CD3<sup>-</sup> Leukocytes Present in the Human Uterus During Early Placentation: Phenotypic and MorphologicCharacterization of the CD56<sup>++</sup> Population

King, Ashley; Balendran, N; Wooding, Peter; Carter, N. P.; Loke, Y.W.

doi:10.1155/1991/83493

Cited by 265 publications

(186 citation statements)

References 23 publications

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“…The latter strikingly distinguishes mouse from human uNK cells, since human CD56 bright uNK cells are CD16 Ϫ (22,23). Mouse peripheral NK cells can be subdived in functional subsets by virtue of CD27 and CD11b expression (24).…”

Section: Have a Unique Phenotypementioning

confidence: 99%

Unique Receptor Repertoire in Mouse Uterine NK cells

Yadi

Burke

Madeja

et al. 2008

The Journal of Immunology

131

153

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Uterine NK (uNK) cells are a prominent feature of the uterine mucosa and regulate placentation. NK cell activity is regulated by a balance of activating and inhibitory receptors, however the receptor repertoire of mouse uNK cells is unknown. We describe herein two distinct subsets of CD3−CD122+ NK cells in the mouse uterus (comprising decidua and mesometrial lymphoid aggregate of pregnancy) at mid-gestation: a small subset indistinguishable from peripheral NK cells, and a larger subset that expresses NKp46 and Ly49 receptors, but not NK1.1 or DX5. This larger subset reacts with Dolichus biflores agglutinin, a marker of uNK cells in the mouse, and is adjacent to the invading trophoblast. By multiparametric analysis we show that the phenotype of uNK cells is unique and unprecedented in terms of adhesion, activation, and MHC binding potential. Thus, the Ly49 repertoire and the expression of other differentiation markers strikingly distinguish uNK cells from peripheral NK cells, suggesting that a selection process shapes the receptor repertoire of mouse uNK cells.

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Section: Have a Unique Phenotypementioning

confidence: 99%

Unique Receptor Repertoire in Mouse Uterine NK cells

Yadi

Burke

Madeja

et al. 2008

The Journal of Immunology

131

153

View full text Add to dashboard Cite

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“…Moreover, high numbers of CD56 ϩ large granular lymphocytes (LGL) are present in the decidua during early pregnancy; yet, even so, the trophoblast is usually not destroyed. Up to 70% of all decidual lymphocytes show this phenotype (35). Based on the two NK receptor theory (25), there are two possible explanations for nonrecognition of the fetal cytotrophoblast by maternal NK cells.…”

Section: Discussionmentioning

confidence: 99%

Human histocompatibility leukocyte antigen (HLA)-G molecules inhibit NKAT3 expressing natural killer cells

1997

Journal of Reproductive Immunology

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SummaryThe crucial immunological function of the classical human major histocompatibility complex (MHC) class I molecules, human histocompatibility leukocyte antigen (HLA)-A, -B, and -C, is the presentation of peptides to T cells. A secondary function is the inhibition of natural killer (NK) cells, mediated by binding of class I molecules to NK receptors. In contrast, the function of the nonclassical human MHC class I molecules, HLA-E, -F, and -G, is still a mystery. The specific expression of HLA-G in placental trophoblast suggests an important role for this molecule in the immunological interaction between mother and child. The fetus, semiallograft by its genotype, escapes maternal allorecognition by downregulation of HLA-A and HLA-B molecules at this interface. It has been suggested that the maternal NK recognition of this downregulation is balanced by the expression of HLA-G, thus preventing damage to the placenta. Here, we describe the partial inhibition of NK lysis of the MHC class I negative cell line LCL721.221 upon HLA-G transfection. We present three NK lines that are inhibited via the interaction of their NKAT3 receptor with HLA-G and with HLA-Bw4 molecules. Inhibition can be blocked by the anti-NKAT3 antibody 5.133. In conclusion, NK inhibition by HLA-G via NKAT3 may contribute to the survival of the fetal semiallograft in the mother during pregnancy.

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“…Üç renkli akım sitometride 488nm de 15 mW argon lazer kullanıldı. B Bu ul lg gu ul la ar r: : Ortalama gebelik haftaları; spontan düşük grubunda 8,3 (6)(7)(8)(9)(10)(11), normal gebelik grubunda ise 7,7 (5-10) olarak saptandı. CD56 + NK hücre yüzdesi normal gebelik grubunda %51,1 (34,5-65,2) spontan düşük grubunda ise %53,3 (32,1-72,2) olarak saptandı p>0,05 A AB BS S T TR RA AC CT T O Ob bj je ec ct ti iv ve e: : To evaluate the decidual Natural Killer (NK) cell percentage with of the elective early pregnancy terminations with spontaneous abortions flow cytometry analysis.…”

Section: ö öZ Ze Et T Aunclassified

“…Three-colourflow cytometry utilized an argon laser with 15 mW at 488 nm excitation. R Re es su ul lt ts s: : Median gestation ages were 8.3 (6)(7)(8)(9)(10)(11) 2 Tekrarlayan gebelik kayıpla-rının %20-50'sinin immünolojik nedenlere bağlı olduğu düşünülmektedir. 3 Bu durum dikkatleri immün sistem üzerine çekmiştir.…”

Section: ö öZ Ze Et T Aunclassified

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Role of Uterine Natural Killer Cells in Early Pregnancy Loss

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2015

Turkiye Klinikleri J Gynecol Obst

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CD3^- Leukocytes Present in the Human Uterus During Early Placentation: Phenotypic and MorphologicCharacterization of the CD56⁺⁺ Population

Cited by 265 publications

References 23 publications

Unique Receptor Repertoire in Mouse Uterine NK cells

Unique Receptor Repertoire in Mouse Uterine NK cells

Human histocompatibility leukocyte antigen (HLA)-G molecules inhibit NKAT3 expressing natural killer cells

Role of Uterine Natural Killer Cells in Early Pregnancy Loss

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CD3- Leukocytes Present in the Human Uterus During Early Placentation: Phenotypic and MorphologicCharacterization of the CD56++ Population

Cited by 265 publications

References 23 publications

Unique Receptor Repertoire in Mouse Uterine NK cells

Unique Receptor Repertoire in Mouse Uterine NK cells

Human histocompatibility leukocyte antigen (HLA)-G molecules inhibit NKAT3 expressing natural killer cells

Role of Uterine Natural Killer Cells in Early Pregnancy Loss

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Product

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CD3^- Leukocytes Present in the Human Uterus During Early Placentation: Phenotypic and MorphologicCharacterization of the CD56⁺⁺ Population