CD151 promotes proliferation and migration of PC3 cells via the formation of CD151-integrin α3/α6 complex

Abstract

Over-expression of CD151 was found to be associated with metastasis and poor prognosis of prostatic carcinoma. This study was designed to examine the mechanism by which CD151 promotes the proliferation and migration of prostatic cancer cells. The pAAV-CD151, pAAV-GFP and pAAV-CD151-AAA mutant plasmids were constructed and used to transiently transfect PC3 cells (a prostatic carcinoma 3 cell line) by the mediation of Fugene HD. Then, the cells were assigned to control group, pAAV-GFP group, pAAV-CD151 group, and pAAV-CD151-AAA group respectively. Cell proliferation was evaluated by using the 3-[4,5-dimet-hylthiazol-2-yl]-2,5, diphenyltetrazolium bromide (MTT) method. Cell migration assay was performed by using Boyden chambers. The formation of CD151-integrin α3/α6 complex was determined by the method of co-immunoprecipitation. The protein expression levels of CD151 and extracellular signal-regulated kinase (ERK) were measured by Western blotting. The results showed that transfection of pAAV-CD151 or pAAV-CD151-AAA mutant increased the expression of CD151 protein in PC3 cells. Co-immunoprecipitation showed that more CD151-integrin α3/α6 complex was formed in the pAAV-CD151 group than in the control group, the pAAV-GFP group and the pAAV-CD151-AAA mutant group. Furthermore, the proliferative and migrating capacity of PC3 cells was substantially increased in the pAAV-CD151 group but inhibited in the pAAV-CD151-AAA mutant group. CD151 transfection increased the expression of phospho-ERK. Taken together, it was concluded that CD151 promotes the proliferation and migration of PC3 cells through the formation of CD151-integrin complex and the activation of phosphorylated ERK.

Laminin-511

“…This would be consistent with the recent demonstration that LM-511 promotes proliferation and partially blocks EMT in human β cells 80 . An interesting possibility is that these distinct LM-511 and LM-332-dependent adhesive responses during EMT and MET may be differentially regulated by CD151, an important modulator of LM-binding integrins 66 , 81 , 82 . CD151 overexpression was shown recently to be an independent prognostic marker of poor overall survival in invasive breast cancer, particularly in quintuple negative breast cancer, a subtype of TN breast cancer 83 .…”

Tetraspanin CD151 mediates communication between prostate cancer cells and osteoblasts

“…It was reported that promigratory function of CD151 in PC3 cells was based on regulation of ERK activity (Yang et al, 2012). We additionally showed that presence of CD151 facilitates phosphorylation of FAK, Src and HSP27 (considered as directly involved in regulation of cell migration) in response to osteoblasts-conditioned medium.…”

Genetic Ablation of the Tetraspanin CD151 Reduces Spontaneous Metastatic Spread of Prostate Cancer in the TRAMP Model

“…Molecular studies involving CD151 with cancer progression and metastasis to date have focused on in vitro and grafting models. These studies have shown that knockdown/-out or mutation of CD151 decreases cell motility (16)(17)(18)(19)(20)(21)(22) and/or decreases experimental metastasis (23)(24)(25)(26).…”