CD147 overexpression promotes tumorigenicity in Chinese hamster ovary cells

Abstract

CD147 overexpresses in many epithelium-originated tumors and plays an important role in tumor migration and invasion. Most studies aim at the role of CD147 in tumor progression using tumor cell models. However, the influence of abnormal overexpression of CD147 on neoplastic transformation of normal cells is unknown. Here, the role of CD147 in malignant phenotype transformation in CHO cells was investigated. Three CHO cell lines that stably overexpressed CD147 (CHO-CD147), EGFP-CD147 (CHO-EGFP-CD147), and EGFP (CHO-EGFP) were generated by transfection of plasmids containing human CD147, EGFP-human CD147, and EGFP genes into CHO cells. Cell migration and invasion were detected by wound healing and transwell matrix penetration assay. Trypan blue exclusion, MTT, cell cycle analysis, and BrdU cell proliferation assay were used to detect cell viability and cell proliferation. Annexin V-FITC analysis was performed to detect apoptosis. We found that CD147 overexpression promoted the migration and invasion of CHO cells. CD147 accelerated the G1 to S phase transition and enhanced the CHO cell proliferation. Overexpression of CD147 inhibited both early- and late-stages of apoptosis of CHO-CD147 cells, which is caused by serum deprivation. CHO-EGFP-CD147 cells showed an increased anchorage-independent growth compared with CHO-EGFP cells as detected by soft-agar colony formation assay. The tumors formed by CHO-CD147 cells in nude mice were larger and coupled with higher expression of proliferating cell nuclear antigen and Ki-67 than that of CHO cells. In conclusion, human CD147 overexpression induces malignant phenotype in CHO cells.

CD147 promotes glucose metabolism, invasion and metastasis via PI3K/AKT pathway in oral squamous cell carcinomas

“…Therefore, metastasis is a rational therapeutic target for oral cancer ( 31 , 33 ). CD147, originally isolated from the plasma membranes of malignant tumor cells ( 34 , 35 ), is an important modulator of the anchorage-independent growth and invasiveness of tumor cells ( 36 , 37 ). However, the mechanistic role of CD147 in oral cancer, especially squamous cell carcinoma, is largely ambiguous.…”

The CD147‐HYALURONAN Axis in Cancer

“…However, as noted above, CD147 may not be a direct inducer of these proteases but may act indirectly via stimulation of assembly of MCTs and other metabolic transporters (Gallagher et al, ; Marchiq et al, ; Kendrick et al, ) or by influencing the activities of other MMP‐regulatory factors. Most important, nevertheless, are studies showing that experimentally increased CD147 promotes tumor growth, local invasion, and metastases, as well as MMP production, in several cancer cell types in vivo and that inhibition of CD147 expression has the opposite effects (Zucker et al, ; Xu et al, ; Huang et al, ; Walter et al, ; Yong et al, ; Hu et al, ; Voss et al, ). Moreover, a striking relationship between CD147 expression and human tumor metastasis has been demonstrated (Klein et al, ; Reimers et al, ).…”