CD164 and other recently discovered activation markers as promising tools for allergy diagnosis: what’s new?

Chirumbolo, Salvatore

doi:10.1007/s10238-011-0132-y

Cited by 8 publications

(9 citation statements)

References 19 publications

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“…Purification of basophils represents a time-consuming and expensive approach; attempts to ameliorate and enrich the endowment of those molecules able to assess basophil function during an allergic response have been widely made. Some activation markers, such as CD164, CD107a or also CD13 and CD69, have been proposed, but their use in BAT as a widely used application in routine diagnosis is still far from being achieved [112,113]. Why this gap in BAT technical improvement?…”

Section: The Use Of Basophil Flow Cytometry In Allergy Diagnosis: Stamentioning

confidence: 99%

Basophil Activation Test in Allergy: Time for an Update?

Chirumbolo

2012

Int Arch Allergy Immunol

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A wide range of reported evidence in the literature has shown that the quantification of basophil activation by flow cytometry (basophil activation test, BAT) has proven to be a useful tool for the assessment of immediate-type responses to allergens mediated by IgE or other mechanisms in allergic patients. The usefulness of BAT in anaphylactic adverse reactions, late-onset allergy and immunotherapy follow-up has also been demonstrated. To date, most BAT studies reported in the literature involved the capture of basophils only with a fluorochrome-labeled anti-IgE antibody and application of CD63 upregulation in order to evaluate the basophil response to allergens. Many issues need to be addressed, such as optimizing the analytical performance of the test, checking preanalytical conditions, the selection of the flow cytometry best gating protocol, the introduction of new algorithms and parameters, the search for new activation markers and the introduction of anti-IgE controls. BAT is certainly a useful technique, also for isolated cases of hypersensitivity to various other compounds and drugs, and an update of its application is certainly an interesting topic to expand the debate on allergy diagnosis.

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Section: The Use Of Basophil Flow Cytometry In Allergy Diagnosis: Stamentioning

confidence: 99%

Basophil Activation Test in Allergy: Time for an Update?

Chirumbolo

2012

Int Arch Allergy Immunol

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“…Previous studies have revealed that CD164 was involved in tumor progression via the regulation of cell proliferation and apoptosis in several cancers 13, 14, 15, 23, 32. In addition, CD164 was implicated in regulating the migration and invasion of lung cancer and medulloblastoma cells 17, 18.…”

Section: Discussionmentioning

confidence: 98%

CD164 promotes tumor progression and predicts the poor prognosis of bladder cancer

Zhang

Li³

et al. 2018

Cancer Medicine

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CD164 was found to play a role in many malignant diseases. But the roles of CD164 in human bladder cancer have not yet been studied. The object of our study was to investigate the functions of CD164 in urothelial bladder carcinoma. The immunohistochemistry (IHC) was performed to evaluate the associations between the expression level of CD164 and clinical‐pathological features of patients, and IHC was used to analyze the relationship between CD164 and CXCR4 in tumor tissues. Real‐time qPCR and Western blot were used to measure the expression of relevant genes. The roles of CD164 in tumor cells and tissues were investigated by in vitro and in vivo experiments. The results of immunohistochemistry found that CD164 was associated with clinical and pathological features of patients. High level of CD164 was related to the distant metastasis and vascular invasion of bladder cancer patients. In vitro, by silencing of CD164, the proliferation, migration, and invasion of tumor cells were inhibited significantly by regulating related proteins such as Ki67, proliferating cell nuclear antigen, matrix metalloproteinases‐2, and matrix metalloproteinases‐9. In vivo, knocking‐down of CD164 could reduce the growth and metastasis of tumors in mice. In addition, a co‐expression was found between CD164 and CXCR4 in tumor tissues. In conclusion, our study demonstrated that CD164 was associated with the poor clinical outcomes of BC patients. Silencing of CD164 could inhibit the progression of tumors in vivo and in vitro, which may become an effective target in the treatment of bladder cancer.

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“…The Authors stressed on the commonly recognized role exerted by CD63 and CD203c markers in BAT, while existing further suggestions [2,3] but they never addressed the critical issue of basophil electronic capture in flow cytometry (FC), i.e. cell phenotyping protocols [4,5].…”

Section: To the Editormentioning

confidence: 99%