2010
DOI: 10.1016/j.radonc.2009.10.010
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CD133 expression is not selective for tumor-initiating or radioresistant cell populations in the CRC cell line HCT-116

Abstract: CRC cell lines could be classified into three groups: (i) CD133-, (ii) CD133+ and (iii) those with two distinct CD133+ and CD133- subpopulations. Isolated CD133+/- HCT-116 subpopulations were studied relative to the original fraction. No difference was found in 2-D growth, spheroid formation or radioresponse in vitro. Also, tumor formation and growth rate did not differ for the sorted subpopulations. However, a subset of xenografts originated from CD133- HCT-116 showed a striking enrichment in the CD133+ fract… Show more

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Cited by 30 publications
(30 citation statements)
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“…Routine verification of cell line purity and clonality was detailed earlier for HCT-116 cells. 21 Authenticity of cell lines was confirmed by microsatellite analyses at the Institute of Legal Medicine (Dresden University of Technology) using the commercial multiplex PCR kits Mentype NonaplexQS Twin (Biotype, Germany) and PowerPlex 16 (Promega Corporation, USA). Amplicons were detected by capillary electrophoresis in denaturing polymer POP4 in an ABI 310 sequencer (Perkin-Elmer, USA) according to the manufacturer's instructions.…”
Section: Materials and Methods Cell Lines And Culture Routinesmentioning
confidence: 99%
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“…Routine verification of cell line purity and clonality was detailed earlier for HCT-116 cells. 21 Authenticity of cell lines was confirmed by microsatellite analyses at the Institute of Legal Medicine (Dresden University of Technology) using the commercial multiplex PCR kits Mentype NonaplexQS Twin (Biotype, Germany) and PowerPlex 16 (Promega Corporation, USA). Amplicons were detected by capillary electrophoresis in denaturing polymer POP4 in an ABI 310 sequencer (Perkin-Elmer, USA) according to the manufacturer's instructions.…”
Section: Materials and Methods Cell Lines And Culture Routinesmentioning
confidence: 99%
“…An advanced anti-CD133 staining protocol was applied as described earlier. 21 Briefly, cells were incubated for 30 min in the dark at 41C with a PE (phycoerythrin)-conjugated anti-CD133 antibody (CD133/1-PE or isotype, Miltenyi Biotec) diluted 1:100 in PBS containing 0.5% FCS and 2 mM EDTA followed by signal enhancement via a two-step FASER series (Fluorescence Amplification by Sequential Employment of Reagents) according to the manufacturer's instructions (Miltenyi Biotec). Cell suspensions originated from xenograft tumors were also co-stained with an anti-human CD326-FITC (fluorescein isothiocyanate) antibody (dilution 1:25; Miltenyi Biotec) to discriminate human epithelial and mouse stromal cells.…”
Section: Materials and Methods Cell Lines And Culture Routinesmentioning
confidence: 99%
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