CD146 (Mel-CAM), an adhesion marker of endothelial cells, is a novel marker of lymphocyte subset activation in normal peripheral blood

Elshal, Mohamed F.; Khan, Sameena S.; Takahashi, Yoshiyuki; Solomon, Michael A.; McCoy, J. Philip

doi:10.1182/blood-2005-06-2307

Cited by 104 publications

(95 citation statements)

References 5 publications

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“…In contrast to humans [19], we failed to detect any expression of CD146 on mouse B and T lymphocytes (Fig. 3A).…”

Section: A Fraction Of Neutrophils Express Cd146contrasting

confidence: 58%

“…Phenotypic and genomic analyses have revealed that CD146 1 T cells are a population of effector memory T cells [18]. Along the same lines, CD146 expression on T and B cells can be induced in vitro upon activation with PHA or CD40L1IL-4, respectively [19]. These data suggest that in vivo CD146 expression on lymphocytes could represent a marker of chronically activated lymphocyte subpopulations, but its expression in murine lymphocytes remains poorly investigated.…”

mentioning

confidence: 99%

“…In human thymus, CD146 is detected in endothelial cells, smooth muscle cells of blood vessels, epithelial cells, thymocytes and macrophages and could play a role in the maintenance of a thymic architecture [17]. Conversely, human peripheral blood leukocytes were found to be mainly CD146-negative cells since only 2% of B and T lymphocytes express CD146 at steady-state conditions [18,19]. CD146 expression was also reported on T lymphocytes infiltrating delayed-type hypersensitivity lesions of the skin, on synovial fluid T cells of rheumatoid arthritis patients and on some T-cell lines [20].…”

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confidence: 99%

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Mouse CD146/MCAM is a marker of natural killer cell maturation

et al. 2008

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CD146/melanoma cell adhesion molecule is an adhesion molecule expressed by endothelial cells and by a small fraction of activated T and B lymphocytes in humans. In order to analyze the pattern of CD146 expression in mouse leukocytes at steady-state conditions, we generated a set of novel rat anti-mouse CD146 monoclonal antibodies. CD146 expression was undetectable on monocytes, dendritic cells, T cells or B cells, but was expressed on about 30% of neutrophils and 60% of NK cells. Within murine lymphocytes, CD146 was defined as a novel NK-specific surface molecule. An increased percentage of CD146 1 cells was found in the most mature CD27 À CD11b 1 NK cell subpopulation, which also displays higher expression of Ly49C/I, Ly49D and KLRG1 and lower expression of NKG2A/C/E molecules. CD146 1 NK cells were found to be less cytotoxic and produce less IFN-c than CD146 À NK cells upon stimulation with target cells or activating antibodies. These findings define CD146 as a marker of mouse NK cell maturation that may be used as an alternative to the combined use of CD27 and CD11b staining to detect final stages of NK cell maturation.Key words: Adhesion . Antibodies . CD146/MCAM . Maturation . NK cells Introduction CD146, also known as melanoma cell adhesion molecule (MCAM or Mel-CAM), MUC18, S-Endo1, A32 antigen, is an integral membrane glycoprotein of 113 kDa that belongs to the Ig superfamily with a characteristic V-V-C2-C2-C2 domain structure [1]. Two others members of this family have been identified in humans: basal-cell adhesion molecule and activated leukocytecell adhesion molecule, also called CD166 [2,3]. Basal-cell adhesion molecule is a splice variant of the Lutheran blood group glycoprotein that is reported to bind laminin [3]. Activated leukocyte-cell adhesion molecule is a costimulatory molecule mediating homophilic interaction and heterophilic interaction with CD6 and is involved in activation and trafficking of leukocytes into the central nervous system [4]. CD146 was originally defined as a marker of tumor progression and metastasis formation in human melanoma [5]. Overexpression of CD146 in CD146-negative melanoma cells renders these cells highly metastatic after injection in immunodeficient mice [6]. Moreover, injection of a humanized anti-CD146 mAb in immunodeficient mice bearing human melanomas significantly reduces the tumor size and the number of metastases [7]. The adhesive functions of CD146 were demonstrated using a solid-phase adhesion test in which both CD146-positive and CD146-negative melanoma cells bound to eucaryotic recombinant CD146 protein, suggesting that CD146 can mediate both homophilic and heterophilic interaction with an as yet unidentified ligand [8,9]. Using S-Endo1 mAb, we previously detected cell surface expression of CD146 on human vascular endothelial cells, whatever vessel caliber or anatomical region [10]. On cultured endothelial cells, CD146 is mainly located at the intercellular junctions and is involved in inter-endothelial cell adhesion and paracellular permeability [11,1...

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“…In contrast to humans [19], we failed to detect any expression of CD146 on mouse B and T lymphocytes (Fig. 3A).…”

Section: A Fraction Of Neutrophils Express Cd146contrasting

confidence: 58%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Mouse CD146/MCAM is a marker of natural killer cell maturation

et al. 2008

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“…The BD Biosciences P1H12 clone and BioCytex S-Endo 1 clone do not stain CMOIC and lymphocytes in any dilution (Panels Ai-iii, Ci,iii). Plotting both CMOIC and lymphocytes using CD45 vs. CD146 bivariate histograms reveals the presence of a minor CD146þ lymphocyte subset as expected (11), whilst CMOIC are CD146 negative with these mAb (Panels Bi-iii, Diiii). In contrast, the Chemicon P1H12 reacts positively with CMOIC at 0.2 mg/test, but this is due to high background fluorescence, as the total lymphocyte population shows a similar staining pattern (Panels Ei-iii).…”

Section: Fluorescence In Situ Hybridizationmentioning

confidence: 99%

“…Expression of CD146 is known to be limited to endothelial cells (1,16,17) and a subset of activated T-cells (11,18). Two mAb derived from the P1H12 clone (one from BD Biosciences and one from Chemicon [Temecula, CA]), and a third mAb derived from the S-Endo 1 clone (BioCytex [Marseille, France]) were titrated on HUVEC, CMOIC, and lymphocytes (CD45 bright , SSC low ).…”

Section: Cmoic Do Not Express Cd146mentioning

confidence: 99%

Cells meeting our immunophenotypic criteria of endothelial cells are large platelets

Strijbos

Kraan

Bakker

et al. 2007

Cytometry Part B Clinical

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Endothelial progenitor cells in cardiovascular diseases

Qiu

Zhang

et al. 2018

Aging Medicine

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Cardiovascular diseases (CVDs) are the leading cause of death in both developed and developing countries. Endothelial progenitor cells (EPCs) are derived from hematopoietic stem cells with powerful function of angiogenesis. There are many studies on the relation between coronary heart disease and circulating EPCs. In this review, we discuss biological characteristics of endothelial progenitor cells, some influencing factors of the number and function of EPCs, and the role of EPCs in the treatment of cardiovascular disease. At last, we bring some perspectives on the future of endothelial progenitor cell therapy.

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CD146 (Mel-CAM), an adhesion marker of endothelial cells, is a novel marker of lymphocyte subset activation in normal peripheral blood

Cited by 104 publications

References 5 publications

Mouse CD146/MCAM is a marker of natural killer cell maturation

Mouse CD146/MCAM is a marker of natural killer cell maturation

Cells meeting our immunophenotypic criteria of endothelial cells are large platelets

Endothelial progenitor cells in cardiovascular diseases

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