CD10 protein expression in tumor and stromal cells of malignant melanoma is associated with tumor progression

Abstract

CD10 antigen is a 100-kDa-cell surface zinc metalloendopeptidase expressed in a variety of normal and neoplastic lymphoid and nonlymphoid tissues including melanomas. It was recently shown that metastatic melanomas express more CD10 than primary tumors. We evaluated CD10 expression in tumor and stromal cells in 70 biopsies with primary and 28 with metastatic malignant melanomas. Ki-67, Bcl-2, and Bax were also examined to investigate whether CD10 expression is associated with tumor proliferation index or factors of apoptosis. Formalin-fixed/paraffin-embedded tissues were studied by immunohistochemistry. More advanced primary tumors had higher CD10 expression in the tumor cells (r ¼ 0.27, P ¼ 0.03 for Clark levels and r ¼ 0.29, P ¼ 0.02 for Breslow) and higher Ki-67 proliferation fraction (r ¼ 0.32, P ¼ 0.007 for Clark levels and r ¼ 0.32, P ¼ 0.001 for Breslow). Similarly, CD10 expression in the intratumoral stromal cells was also higher in primary tumors with higher Clark level (P ¼ 0.04, linear-by-linear association) and tumor thickness according to Breslow (r ¼ 0.33, P ¼ 0.01). The presence of CD10 þ peritumoral stromal cell cuffs was also positively associated with tumor thickness according to Breslow (r ¼ 0.27, P ¼ 0.05). Also, expression of CD10 and Ki-67 were significantly higher in metastatic than in primary tumors (P ¼ 0.01 and 0.02 respectively), but Bcl-2 expression was higher in primary melanomas (P ¼ 0.02). We conclude that CD10 expression in malignant melanoma is associated with tumor progression.

Follicular center helper T-cell (TFH) marker positive mycosis fungoides/Sezary syndrome

“…[2][3][4][5]49 Perifollicular dermal sheath fibroblasts and dermal fibroblasts may express CD10 in inflammatory and neoplastic skin conditions. [2][3][4][5][6][7][8] We noted staining of this sort in some of our specimens. The strong staining of CD10 in dermal fibroblasts associated with the lymphoma cells made it difficult to identify lymphoma cell specific staining as has been previously noted.…”

“…Similar reactivity has been noted in association with non-hematopoietic and lymphoid cutaneous neoplasms and inflammatory skin conditions. [6][7][8][9][10]42 One patient (patient 9) demonstrated only rare CD10( þ ) cells by immunohistochemistry despite having CD10( þ ) mycosis fungoides/Sezary syndrome cells in the peripheral blood. This patient had 5-15% abnormal CD10( þ )CD4( þ ) T cells detected in the blood in eight separate samples over 3 years.…”

“…1 CD10 expression has been described on a number of cells types and neoplasms that involve skin including the perifollicular fibrous sheath of the hair follicle, dermal fibroblasts, neutrophils, germinal center B cells, lymphomas of germinal center cell origin, melanoma, atypical fibroxanthoma and a variety of mesenchymal tumors. [2][3][4][5][6][7][8][9][10][11][12] Additionally, CD10 is expressed on a subset of CD4( þ ) T cells residing in the lymph node follicle germinal center termed follicular helper T cells. 13 These T cells are also known to express PD-1, CXCL-13, CD57, CXCR5, SAP, and ICOS with their differentiation controlled by Bcl-6.…”

“…26 Detection of CD10 on tumor cells in skin biopsies by immunohistochemistry can be difficult due to expression of CD10 on tumor-associated dermal fibroblasts obscuring the cellular origin of the staining. 6,7,42 As an alternative to immunohistochemistry, CD10 on tumor cells can be detected by flow cytometry. Previously, we have had success in evaluating T cells isolated from skin biopsies using flow cytometry.…”

Alteration of extracellular superoxide dismutase expression is associated with an aggressive phenotype of oral squamous-cell carcinoma

“…as negative controls, the slides were incubated with pBS instead of primary antibodies. to quantitate the state of Ec-SOd protein expression in these of components, we used previously described histo (h)-score systems (21,22). in brief, the mean percentage of the epithelial cells that showed a persistent Ec-SOd signal was respectively determined in at least five distinct fields in each section at a magnification of x400.…”