CD271 as a marker to identify mesenchymal stem cells from diverse sources before culture

Abstract

Mesenchymal stem cells, due to their characteristics are ideal candidates for cellular therapy. Currently, in culture these cells are defined by their adherence to plastic, specific surface antigen expression and multipotent differentiation potential. However, the in vivo identification of mesenchymal stem cells, before culture, is not so well established. Pre-culture identification markers would ensure higher purity than that obtained with selection based on adherence to plastic. Up until now, CD271 has been described as the most specific marker for the characterization and purification of human bone marrow mesenchymal stem cells. This marker has been shown to be specifically expressed by these cells. Thus, CD271 has been proposed as a versatile marker to selectively isolated and expand multipotent mesenchymal stem cells with both immunosuppressive and lymphohematopoietic engraftment-promoting properties. This review focuses on this marker, specifically on identification of mesenchymal stem cells from different tissues. Literature revision suggests that CD271 should not be defined as a universal marker to identify mesenchymal stem cells before culture from different sources. In the case of bone marrow or adipose tissue, CD271 could be considered a quite suitable marker; however this marker seems to be inadequate for the isolation of mesenchymal stem cells from other tissues such as umbilical cord blood or wharton's jelly among others.

Substrate-mediated reprogramming of human fibroblasts into neural crest stem-like cells and their applications in neural repair

“…Literature has indicated that CD271 can be found in neural crest stem cells and is considered as one of neural crest cell markers [47]. Besides, CD271 has been proposed as a marker to identify the isolated mesenchymal stem cells [48]. On the other hand, SOX10 maintained multipotency and inhibited neuronal differentiation, but was permissive for glial differentiation of neural crest stem cells [49].…”

Stem cells and chronic wound healing: state of the art

“…3 CD271 + has also been described as the most specific marker for bone marrow-derived mesenchymal stem cells (BM-MSCs) and adipose-derived stem cells (ASCs). 4 However, consensus has yet to be reached with regard to their antigen expression profile. 5 The inconstant nature of surface marker expression as these cells are removed from their native environment and expanded ex vivo merely worsens the surrounding controversy.…”

The Role of Signaling Pathway on Osteoprogenitor Cell Behavior and Bone Formation

“…Furthermore, HMSC with special cluster of differentiation, such as CD 37 , CD 90 , and CD 40 with little increased expression of CD 14 , CD 34 , CD 45 , and human leucocyte-DR (HLA-DR) can differentiate to osteoblast cells (4). Researchers exploit HMSC from different sources, such as dental tissue, endometria menstrual, blood, peripheral blood, placenta and fetal membrane salivary gland, skin, synovial fluid, foreskin, endometrium amniotic fluid, subamniotic umbilical cord lining membrane, and Wharton jelly (5).…”