone and vascular regenerative potential of BRCs in a craniofacial clinical application. BRCs were produced from bone marrow aspirates and their phenotypes and multipotent potential characterized. Flow cytometry demonstrated that BRCs were enriched for mesenchymal and vascular phenotypes. Alkaline phosphatase and von Kossa staining were performed to assess osteogenic differentiation, and reverse transcriptase-polymerase chain reaction was used to determine the expression levels of bone specific factors. Angiogenic differentiation was determined through in vitro formation of tube-like structures and fluorescent labeling of endothelial cells. Finally, 6 weeks after BRC transplantation into a human jawbone defect, a biopsy of the regenerated site revealed highly vascularized, mineralized bone tissue formation. Taken together, these data provide evidence for the multilineage and clinical potential of BRCs for craniofacial regeneration.

show abstract

“…[9][10][11][12][13][14] Several markers have been proposed for use in identification and positive selection of osteogenic cells from bone marrow, including STRO-1, 15 CD271, 16 CD49a, 17 CD146, 18 and CD105. 19 We have recently reported that the presence of hyaluronan (HA; also known as hyaluronic acid or hyaluronate) on the surface of freshly isolated marrow-derived cells can also be used as a marker for positive selection. 20 HA, is a common, but highly regulated component of extracellular matrix.…”

mentioning

confidence: 99%

In VivoTransplantation of Autogenous Marrow-Derived Cells Following Rapid Intraoperative Magnetic Separation Based on Hyaluronan to Augment Bone Regeneration

Caralla

Joshi

Fleury

et al. 2013

Tissue Engineering Part A

View full text Add to dashboard Cite

CD34+CDw90(Thy-1)+ subset colocated with mesenchymal progenitors in human normal bone marrow hematon units is enriched in colony-forming unit megakaryocytes and long-term culture-initiating cells

Cited by 19 publications

References 43 publications

An Improved Transplantation Strategy for Mouse Mesenchymal Stem Cells in an Acute Myocardial Infarction Model

An Improved Transplantation Strategy for Mouse Mesenchymal Stem Cells in an Acute Myocardial Infarction Model

Angiogenic and Osteogenic Potential of Bone Repair Cells for Craniofacial Regeneration

In VivoTransplantation of Autogenous Marrow-Derived Cells Following Rapid Intraoperative Magnetic Separation Based on Hyaluronan to Augment Bone Regeneration

Contact Info

Product

Resources

About