CD177 Expression on Neutrophils

Meyerson, Howard; Osei, Ebeneezer; Schweitzer, Karen; Blidaru, Georgetta; Edinger, Alison; Balog, Anna

doi:10.1309/ajcpdfbebqzw1oi7

Cited by 22 publications

(18 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Our study population was made up of individuals of European, Asian and Australian self-reported ethnicity ( S2A Fig ). Consistent with previous reports [ 33 , 35 ], we identified three neutrophil populations according to CD177 expression: negative (neg), intermediate (int) and high (hi). The majority of individuals were bi-modal for CD177 (CD177 hi and CD177 neg ) ( Fig 1A ), while some individuals harbor a substantial proportion (>20%) of neutrophils expressing CD177 at intermediate levels ( Fig 1B ).…”

Section: Resultssupporting

confidence: 91%

Heterogeneity of Human Neutrophil CD177 Expression Results from CD177P1 Pseudogene Conversion

Liang

Ohnesorg

et al. 2016

PLoS Genet

View full text Add to dashboard Cite

Most humans harbor both CD177neg and CD177pos neutrophils but 1–10% of people are CD177null, placing them at risk for formation of anti-neutrophil antibodies that can cause transfusion-related acute lung injury and neonatal alloimmune neutropenia. By deep sequencing the CD177 locus, we catalogued CD177 single nucleotide variants and identified a novel stop codon in CD177null individuals arising from a single base substitution in exon 7. This is not a mutation in CD177 itself, rather the CD177null phenotype arises when exon 7 of CD177 is supplied entirely by the CD177 pseudogene (CD177P1), which appears to have resulted from allelic gene conversion. In CD177 expressing individuals the CD177 locus contains both CD177P1 and CD177 sequences. The proportion of CD177hi neutrophils in the blood is a heritable trait. Abundance of CD177hi neutrophils correlates with homozygosity for CD177 reference allele, while heterozygosity for ectopic CD177P1 gene conversion correlates with increased CD177neg neutrophils, in which both CD177P1 partially incorporated allele and paired intact CD177 allele are transcribed. Human neutrophil heterogeneity for CD177 expression arises by ectopic allelic conversion. Resolution of the genetic basis of CD177null phenotype identifies a method for screening for individuals at risk of CD177 isoimmunisation.

show abstract

Section: Resultssupporting

confidence: 91%

Heterogeneity of Human Neutrophil CD177 Expression Results from CD177P1 Pseudogene Conversion

Liang

Ohnesorg

et al. 2016

PLoS Genet

View full text Add to dashboard Cite

show abstract

“…CD177 protein expression measured by FACS became evident on stages differentiated to metamyelocytes and further. These data corroborate the data of Stroncek et al, who investigated CD177 expression on fetal bone marrow samples [35] and also those of Meyerson et al, who showed that the most mature neutrophils, expressing CD16 and CD11b also had the highest CD177 expression [22]. Mora-Jensen et al used the same method for isolating neutrophil stages from the bone marrow and investigated the expression of proteins representing the different granules [25].…”

Section: Discussionsupporting

confidence: 84%

“…CD177 has been described to be a counter receptor for platelet endothelial cell adhesion molecule (CD31) on endothelial cells [19], but not for platelets [20]. Recently it was reported that there is no correlation between decreased apoptosis rate of neutrophils in AAV and the proportion of CD177 + cells [21], and others showed that low expression of neutrophil CD177 was highly associated with clonal myeloid disorders [22]. Because of the lack of knowledge on the biological function for CD177, we performed a gene microarray-based study to investigate differences between CD177 + and CD177 − neutrophils, in order to investigate whether there is a pathophysiologic background of an increased CD177 + /mPR3 high neutrophil subset for the pathogenesis of AAV.…”

Section: Introductionmentioning

confidence: 99%

Differential Expression of Granulopoiesis Related Genes in Neutrophil Subsets Distinguished by Membrane Expression of CD177

Mora-Jensen

Theilgaard‐Mönch

et al. 2014

PLoS ONE

View full text Add to dashboard Cite

ObjectiveDifferential gene expression in CD177+ and CD177− neutrophils was investigated, in order to detect possible differences in neutrophil function which could be related to the pathogenesis of ANCA-associated Vasculitides (AAV).MethodsNeutrophils were isolated from healthy controls (HC) with high, negative or bimodal CD177 expression, and sorted into CD177+ and CD177− subpopulations. Total RNA was screened for expression of 24,000 probes with Illumina Ref-8 Beadchips. Genes showing differential expression between CD177+ and CD177− subsets in microarray analysis were re-assessed using quantitative-PCR. CD177 expression on neutrophil precursors in bone marrow was analyzed using quantitative PCR and flowcytometry.ResultsThe proportion of CD177+ cells increased during neutrophil maturation in bone marrow. Fold change analysis of gene expression profile of sorted CD177+ and CD177− neutrophils resulted in 14 genes with fold change (fc) >3 difference in expression. Interestingly, 10 of these genes have been reported to change significantly in expression during neutrophil maturation, and most of these genes were granule protein (GP) coding genes. mRNA expression levels measured by RT-PCR of a number of these GP, and of PR3 and MPO were higher in the CD177− neutrophil subset in HC, however, particular granule protein amounts were comparable between CD177+ and CD177− neutrophil subsets. AAV patients had higher amounts of CD177+ neutrophils, but contrary to neutrophils from HC expression of GP-genes was increased, possibly due to activation.ConclusionThe neutrophil population can be distinguished by membrane expression of CD177 into subsets that are different in expression of GP mRNA but not in GP protein production. GP gene expression is also elevated in AAV patients, which is not explained by skewed distribution of CD177+ and CD177− subsets but may be associated with neutrophil activation during on-going inflammation.

show abstract

“…Another interesting phenomenon that was not focus of our study is the occurrence of two CD177 pos peaks. Our observations are in agreement with the literature in that ∼5% of individuals show this CD177 pattern (Moritz et al, 2010;Meyerson et al, 2013;Wu et al, 2016). The molecular basis of this phenomenon is unclear and needs to be elucidated in future studies.…”

Section: Discussionsupporting

confidence: 92%

Gene silencing and a novel monoallelic expression pattern in distinct CD177 neutrophil subsets

Eulenberg-Gustavus

Bähring

Maass

et al. 2017

Journal of Experimental Medicine

View full text Add to dashboard Cite

show abstract

CD177 Expression on Neutrophils

Abstract: Skewed low neutrophil CD177(%) is highly associated with clonal myeloid disorders, particularly myelodysplasia, and may be useful for detecting clonal myeloid disorders.

Cited by 22 publications

References 41 publications

Heterogeneity of Human Neutrophil CD177 Expression Results from CD177P1 Pseudogene Conversion

Heterogeneity of Human Neutrophil CD177 Expression Results from CD177P1 Pseudogene Conversion

Differential Expression of Granulopoiesis Related Genes in Neutrophil Subsets Distinguished by Membrane Expression of CD177

Gene silencing and a novel monoallelic expression pattern in distinct CD177 neutrophil subsets

Contact Info

Product

Resources

About