CD166/ALCAM Expression Is Characteristic of Tumorigenicity and Invasive and Migratory Activities of Pancreatic Cancer Cells

Fujiwara, Kenji; Ohuchida, Kenoki; Sada, Masafumi; Horioka, Kohei; Ulrich, Charles D.; Shindo, Koji; Ohtsuka, Takao; Takahata, Shunichi; Mizumoto, Kazuhiro; Oda, Yoshinao; Tanaka, Masao

doi:10.1371/journal.pone.0107247

Cited by 48 publications

(43 citation statements)

References 30 publications

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“…Likewise, they demonstrated that ALCAM was highly expressed on the surface of prostate stem/ progenitor and cancer initiating cells (Jiao et al, 2012). In melanoma, pancreatic-and prostate cancers, up-regulation of ALCAM is associated with invasion and metastasis (Jannie et al, 2012;Fujiwara et al, 2014;Hansen et al, 2014). Our findings support these previous studies -ALCAM up-regulated both mRNA and protein levels when the CAA cell line was induced to invasive character.…”

Section: Discussionsupporting

confidence: 89%

“…The result of immunofluorescence found that ALCAM stained on the cytoplasmic membrane of CCA cell line correlated with increasing intensity in the cell treated with TNF-α. The location and increasing the expression of ALCAM in CCA cell line may suggest its roles in cell migration and invasion as found in other cancers (breast and pancreatic cancer) (Davies and Jiang, 2010;Fujiwara et al, 2014). In melanoma, silencing ALCAM expression in high-ALCAM expressed cell line a showed reduction of cell motility and invasiveness (Jannie et al, 2012).…”

Section: Discussionmentioning

confidence: 91%

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ALCAM is a Novel Cytoplasmic Membrane Protein in TNF-α Stimulated Invasive Cholangiocarcinoma Cells

Adisakwattana

Suwandittakul²,

Petmitr³

et al. 2015

Asian Pacific Journal of Cancer Prevention

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Background: Cholangiocarcinoma (CCA), or bile duct cancer, is incurable with a high mortality rate due to a lack of effective early diagnosis and treatment. Identifying cytoplasmic membrane proteins of invasive CCA that facilitate cancer progression would contribute toward the development of novel tumor markers and effective chemotherapy. Materials and Methods: An invasive CCA cell line (KKU-100) was stimulated using TNF-α and then biotinylated and purified for mass spectrometry analysis. Novel proteins expressed were selected and their mRNAs expression levels were determined by real-time RT-PCR. In addition, the expression of ALCAM was selected for further observation by Western blot analysis, immunofluorescent imaging, and antibody neutralization assay. Results: After comparing the proteomics profile of TNF-α induced invasive with non-treated control cells, over-expression of seven novel proteins was observed in the cytoplasmic membrane of TNF-α stimulated CCA cells. Among these, ALCAM is a novel candidate which showed significant higher mRNA-and protein levels. Immunofluorescent assay also supported that ALCAM was expressed on the cell membrane of the cancer, with increasing intensity associated with TNF-α. Conclusions: This study indicated that ALCAM may be a novel protein candidate expressed on cytoplasmic membranes of invasive CCA cells that could be used as a biomarker for development of diagnosis, prognosis, and drug or antibody-based targeted therapies in the future.

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Section: Discussionsupporting

confidence: 89%

Section: Discussionmentioning

confidence: 91%

ALCAM is a Novel Cytoplasmic Membrane Protein in TNF-α Stimulated Invasive Cholangiocarcinoma Cells

Adisakwattana

Suwandittakul²,

Petmitr³

et al. 2015

Asian Pacific Journal of Cancer Prevention

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“…The expression of ALCAM has been reported to be upregulated in a range of cancers, including melanoma, colon cancer, and gastric cancer where it has a role in promoting cell motility and migration, leading to more invasive phenotypes (11,22,36). In lung and pancreatic cancers, inhibition of ALCAM has been shown to have enhanced invasive and migratory properties of the cells (17,33). In the context of pregnancy, developmental biology studies have shown that ALCAM expression generally occurs in cells undergoing proliferation and is required for implantation of blastocysts (17).…”

Section: Discussionmentioning

confidence: 99%

“…In lung and pancreatic cancers, inhibition of ALCAM has been shown to have enhanced invasive and migratory properties of the cells (17,33). In the context of pregnancy, developmental biology studies have shown that ALCAM expression generally occurs in cells undergoing proliferation and is required for implantation of blastocysts (17). ALCAM has previously been shown to be downregulated in placental tissue (24) and deciduas basalis (12) from pregnancies complicated with preeclampsia.…”

Section: Discussionmentioning

confidence: 99%

DNA methylation profiles in preeclampsia and healthy control placentas

Yeung

Chiu

Pidsley

et al. 2016

American Journal of Physiology-Heart and Circulatory Physiology

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Preeclampsia is a hypertensive disorder of pregnancy that affects 3-5% of all pregnancies. There is evidence to suggest that epigenetic mechanisms, such as DNA methylation, play a role in placental development and function. This study compared DNA methylation profiles of placentas from preeclampsia-affected pregnancies with placentas from healthy pregnancies to identify gene-specific changes in DNA methylation that may contribute to the development of preeclampsia. The methylation status of eight placental biopsies taken from preeclampsia-affected and 16 healthy pregnancies was analyzed using the Illumina Infinium Methylation 450 BeadChip array. Bisulfite pyrosequencing was used to confirm regions found to be differentially methylated between preeclampsia and healthy placentas. A total of 303 differentially methylated regions, 214 hypermethylated and 89 hypomethylated, between preeclampsia cases and controls were identified, after adjusting for gestational age (adjusted P < 0.05). Functional annotation found cell adhesion, wingless type MMTV Integration Site family member 2 (Wnt) signaling pathway, and regulation of transcription were significantly enriched in these gene regions. Hypermethylation of WNT2, sperm equatorial segment protein (SPESP1), NADPH oxidase 5 (NOX5), and activated leukocyte cell adhesion molecule (ALCAM) in preeclampsia placentas was confirmed with pyrosequencing. This study found differences in methylation in gene regions involved in cell signaling (WNT2), fertilization and implantation (SPESP1), reactive oxygen species signaling (NOX5), and cell adhesion (ALCAM). These results build on recently published studies that have reported significant differences in DNA methylation in preeclampsia placentas.

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“…When the cells become cancerous, CD166, which is located on the outer surface of colorectal cancer cells, is noticeably overexpressed at the surface [14].…”

Section: Introductionmentioning

confidence: 99%

In Silico Analysis, Cloning and Expression of Recombinant CD166 in E. coli BL21 (DE3) as a Marker for Detection and Treatment of Colorectal Cancer

Marmari¹,

Mahmoodzadeh²,

Dana³

et al. 2017

J Med Microb Diagn

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CD166/ALCAM Expression Is Characteristic of Tumorigenicity and Invasive and Migratory Activities of Pancreatic Cancer Cells

Cited by 48 publications

References 30 publications

ALCAM is a Novel Cytoplasmic Membrane Protein in TNF-α Stimulated Invasive Cholangiocarcinoma Cells

ALCAM is a Novel Cytoplasmic Membrane Protein in TNF-α Stimulated Invasive Cholangiocarcinoma Cells

DNA methylation profiles in preeclampsia and healthy control placentas

In Silico Analysis, Cloning and Expression of Recombinant CD166 in E. coli BL21 (DE3) as a Marker for Detection and Treatment of Colorectal Cancer

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