Cd271 mediates proliferation and differentiation of epidermal stem cells to support cutaneous burn wound healing

Zhang, Min; Cao, Yongqian; Li, Xiaohong; Hu, Lizhi; Taieb, Sahbi Khaled; Zhu, Xiaolong; Zhang, Jing; Feng, Yongqiang; Zhao, Ran; Wang, Mingqing; Xue, Wenrui; Yang, Zhanjie; Wang, Yibing

doi:10.1007/s00441-017-2723-8

Cited by 18 publications

(7 citation statements)

References 32 publications

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“…Another experiment held on murine epidermal stem cell culture revealed, that overexpression of Cd271 (p75 neurotrophin receptor) increased cell ability to differentiate, proliferate and migrate. The same study also proved, that Cd271 promotes burn wound healing on mice model (Zhang et al, 2018a). Human stem cells have also been subject to some genetic modifications.…”

Section: Genetic Modifications Of Stem Cellsmentioning

confidence: 64%

Novel trends in application of stem cells in skin wound healing

Kucharzewski

Rojczyk

Wilemska-Kucharzewska

et al. 2019

European Journal of Pharmacology

163

119

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The latest findings indicate the huge therapeutic potential of stem cells in regenerative medicine, including the healing of chronic wounds. Main stem cell types involved in wound healing process are: epidermal and dermal stem cells, mesenchymal stem cells (MSCs), endothelial progenitor cells (EPCs) and hematopoietic stem cells (HSCs). In the therapy of chronic wounds, they can be administrated either topically or using different matrix like hydrogels, scaffolds, dermal substitutes and extracellular matrix (ECM) derivatives. Stem cells are proven to positively influence wound healing by different direct and indirect mechanisms including residing cells stimulation, biomolecules release, inflammation control and ECM remodelling. MSCs are especially worth mentioning as they can be easily derived from bone-marrow or adipose tissue. Apart from traditional approach of administering living stem cells to wounds, new trends have emerged in recent years. Good healing results are obtained using stem cell secretome alone, for example exosomes or conditioned media. There are also attempts to improve healing potential of stem cells by their co-culture with other cell types as well as by their genetic modifications or pretreatment using different chemicals or cell media. Moreover, stem cells have been tested for novel therapeutic purposes like for example acute burns and have been used in experiments on large animal models including pigs and sheep. In this review we discuss the role of stem cells in skin wound healing acceleration. In addition, we analyse possible new strategies of stem cells application in treatment of chronic wounds.

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Section: Genetic Modifications Of Stem Cellsmentioning

confidence: 64%

Novel trends in application of stem cells in skin wound healing

Kucharzewski

Rojczyk

Wilemska-Kucharzewska

et al. 2019

European Journal of Pharmacology

163

119

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“…The other half of the above tissue was fixed in 10% formalin solution, embedded in paraffin and sectioned at 3 μm for histochemical analyses which were performed by haematoxylin–eosin (H&E) staining and immunohistochemical (IHC) staining. Deparaffinization, rehydration, antigen retrieval, endogenous peroxidase blocking, and goat serum (#SP-9001; ZSGB-BIO, Beijing, China) blocking of paraffin sections were performed as previously described [ 33 – 35 ]. Next, the wound skin tissue sections were incubated with primary antibody against RAMP1 (#ab156575; Abcam Plc, Cambridge, UK) at a dilution of 1:200 at 4 °C overnight.…”

Section: Methodsmentioning

confidence: 99%

Receptor activity‐modifying protein 1 regulates mouse skin fibroblast proliferation via the Gαi3-PKA-CREB-YAP axis

Yin

Song

et al. 2022

Cell Commun Signal

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Background Skin innervation is crucial for normal wound healing. However, the relationship between nerve receptors and wound healing and the intrinsic mechanism remains to be further identified. In this study, we investigated the role of a calcitonin gene-related peptide (CGRP) receptor component, receptor activity‐modifying protein 1 (RAMP1), in mouse skin fibroblast (MSF) proliferation. Methods In vivo, Western blotting and immunohistochemical (IHC) staining of mouse skin wounds tissue was used to detect changes in RAMP1 expression. In vitro, RAMP1 was overexpressed in MSF cell lines by infection with Tet-On-Flag-RAMP1 lentivirus and doxycycline (DOX) induction. An IncuCyte S3 Live-Cell Analysis System was used to assess and compare the proliferation rate differences between different treatment groups. Total protein and subcellular extraction Western blot analysis, quantitative real-time-polymerase chain reaction (qPCR) analysis, and immunofluorescence (IF) staining analysis were conducted to detect signalling molecule expression and/or distribution. The CUT & RUN assay and dual-luciferase reporter assay were applied to measure protein-DNA interactions. Results RAMP1 expression levels were altered during skin wound healing in mice. RAMP1 overexpression promoted MSF proliferation. Mechanistically, total Yes-associated protein (YAP) and nuclear YAP protein expression was increased in RAMP1-overexpressing MSFs. RAMP1 overexpression increased inhibitory guanine nucleotide-binding protein (G protein) α subunit 3 (Gαi3) expression and activated downstream protein kinase A (PKA), and both elevated the expression of cyclic adenosine monophosphate (cAMP) response element-binding protein (CREB) and activated it, promoting the transcription of YAP, elevating the total YAP level and promoting MSF proliferation. Conclusions Based on these data, we report, for the first time, that changes in the total RAMP1 levels during wound healing and RAMP1 overexpression alone can promote MSF proliferation via the Gαi3-PKA-CREB-YAP axis, a finding critical for understanding RAMP1 function, suggesting that this pathway is an attractive and accurate nerve target for skin wound treatment.

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“…The researchers investigated the kinetic movement of p75NTR + SSCs in wound healing and found that the protein level of p75NTR in the epidermis was decreased at an early stage but increased promptly in the mid to late stages of the burn wound healing process, suggesting that p75NTR was involved in the re-epithelialization and remodeling of the epidermis in the mid-to-late stages. 119 Moreover, Iwata et al confirmed that p75NTR + SSCs proliferated and migrated to the sites of damage from 3 days after wounding and that the expression of TGFβ2, VEGFα, FGF2, EGF, PDGFB, and TGFβ1 in P75NTR + SSCs was higher than that in p75NTR − SSCs, indicating that the effect of p75NTR + SSCs on wound healing was achieved through not only differentiation but also paracrine action. 120…”

Section: Skin-derived Mscsmentioning

confidence: 98%

Nerve growth factor (NGF) and NGF receptors in mesenchymal stem/stromal cells: Impact on potential therapies

Zha

Tian

et al. 2021

Stem Cells Translational Medicine

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Mesenchymal stem/stromal cells (MSCs) are promising for the treatment of degenerative diseases and traumatic injuries. However, MSC engraftment is not always successful and requires a strong comprehension of the cytokines and their receptors that mediate the biological behaviors of MSCs. The effects of nerve growth factor (NGF) and its two receptors, TrkA and p75NTR, on neural cells are well studied. Increasing evidence shows that NGF, TrkA, and p75NTR are also involved in various aspects of MSC function, including their survival, growth, differentiation, and angiogenesis. The regulatory effect of NGF on MSCs is thought to be achieved mainly through its binding to TrkA. p75NTR, another receptor of NGF, is regarded as a novel surface marker of MSCs. This review provides an overview of advances in understanding the roles of NGF and its receptors in MSCs as well as the effects of MSC-derived NGF on other cell types, which will provide new insight for the optimization of MSC-based therapy.

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Cd271 mediates proliferation and differentiation of epidermal stem cells to support cutaneous burn wound healing

Cited by 18 publications

References 32 publications

Novel trends in application of stem cells in skin wound healing

Novel trends in application of stem cells in skin wound healing

Receptor activity‐modifying protein 1 regulates mouse skin fibroblast proliferation via the Gαi3-PKA-CREB-YAP axis

Nerve growth factor (NGF) and NGF receptors in mesenchymal stem/stromal cells: Impact on potential therapies

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