CD11c/CD18 Dominates Adhesion of Human Monocytes, Macrophages and Dendritic Cells over CD11b/CD18

Sándor, Noémi; Lukácsi, Szilvia; Ungai-Salánki, Rita; Orgován, Norbert; Szabó, Bálint; Horváth, Róbert; Erdei, Anna; Bajtay, Zsuzsa

doi:10.1371/journal.pone.0163120

Cited by 77 publications

(95 citation statements)

References 54 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Though both CR3 and CR4 are able to bind fibrinogen and iC3b, we found that either of them dominates the other in various functions, such as antigen uptake, digestion, in addition to adhesion and spreading, which we had shown earlier (Sándor, 2016).…”

Section: Discussionsupporting

confidence: 66%

The role of CR3 (CD11b/CD18) and CR4 (CD11c/CD18) in complement-mediated phagocytosis and podosome formation by human phagocytes

et al. 2017

Self Cite

View full text Add to dashboard Cite

CR3 and CR4 belong to the family of β 2 -integrins and play an important role in phagocytosis, cellular adherence and migration. CR3 and CR4 are generally expected to mediate similar functions due to their structural homology, overlapping ligand specificity and parallel expression on human phagocytes. Although the different signalling pathways of these receptors suggest distinct functions, possible differences are just being revealed.Previously we proved that CR3 plays a key role in the uptake of iC3b-opsonized particles by human dendritic cells. Now, besides measuring the overall phagocytic capacity of cells including the assessment of surface bound as well as internalized particles, we extended our investigations and studied the digestion of the iC3b opsonized antigen by various human phagocytes. The participation of CR3 and CR4 was compared in the process of binding, internalization and digestion of iC3b opsonized Staphylococcus aureus by monocytes, monocyte derived macrophages (MDMs), monocyte derived dendritic cells (MDDCs), and neutrophils. Comparing the activity of the two β 2 -integrin type complement receptors we found that CR3 plays a dominant role in the phagocytosis of iC3b opsonized S. aureus by all of these cell types. Studying another important integrin-mediated function we demonstrated earlier thatCR4 is dominant in the adhesion of monocytes, MDMs and MDDCs to fibrinogen. Here we studied the participation of CR3 and CR4 in podosome formation by human phagocytes, since these structures are known to play an essential role in cell migration. Our confocal microscopy analysis revealed that both CD11b and CD11c concentrate in the podosome adhesion ring. In summary our data highlight differences in the function of human CR3 and CR4 in the process of uptake and digestion of complement opsonized antigen, while in the process of podosome formation, connected to cellular motility, both receptors equally take part. Keywordsphagocytosis, podosomes, human phagocytes, β 2 integrins, CR3 (CD11b/CD18), CR4 (CD11c/CD18) Highlights -CR3 is the dominant receptor mediating the phagocytosis of iC3b opsonized S. aureus -surface bound and digested bacteria should be distinguished -MDDCs have a unique podosome pattern compared to monocytes, MDMs and neutrophils -both CR3 and CR4 are located in the adhesion ring of podosomes

show abstract

Section: Discussionsupporting

confidence: 66%

The role of CR3 (CD11b/CD18) and CR4 (CD11c/CD18) in complement-mediated phagocytosis and podosome formation by human phagocytes

et al. 2017

Self Cite

View full text Add to dashboard Cite

show abstract

“…The CD11b, CD11c and CD18 leukointegrins play a role in phagocytosis, cell‐to‐cell and cell‐to‐extracellular matrix . Our data indicate similarity with those in the literature, where reports have shown that L. chagasi infection induces decreased expression of CD11b/CD18 in macrophages .…”

Section: Discussionsupporting

confidence: 89%

Leishmania amazonensis induces modulation of costimulatory and surface marker molecules in human macrophages

Costa

Fornazim

Nowill

et al. 2018

Parasite Immunology

View full text Add to dashboard Cite

Manipulation of costimulatory and surface molecules that shape the extent of immune responses by Leishmania is suggested as one of the mechanisms of evading the host's defences. The experiments reported here were designed to evaluate the expressions of CD11b, CD11c, CD14, CD18, CD54, CD80, CD86, CD206, MHC class II and TLR-2 (Toll-like receptor 2) in human macrophages infected with L. amazonensis. Phenotypic evaluation revealed a negative modulation in CD11b, CD11c, CD14, CD18, CD54 and MHC class II molecules, depending on the level of infection. The results showed that as early as 1 hour after infection no reduction in marker expression occurs, whereas after 24 hours, downregulation of these molecules was observed in macrophages. No significant changes were observed in the expressions of CD80, CD86, CD206 and TLR2. Evidence of the differential modulation of markers expression and that after parasite uptake no reduction in surface marker expression occurs indicates that parasite internalization is not involved in the phenomena of down-modulation.

show abstract

“…Although CR3 and CR4 were thought to exert similar functions, recently we showed that they have different roles in various human myeloid cells. Namely, while CR3 dominates phagocytosis in the case of dendritic cells [3], CR4 is more important in mediating adherence by human monocytes macrophages and dendritic cells [2].…”

Section: Introductionmentioning

confidence: 97%

“…These receptors mediate several important cellular functions, including adhesion, trans-endothelial migration and interaction with extracellular matrix proteins [1]. By interaction with iC3b, the complement-derived natural ligand, CR3 and CR4 mediate the uptake of opsonized cells and particles by monocytes, macrophages, neutrophils and dendritic cells [2]. Although CR3 and CR4 were thought to exert similar functions, recently we showed that they have different roles in various human myeloid cells.…”

Section: Introductionmentioning

confidence: 99%

Functional studies of chronic lymphocytic leukemia B cells expressing β 2 -integrin type complement receptors CR3 and CR4

et al. 2017

Self Cite

View full text Add to dashboard Cite

The expression and role of CR3 (CD11b/CD18) and CR4 (CD11c/CD18) in B cellsare not yet explored in contrast to myeloid cells, where these β 2 -integrin type receptors are known to participate in various cellular functions, including phagocytosis, adherence and migration. Here we aimed to reveal the expression and role of CR3 and CR4 in human B cells. In B cells of healthy donors CR3 and CR4 are scarcely expressed. However, two patients with chronic lymphocytic leukemia (CLL) characterized by a peculiar immunephenotype containing both CD5-positive and CD5-negative B cell populations made possible to study these molecules in distinct B cell subsets. We found that CD11b and CD11c were expressed on both CD5-positive and CD5-negative B cells, albeit to different extents. Our data suggest that these receptors are involved in spreading, since this activity of CpGactivated B cells on fibrinogen could be partially blocked by monoclonal antibodies specific for CD11b or CD11c. CpG-stimulation lead to proliferation of both CD5-positive and CD5-negative B cells of the patients with a less pronounced effect on the CD5-positive cells. In contrast to normal B cells, CLL B cells of both patients reacted to CpG-stimulation with robust IL-10 production. The concomitant, suboptimal stimulus via the BCR and TLR9 exerted either a synergistic enhancing effect or resulted in inhibition of proliferation and IL-10 production of patients' B cells.Our data obtained studying B cells of leukemic patients point to the role of CR3 in the interaction of tumor cells with the microenvironment and suggest the involvement of IL-10 producing B cells in the pathologic process.

show abstract

CD11c/CD18 Dominates Adhesion of Human Monocytes, Macrophages and Dendritic Cells over CD11b/CD18

Cited by 77 publications

References 54 publications

The role of CR3 (CD11b/CD18) and CR4 (CD11c/CD18) in complement-mediated phagocytosis and podosome formation by human phagocytes

The role of CR3 (CD11b/CD18) and CR4 (CD11c/CD18) in complement-mediated phagocytosis and podosome formation by human phagocytes

Leishmania amazonensis induces modulation of costimulatory and surface marker molecules in human macrophages

Functional studies of chronic lymphocytic leukemia B cells expressing β 2 -integrin type complement receptors CR3 and CR4

Contact Info

Product

Resources

About