CD19-SELECTED B LYMPHOCYTES SYNTHESIZE, SECRETE AND MIGRATE IN THE PRESENCE OF IL-8. TNF-α AND Γip-10 ARE ALSO B LYMPHOCYTE MIGRATORY FACTORS

Nielsen, Lene Svendstrup; Frydenberg, Jane; Lind, Martin; Deleuran, Mette; Stengaard‐Pedersen, Kristian

doi:10.1006/cyto.1997.0226

Cited by 17 publications

(11 citation statements)

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“…This suggests that IL-8 is interacting with an alternate chemokine receptor, or that the known receptors are present at the protein level, but not actually transcribed in ex vivo B cells, or are expressed below our detection sensitivity. Our findings are consistent with previous reports implicating IL-8 in B lymphocyte chemoattraction (46,59) and serve to extend these reports to a model that incorporates an endothelial barrier. Of interest, blocking IL-8 has been reported to have no effect on the migration of T cells across HBECs (36), suggesting that IL-8 secretion by resting HBECs may preferentially facilitate B lymphocyte migration, perhaps representing a mechanism that accounts for our observation that B cells migrated more efficiently than T cells across this barrier.…”

Section: Discussionsupporting

confidence: 92%

Determinants of Human B Cell Migration Across Brain Endothelial Cells

Alter

Duddy

Hebert

et al. 2003

The Journal of Immunology

174

123

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Circulating B cells enter the CNS as part of normal immune surveillance and in pathologic states, including the common and disabling illness multiple sclerosis. However, little is known about the molecular mechanisms that mediate human B cell interaction with the specialized brain endothelial cells comprising the blood-brain barrier (BBB). We studied the molecular mechanisms that regulate the migration of normal human B cells purified ex vivo, across human adult brain-derived endothelial cells (HBECs). We found that B cells migrated across HBECs more efficiently than T cells from the same individuals. B cell migration was significantly inhibited by blocking Abs to the adhesion molecules ICAM-1 and VLA-4, but not VCAM-1, similar to the results previously reported for T cells. Blockade of the chemokines monocyte chemoattractant protein-1 and IL-8, but not RANTES or IFN-γ-inducible protein-10, significantly inhibited B cell migration, and these results were correlated with the chemokine receptor expression of B cells measured by flow cytometry and by RNase protection assay. Tissue inhibitor of metalloproteinase-1, a natural inhibitor of matrix metalloproteinases, significantly decreased B cell migration across the HBECs. A comprehensive RT-PCR comparative analysis of all known matrix metalloproteinases and tissue inhibitors of metalloproteinases in human B and T cells revealed distinct profiles of expression of these molecules in the different cell subsets. Our results provide insights into the molecular mechanisms that underlie human B cell migration across the BBB. Furthermore, they identify potential common, and unique, therapeutic targets for limiting CNS B cell infiltration and predict how therapies currently developed to target T cell migration, such as anti-VLA-4 Abs, may impact on B cell trafficking.

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Section: Discussionsupporting

confidence: 92%

Determinants of Human B Cell Migration Across Brain Endothelial Cells

Alter

Duddy

Hebert

et al. 2003

The Journal of Immunology

174

123

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“…Furthermore, a majority of EBV-negative B-cell lines express IL-8R mRNA. We also observed CD19 enriched B-lymphocytes to express and secrete IL-8 as reported by Nielsen et al (24). However, we could not detect IL-8R transcripts suggesting no autocrine role for IL-8 in normal B-cells (data not shown).…”

Section: Discussionsupporting

confidence: 88%

Interleukin-8 Expression in AIDS-Associated Lymphoma B-Cell Lines

Sharma

Zhang

2001

Biochemical and Biophysical Research Communications

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“…These results are in accordance with the previous studies, which demonstrate that activated human B cells express and secrete CXCL8 [21-23]. We also demonstrate that the expression of CXCL1 chemokines, MIP-2 and KC, decline with age in murine splenocytes and B cells, particularly evident in MZ and FO B cell subsets (Fig.…”

Section: Resultssupporting

confidence: 93%

Untitled

Dixit

Coelho

et al. 2004

BMC Immunol

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BackgroundThe CXCL1 chemokines, macrophage inflammatory protein-2 (MIP-2) and cytokine-induced neutrophil chemoattractant (KC), have been shown to play a role in a number of pathophysiological disease states including endotoxin-induced inflammation and bacterial meningitis. While the expression of these chemokines has been identified in a variety of cell types in the mouse, little is known about their expression with murine B-lymphocytes.ResultsHere, we demonstrate that highly purified murine splenic B cells are capable of expressing both MIP-2 and KC protein and mRNA upon activation with lipopolysaccharide (LPS) but not in response to anti-μ and anti-CD40 in combination with interleukin-4 (IL-4) stimulation. Moreover, these chemokines are expressed at higher levels in B cells derived from young (4 m) compared to old (24–29 m) mice. Upon fractionation into distinct B-cell subsets, we found that the expression of MIP-2 and KC by aged follicular (FO) B cells is significantly decreased when compared to the same cells from younger mice, while only MIP-2 production was found to be diminished in aged marginal zone (MZ) B cells. Interestingly, MIP-2 and KC production by newly formed (NF) B cells did not significantly differ with age. Moreover, the potential relevance of these findings is supported by the poor ability of LPS-activated aged B cells to specifically mediate CXCL1-dependent leukocyte recruitment when compared to younger B cells.ConclusionOverall, the decreased expression of CXCL1 chemokines by aged B cells in response to LPS may have potential implications on the secondary recruitment of leukocytes to sites of microbial infections and inflammation possibly contributing to the increased susceptibility of older subjects to pathogen challenge.

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CD19-SELECTED B LYMPHOCYTES SYNTHESIZE, SECRETE AND MIGRATE IN THE PRESENCE OF IL-8. TNF-α AND Γip-10 ARE ALSO B LYMPHOCYTE MIGRATORY FACTORS

Cited by 17 publications

References 0 publications

Determinants of Human B Cell Migration Across Brain Endothelial Cells

Determinants of Human B Cell Migration Across Brain Endothelial Cells

Interleukin-8 Expression in AIDS-Associated Lymphoma B-Cell Lines

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