CD20-positive T cell leukemia/lymphoma: case report and review of the literature

Yokose, Norio; Ogata, Kíyoyuki; Sugisaki, Yuichi; Mori, Shigeki; Yamada, Takashi; An, Emi; Dan, Kazuo

doi:10.1007/s002770100297

Cited by 36 publications

(5 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Co-expression of both T- and B-cell markers was in agreement with expression patterns of neoplastic T-cells showing positive signals for CD3, CD4, CD5, CD8, CD45, and CD20 and negative signals for other B-cell markers such as CD79a and PAX522. Some specific type of B-cell lymphomas show negative staining for CD2023 while some T-cell lymphomas show positive staining for CD2024. It is suggested that although CD markers are specific to particular cell types but in neoplastic conditions their expression becomes non-specific7.…”

Section: Discussionsupporting

confidence: 77%

Level of PAX5 in differential diagnosis of non-Hodgkin′s lymphoma

et al. 2016

View full text Add to dashboard Cite

Background & objectives:The PAX5, a paired box transcription factor and B-cell activator protein (BSAP), activates B-cell commitment genes and represses non-B-cell lineage genes. About 14 transcript variants of PAX5 have been observed in human. Any alteration in its expression pattern leads to lymphogenesis or associated diseases and carcinogenesis in non-lymphoid tissues. Its mechanisms of function in pathophysiology of non-Hodgkin's lymphoma (NHL) are unclear. This study was intended to explore influence of PAX5 in cascade of NHL pathogenesis and diagnosis.Methods:Samples of 65 patients were evaluated by immunohistochemical staining for cellular localization of PAX5, CD19, CD3, cABL, p53, Ras and Raf and by TUNEL assay, RNA-isolation and reverse transcriptase (RT)-PCR, Western blot analysis, and lactate dehydrogenase (LDH) specific staining.Results:B-cell type NHL patients were positive for PAX5, p53, Ras, CD19, Raf and CD3. All of them showed TUNEL-positive cells. The differential expression pattern of PAX5, CD19, p53, CD3, ZAP70, HIF1α, Ras, Raf and MAPK (mitogen-activated protein kinase) at the levels of transcripts and proteins was observed. The LDH assay showed modulation of LDH4 and LDH5 isoforms in the lymph nodes of NHL patients.Interpretation & conclusions:The histological observations suggested that the patients represent diverse cases of NHL like mature B-cell type, mature T-cell type and high grade diffuse B-cell type NHL. The findings indicate that patients with NHL may also be analyzed for status of PAX5, CD19 and ZAP70, and their transcriptional and post-translational variants for the differential diagnosis of NHL and therapy.

show abstract

Section: Discussionsupporting

confidence: 77%

Level of PAX5 in differential diagnosis of non-Hodgkin′s lymphoma

et al. 2016

View full text Add to dashboard Cite

show abstract

“…Whilst expression of CD20 has been reported in T-cell lymphomas [6], this case is one of only very few published observations of CD20 expression in AITL and is unique in that the CD20 expression was acquired upon relapse. The first case of CD20-positive AITL was reported by Yokose et al as peripheral T-cell lymphoma with clinical characteristics resembling angioimmunoblastic lymphadenopathy [7]. The second report was by Tachibana et al in 2011 [8] with features very similar to the herein presented case, in particular also the acquisition of CD20 expression upon disease progression.…”

Section: Discussionsupporting

confidence: 60%

Relapsed angioimmunoblastic T-cell lymphoma with acquired expression of CD20: a case report and review of the literature

et al. 2013

View full text Add to dashboard Cite

BackgroundAngioimmunoblastic T-cell lymphoma is one of the most common types of peripheral T-cell lymphomas, usually presenting at an older age with an aggressive clinical course. Its characteristic morphological presentation and follicular helper T-cell phenotype help to distinguish it from other T-cell lymphomas.Case presentationWe recently encountered the unique case of a 63-year old patient with relapsed tumour-cell rich angioimmunoblastic T-cell lymphoma, presenting with a “classical” phenotype and, in addition, an acquired, strong, aberrant expression of CD20.“Lineage infidelity” of phenotypic markers is a well-documented phenomenon in lymphomas and leukemias, a circumstance currently still poorly understood and with the potential to bring about erroneous interpretations, causing diagnostic havoc. This case represents one of the few documented angioimmunoblastic T-cell lymphomas with strong CD20 expression. Of interest, CD20 expression was only detected in the recurrent lymphoma and not upon initial diagnosis. The clinical importance of this finding lies in the potential for treatment with an anti-CD20 antibody, for instance Rituximab, in addition to standard chemotherapy protocols for angioimmunoblastic T-cell lymphoma.ConclusionDiagnostic work-up of lymphomas to determine their lineage should therefore consider morphology, pheno- as well as genotypic characteristics, where appropriate, and in particular signs of progression and change in marker profile in relapsed cases e.g. acquisition of “non-lineage” markers such as CD20 in T-cell lymphoma.

show abstract

“…Although rare CD20 + B cells that express CD3 have been reported to exist (Yokose et al, 2001; Wang et al, 2009; Perez-Andres et al, 2011), normal B1 cells do not express CD3 (Griffin et al, 2011b). The addition of CD3-negative gating also reduces concerns that positive staining for any marker results from high autofluorescence and/or non-specific binding and is thus artifactual.…”

Section: Resultsmentioning

confidence: 99%

Human B1 Cell Frequency: Isolation and Analysis of Human B1 Cells

Griffin

Rothstein

2012

Front. Immun.

View full text Add to dashboard Cite

Controversy over the frequency of human B1 cells in normal individuals has arisen as different labs have begun to employ non-uniform techniques to study this population. The phenotypic profile and relative paucity of circulating human B1 cells place constraints on methodology to identify and isolate this population. Multiple steps must be optimized to insure accurate enumeration and optimal purification. In the course of working with human B1 cells we have developed a successful strategy that provides consistent analysis of B1 cells for frequency determination and efficient isolation of B1 cells for functional studies. Here we discuss issues attendant to identifying human B1 cells and outline a carefully optimized approach that leads to uniform and reproducible data.

show abstract

CD20-positive T cell leukemia/lymphoma: case report and review of the literature

Cited by 36 publications

References 9 publications

Level of PAX5 in differential diagnosis of non-Hodgkin′s lymphoma

Level of PAX5 in differential diagnosis of non-Hodgkin′s lymphoma

Relapsed angioimmunoblastic T-cell lymphoma with acquired expression of CD20: a case report and review of the literature

Human B1 Cell Frequency: Isolation and Analysis of Human B1 Cells

Contact Info

Product

Resources

About