CD19 and POU2AF1 are Potential Immune-Related Biomarkers Involved in the Emphysema of COPD: On Multiple Microarray Analysis

Abstract

Purpose Emphysema is the main cause of the progression of chronic obstructive pulmonary disease (COPD). This study aimed to identify the key genes involved in COPD-related emphysema. Patients and Methods GSE76925 was downloaded from Gene Expression Omnibus database. Protein–protein interaction networks of differentially expressed genes (DEGs) between control and COPD groups were constructed to identify hub genes using Cytoscape. Diagnostic performance of hub genes was evaluated using receiver operating characteristic analysis. Correlation analysis was performed to identify the key genes by analyzing the relationship between the hub genes and lung function and computed tomography (CT) indexes of emphysema. COPD patients were then divided into two groups based on the median expression of key genes and DEGs between these two groups were identified. Enrichment analysis of DEGs and correlation analysis between key genes and the infiltration of the immune cells were also analyzed. Finally, the role of key genes was evaluated in a lung tissues dataset (GSE47460) and a blood dataset (GSE76705). Additionally, the expression of key genes was validated by quantitative real-time polymerase chain reaction and immunohistochemistry. Results CD19 and POU2AF1 had diagnostic efficacy for COPD and were significantly correlated with lung function and CT indexes of emphysema. Enrichment and immune analyses revealed that CD19 and POU2AF1 were correlated with the B cells in COPD. These results were consistent in GSE47460. The expression of CD19 and POU2AF1 in blood was the opposite of that in lung tissues, and CD19 and POU2AF1 were both significantly upregulated in COPD lung tissues at both the mRNA and protein levels. Conclusion CD19 and POU2AF1 may serve as key regulators of emphysema and contribute to the progression of COPD by regulating the B-cell immunology. Targeting B cells may be a promising strategy for treating COPD.

Single‐cell profiling of bronchoalveolar cells reveals a Th17 signature in neutrophilic severe equine asthma

“…S12/Table S9 provides the results of the DGE analysis for the major cell types, including B cells. The B cells of asthmatic horses upregulated POU2AF1 , whose elevated expression has been associated with interstitial pulmonary fibrosis [61] and chronic obstructive pulmonary disease [62] in humans. Its expression negatively correlates with lung function [62].…”

“…The B cells of asthmatic horses upregulated POU2AF1 , whose elevated expression has been associated with interstitial pulmonary fibrosis [61] and chronic obstructive pulmonary disease [62] in humans. Its expression negatively correlates with lung function [62]. Additionally, YBX3 was downregulated in the mast cells of asthmatic horses.…”

A novel iTreg‐related signature for prognostic prediction in lung adenocarcinoma

“…We therefore expect that the iTreg-related genes in the signature have prognostic have diagnostic efficacy for pulmonary disease and was significantly correlated with lung function. 47 Some studies have also explored the function and mechanisms of POU2AF1 in diseases. In multiple myeloma cells, POU2AF1, as a target of amplification at 11q23, promotes the growth of multiple myeloma cells by directly regulating the expression of B cell maturation factor TNFRSF17.…”

“…found dysregulation of POU2AF1 in abdominal aortic aneurysm and suggested it as a diagnostic biomarker for the condition 46 . POU2AF1 was found to have diagnostic efficacy for pulmonary disease and was significantly correlated with lung function 47 . Some studies have also explored the function and mechanisms of POU2AF1 in diseases.…”