CD30 triggering by agonistic antibodies regulates CXCR4 expression and CXCL12 chemotactic activity in the cell line L540

Abstract

IntroductionThe tumor necrosis factor receptor (TNFR) family includes molecules such as TNFR1, TNFR2, CD27, CD30, CD40, CD95, and OX40, which interact with a corresponding family of TNF-like cytokines 1,2 integrating signals of activation, proliferation, apoptosis, or differentiation, depending on cell type, specific receptor expression, coactivation signals, and mobilization of transduction molecules. 1-4 CD30, CD40, OX40, and CD95 at least have been involved in lymphoid differentiation and effector functions. 5 CD30, originally described as a marker of a number of lymphoma cells and reactive lymphoblasts, 6 is expressed by activated and memory T cells, 6-9 medullary thymocytes, 10,11 B cells, natural killer cells, and some nonhematologic cells. 12 Its expression by T cells is essentially dependent on activation involving IL-4 and CD28 signaling. 9,13 The IL-4 requirement is probably the reason for the association of CD30 expression with preferential Th0-and Th2-type immune responses in vitro 8 and in vivo. [14][15][16] Stimulation of CD30 by CD153 (CD30 ligand) leads to nuclear mobilization of NF-B complexes 17 while inducing a signal-coupled depletion of TRAF2, which increases the cell sensitivity to TNFR1-dependent apoptosis. 3 This dual signaling may explain the pleiotropic effects after CD30 stimulation in CD30 ϩ lymphoma cell lines 18 and T cells, the latter acquiring a Th2-type function, 19 associated with inhibitory functions, 20 based on impaired clonal expansion of T cells. 10,20,21 High levels of soluble CD30 or TNF-␣ in the sera of patients infected with human immunodeficiency virus (HIV-1) at diagnosis have been shown to be an independent prognostic indicator of disease progression, 22,23 and the suggested role of CD30 in HIV-1 infection 15 has been linked to its ability to promote HIV-1 shedding through NF-B activation in infected cells. 24 The role of CD30 in HIV-1 infection, 15,22 the preferential association of CD30 with compartmentalized lymphoid subsets during differentiation 11 and effector responses, 16 and the evidence that Th1 versus Th2 or naive versus primed T cells are distinguished by specific patterns of CD30 8 and chemokine receptor expression 25,26 suggest some kind of relationship between CD30 signals and chemokine function. Recently, Muta et al 27 reported that CD30 signals increased amounts of CCR7 mRNA in the YT lymphoma cell line. Actually, a number of CD30-related activities seem to integrate cellular functions driven by lymphoid chemokines, namely their prototypic member CXCL12 (SDF-1) and its receptor CXCR4 (CD184). 28 CXCL12 is expressed constitutively in various cell types [28][29][30] and is an efficient cell chemoattractant to specific sites. [30][31][32] CXCR4 is expressed by neutrophils, monocytes, naive T lymphocytes, thymocytes, mature and immature B cells, CD34 ϩ cells, 31,32 vascular endothelial cells, 33 and neurologic cells. 32 It has been implicated in platelet formation 34 and is a coreceptor for HIV-1 entry. 35 CD30 and CD153 are reasonable candidates for reg...

CD30 ligation differentially affects CXCR4‐dependent HIV‐1 replication and soluble CD30 secretion in non‐Hodgkin cell lines and in γ δ T lymphocytes

“…One stimulus for release of sCD30 is the interaction itself between the ligand, CD30L/CD153, and CD30, as recently documented in both the ALCL Karpas 299 and in the Hodgkin-derived L540 cell lines [39,42]. Our study reinforces and extends this finding to a panel of nonHodgkin T lymphocytic and a subset of promonocytic cells, suggesting that this may be considered a general mechanism occurring in CD30 + neoplastic cell lines.…”

“…CD30 triggering up-regulates CXCR4 expression in the Hodgkin-derived cell line L540 [39]. Since CXCR4 is a coreceptor that affects entry of X4 HIV, and its expression is up-regulated by the Th2-produced cytokine IL-4 [40], an IL-4-mediated autocrine loop enhances HIV replication in Th2 cells [41].…”

Lymphoma microenvironment: culprit or innocent?

“…[84][85][86][87][88] Recruitment of TRAF proteins to the cytoplasmic tail of the receptor transduces signals to activate IKK, which phosphorylates IkBa and triggers NFkB translocation into the nucleus. 89 New data, which require confirmation, suggest that CD30 can trigger upregulation of CXCR4 in non-Hodgkin lymphoma (NHL) cell lines, T lymphocytes, and in one HL cell line, 90,91 providing a new link between CD30-expressing lymphomas and their microenvironment.…”

HLXB9 activates IL6 in Hodgkin lymphoma cell lines and is regulated by PI3K signalling involving E2F3

“…This implies an antiapoptotic role for IL6 in HL cells consistent with previous data concerning IL6 in this context. 12 L-540 cells express MCL1, though lacking both NFkB activity 31 and HLXB9 expression (Figure 1, Supplementary Table 2). However, L-540 exhibits very strong constitutive STAT3 activity, 12 which may substitute the active autocrine IL6 signalling pathways present in the other HL cell lines.…”

“…12 Both NFkB and AP-1 are constitutively active in most HL cells 5,6 or inducible by TPA in the remainder. 31 Thus, TPA treatment of KM-H2 with low levels of constitutive AP-1 activity, 6 and of L-540, which lacks constitutive NFkB activity, 31 respectively, elevated or induced expression of IL6 therein (Table 1). These results confirm that expression of IL6 depends on AP-1 and NFkB activation in the HL cell lines used in this study.…”