CD105(+) cells from Wharton's jelly show in vitro and in vivo myogenic differentiative potential

Abstract

Abstract. Embryo-derived tissues, such as umbilical cord (UC), can represent attractive sources of mesenchymal stem cells because their use is not related to any ethical issue. Abundant experimental evidence has already shown that Wharton's jelly contains cells able to differentiate in vitro into adipocytes, chondrocytes, osteocytes and neurons. Human UCs were obtained from term caesarean deliveries and processed within 24 h. Cells derived from the Wharton's jelly expressing mesenchymal markers, such as CD105, but not KDR and CD31 antigens, have been selected by positive and negative immunoseparation. These cells were characterized by an elongated shape and a good proliferation rate. Moreover, they were, at least in part, of fetal origin, as demonstrated by the expression of Sry mRNA. The expression of Myf5 and MyoD was detected after 7 and 11 days of in vitro myogenic induction, respectively. At two weeks from cell injection in the tibialis anterior muscle, previously damaged with bupivacaine, skeletal muscle appeared completely repaired and transplanted cells were present in the muscle for two weeks and differentiated into skeletal muscle cells, as demonstrated by the co-localization of HLA 1 and sarcomeric tropomyosine antigens. These observations provide the first demonstration that CD105(+)/CD31(-)/KDR(-) cells are able not only to differentiate in vivo towards the myogenic lineage, but also to contribute to the muscle regenerative process.

Development of human umbilical cord matrix stem cell-based gene therapy for experimental lung tumors

“…UCMS cells transplanted into rodent neurodegenerative models [4][5][6] have been extensively characterized 3 and they express many of the genes expressed by primitive stem cells such as embryonic stem cells (ESCs). 7 They can be differentiated into a variety of cell types including muscle, 8 cartilage, 9 bone, 9 and neural 1 cells and have been used for tissue-engineered artificial blood vessels and heart valves. 10 Previously, several types of stem cells have been shown to migrate selectively into tumors.…”

Human umbilical cord-derived mesenchymal stem cells differentiate into epidermal-like cells using a novel co-culture technique

“…Previous studies have reported the in vitro differentiation of hUCMSCs into mesoderm-type cells, such as osteoblasts, chondrocytes, adipocytes, cardiomyocytes, skeletal muscle cells, and endothelial cells Conconi et al 2006;Hoerstrup et al 2002;Sarugaser et al 2005;Schmidt et al 2006;Wang et al 2004;Wu et al 2007) as well as cells of nonmesoderm lineages, including neurocytes (Wang et al 2004;Fu et al 2004;Ma et al 2005;Mitchell et al 2003). hUC blood-derived MSCs enhanced wound healing in mice by differentiating into keratinocytes (Luo et al 2010).…”

Fast-Degradable Microbeads Encapsulating Human Umbilical Cord Stem Cells in Alginate for Muscle Tissue Engineering

“…The myogenic inductive medium consisted of high-glucose DMEM, 20% FBS, 1% penicillin-streptomycin-glutamine, and 10 mM 5-azacytidine (5-Aza). 14,29,30 The myogenic proliferative medium had high-glucose DMEM, 20% FBS, 1% penicillin-streptomycin-glutamine, 10% HS, and 1% CEE. Both 5-Aza and HS are myogenic supplements.…”

“…Both 5-Aza and HS are myogenic supplements. 14,29,30 Following the established methods, 14 hUCMSCs were cultured in the myogenic inductive medium for 2 days and then cultured in the myogenic proliferative medium. As detailed in previous studies, the myogenic proliferative medium was used after 2 days with HS to continue to induce myogenesis.…”

“…[10][11][12][13] Only a few studies investigated hUCMSCs for muscle engineering by culturing on dishes, or injecting into animals. [14][15][16] To date, there has been no report on using a three-dimensional scaffold to seed hUCMSCs for myogenic differentiation.…”