CD133+ bone marrow stem cells (BMSC) control platelet activation – Role of ectoNTPDase-1 (CD39)

Duhme, Constanze; Lehwald, Nadja; Kehrel, Beate E.; Bauchrowitz, Ellen; Ngepi, Arlette; Schmelzle, Moritz; Kolokotronis, Theodoros; Benhidjeb, Tahar; Krüger, Martin; Jurk, Kerstin; Knoefel, Wolfram Trudo; Robson, Simon C.; Esch, Jan Schulte Am

doi:10.1016/j.bcmd.2019.04.012

Cited by 3 publications

(4 citation statements)

References 39 publications

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“…These results are in agreement with the previously reported studies performed on platelets using CD133 + bone marrow, lipoaspirate and cord blood stem cells. 44,45 As previously reported, pMSCs display immunomodulatory properties making them useful therapeutic agents for various diseases. 31 They show surface expression of important modulators and secrete an array of soluble factors that exert immune responses on T cells, B cells, monocytes and macrophages.…”

Section: Discussionmentioning

confidence: 84%

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Human Placental Mesenchymal Stem/Stromal cells (pMSCs) inhibit agonist‐induced platelet functions reducing atherosclerosis and thrombosis phenotypes

Subayyil

Basmaeil

Alenzi

et al. 2021

J Cellular Molecular Medi

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Cardiovascular diseases (CVDs) are the number one cause of global death and disability. According to WHO, an estimated 17.9 million lives are consumed each year by the CVDs. 1 CVDs represent a group of disorders involving heart and blood vessels. It is mainly associated with atherosclerosis and an increased risk of blood clots. 1Atherosclerosis is typically characterized by plaque formation affecting different vascular beds. [2][3][4] Platelet activation and hyperreactivity have been thought to contribute towards pathogenesis of acute atherothrombotic disorders, such as myocardial infarction (MI) and stroke. 5 Although the classical role of platelets is maintenance of

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Section: Discussionmentioning

confidence: 84%

“…These results are in agreement with the previously reported studies performed on platelets using CD133 + bone marrow, lipoaspirate and cord blood stem cells. 44 , 45 …”

Section: Discussionmentioning

confidence: 99%

Human Placental Mesenchymal Stem/Stromal cells (pMSCs) inhibit agonist‐induced platelet functions reducing atherosclerosis and thrombosis phenotypes

Subayyil

Basmaeil

Alenzi

et al. 2021

J Cellular Molecular Medi

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“…Other platelet stimulators were not tested to that respect. Our group previously demonstrated a regulatory effect of CD133 + BMSC for ADP-dependent platelets aggregation in co-incubation of these two cell-types characterized as NTPDase1 (CD39) dependent [ 8 ]. This is in line with a report on eosinophils to directly inhibit platelet aggregation among others due to ADP-stimulation [ 73 ].…”

Section: Discussionmentioning

confidence: 99%

“…Others showed an important role of hematopoietic CD133 + stem cells interacting with platelets resulting in increased SDF-1 expression [ 7 ]. Interestingly, we recently demonstrated CD133 + BMSC when co-incubated with platelets to control their thrombogenic responses in a CD39-dependent manner [ 8 ]. Furthermore, a major role for platelets was described in the mediation of hepatic injury [ 9 , 10 , 11 ].…”

Section: Introductionmentioning

confidence: 99%

Platelets Boost Recruitment of CD133+ Bone Marrow Stem Cells to Endothelium and the Rodent Liver—The Role of P-Selectin/PSGL-1 Interactions

Lehwald

Duhme

Pinchuk

et al. 2020

IJMS

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We previously demonstrated that clinical administration of mobilized CD133+ bone marrow stem cells (BMSC) accelerates hepatic regeneration. Here, we investigated the potential of platelets to modulate CD133+BMSC homing to hepatic endothelial cells and sequestration to warm ischemic livers. Modulatory effects of platelets on the adhesion of CD133+BMSC to human and mouse liver-sinusoidal- and micro- endothelial cells (EC) respectively were evaluated in in vitro co-culture systems. CD133+BMSC adhesion to all types of EC were increased in the presence of platelets under shear stress. This platelet effect was mostly diminished by antagonization of P-selectin and its ligand P-Selectin-Glyco-Ligand-1 (PSGL-1). Inhibition of PECAM-1 as well as SDF-1 receptor CXCR4 had no such effect. In a model of the isolated reperfused rat liver subsequent to warm ischemia, the co-infusion of platelets augmented CD133+BMSC homing to the injured liver with heightened transmigration towards the extra sinusoidal space when compared to perfusion conditions without platelets. Extravascular co-localization of CD133+BMSC with hepatocytes was confirmed by confocal microscopy. We demonstrated an enhancing effect of platelets on CD133+BMSC homing to and transmigrating along hepatic EC putatively depending on PSGL-1 and P-selectin. Our insights suggest a new mechanism of platelets to augment stem cell dependent hepatic repair.

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Bone Marrow Mesenchymal Stem Cells (BMSC) Homing with Chemotaxis Transplantation of Stromal Cell-Derived Factor 1a Promotes the Corneal Damage Repair

Zhang

Dai

Lin

et al. 2023

j biomater tissue eng

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This study analyzed the effect of bone marrow mesenchymal stem cells (BMSC) homing with chemotaxis transplantation of SDF-1a on the repair of corneal damage. The SDF-1a with varied concentration was added. They were divided into A group, B group, C group, D group and control group followed by analysis of corneal cell survival by MTT, apoptosis by flow cytometry, and Trkb level by immunohistochemical staining. There was an increasing tendency on the quantity of chemotactic cells (P <0.05) with a highest quantity in C group. The recruitment of BMSC could be prompted by SDF-1a and the chemotactic effect was the best when SDF-1a concentration was 100 ng/ml. The survival rate and Trkb protein level in experimental groups was higher than that in control group with highest survival rate and Trkb level in C group. In conclusion, corneal injury repair is prompted by BMSC homing with chemotaxis transplantation of SDF-1a, indicating that it might be used as a novel approach to promote corneal injury repair.

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CD133+ bone marrow stem cells (BMSC) control platelet activation – Role of ectoNTPDase-1 (CD39)

Cited by 3 publications

References 39 publications

Human Placental Mesenchymal Stem/Stromal cells (pMSCs) inhibit agonist‐induced platelet functions reducing atherosclerosis and thrombosis phenotypes

Human Placental Mesenchymal Stem/Stromal cells (pMSCs) inhibit agonist‐induced platelet functions reducing atherosclerosis and thrombosis phenotypes

Platelets Boost Recruitment of CD133+ Bone Marrow Stem Cells to Endothelium and the Rodent Liver—The Role of P-Selectin/PSGL-1 Interactions

Bone Marrow Mesenchymal Stem Cells (BMSC) Homing with Chemotaxis Transplantation of Stromal Cell-Derived Factor 1a Promotes the Corneal Damage Repair

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