“…Formalin- or B+-fixed, paraffin-embedded tissues from diagnostic biopsies of DHL cases and BL control cases were characterized by immunohistochemistry using previously described methods (44) with some or all of the following antibodies, if not already performed at the time of diagnosis: CD20 (L26 epitope, prediluted, Ventana Medical Systems, Tucson, AZ), CD79a (prediluted, Ventana), Pax5 (prediluted, Ventana), Bcl6 (1:10 dilution, Dako, Carpinteria, CA), CD10 (prediluted, Ventana), Bcl2 (clone 124, prediluted, Ventana), Mum1/IRF4 (1:20 dilution, Dako), Ki-67 (clone 30-9, prediluted, Ventana), terminal deoxynucleotidyl transferase (TdT, prediluted, Ventana). One case that lacked Bcl2 protein expression using clone 124 (targeting amino acids 41-54) was subsequently analyzed using clone C-2 (targeting amino acids 1-205) (Santa Cruz Biotechnology, Santa Cruz, CA).…”