CD‐sens: a biological measure of immunological changes stimulated by ASIT

Nopp, Anna; Cardell, Lars-Olaf; Johansson, S. G. O.; Öman, H.

doi:10.1111/j.1398-9995.2008.01900.x

Cited by 66 publications

(84 citation statements)

References 15 publications

(29 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…However, the dosage had to be increased to 4-fold that recommended for patients with their IgE body pool. Even though the long-term effect of Xolair seems to be very beneficial, followed by a withdrawal without side effects or rebound phenomena [10], the introduction of ASIT, monitored by CD-sens [11], under Xolair protection [12], could be considered.…”

Section: Discussionmentioning

confidence: 99%

Xolair Is Effective in Allergics with a Low Serum IgE Level

Ankerst¹,

Nopp²,

Johansson³

et al. 2009

Int Arch Allergy Immunol

Self Cite

View full text Add to dashboard Cite

Background: Two atopic patients suffering from severe allergy difficult to handle by conventional medication were given Xolair despite an IgE level <30 kU/l. Methods: Increasing dosages were given and monitored by clinical evaluation and CD-sens to clinically relevant allergens. The patients’ IgE antibody fractions were 11–14%. Results: Xolair dosages extrapolated from a recommended dose for IgE of 30–75 kU/l were adapted to the patients’ IgE body pool but had very little effect. The double dose resulted in some clinical improvement and a decrease in CD-sens. However, not until the dose was doubled again did the patients become symptom free, although 1 patient needed some additional drugs but no oral steroids. CD-sens turned negative to 5 of the 7 tested allergens. Conclusions: Xolair is most useful also in atopics with an IgE level <30 kU/l. The dose must be adjusted to the size of the IgE antibody fraction adding all non-cross-reacting, clinically relevant specificities.

show abstract

Section: Discussionmentioning

confidence: 99%

Xolair Is Effective in Allergics with a Low Serum IgE Level

Ankerst¹,

Nopp²,

Johansson³

et al. 2009

Int Arch Allergy Immunol

Self Cite

View full text Add to dashboard Cite

show abstract

“…The suppression of basophil activation after discontinuation of AIT has been demonstrated 12-24 months following discontinuation of treatment [Nopp et al 2009;Lalek et al 2010;Gokmen et al 2012;Shamji et al 2015;Zidarn et al 2015].…”

Section: Cellular Changesmentioning

confidence: 99%

Applications and mechanisms of immunotherapy in allergic rhinitis and asthma

Kappen

Durham

Veen

et al. 2016

Ther Adv Respir Dis

View full text Add to dashboard Cite

Clinical and immunologic tolerance are hallmarks of successful allergen immunotherapy (AIT). Clinical benefits such as reduced symptoms, pharmacotherapy intake and improvement of quality of life persist following cessation of treatment. Successful AIT is associated with suppression of allergic inflammatory cells such as mast cells, eosinophils and basophils in target organs. Furthermore, AIT down-regulates type 2 innate lymphoid cells and allergen-specific type 2 T-helper (Th2) cells. The immunologic tolerant state following AIT is associated with the induction of distinct phenotypes of regulatory T-cells (T-regs) including interleukin (IL)-10-, IL-35-and transforming growth factor (TGF)-β-producing T-regs and FoxP3 + T-regs. B-cell responses, including the induction of IL-10 + regulatory B-cells (B-regs) and the production of IgG4-associated blocking antibodies are also induced following successful AIT. These events are associated with the suppression of antigen-specific Th2 responses and delayed immune deviation in favour of Th1 type responses. Insight into the mechanisms of AIT has allowed identification of novel biomarkers with potential to predict the clinical response to AIT and also novel therapeutic strategies for more effective and safer AIT.

show abstract

“…Furthermore, during pollen or Hymenoptera venom immunotherapy, basophil allergen sensitivity is significantly decreased [26,27,34,35,36]. In pollen immunotherapy, the obvious reason for these decreases is the induction of allergen-specific IgG antibodies with inhibitory activity against IgE-facilitated binding, and these antibodies showed sustained disease and cell-modifying effects that persisted for years after the discontinuation of immunotherapy [35,36,37].…”

Section: Discussionmentioning

confidence: 99%

Distinct Contributory Factors Determine Basophil-Allergen Sensitivity in Grass Pollen Rhinitis and in Anaphylactic Wasp Venom Allergy

Korošec

Šilar²,

Kopač³

et al. 2016

Int Arch Allergy Immunol

View full text Add to dashboard Cite

Background: We sought to determine whether basophil-allergen sensitivity could be transferred to donor basophils by passive IgE sensitisation in allergic rhinitis and anaphylactic Hymenoptera venom hypersensitivity. Methods: We studied 15 wasp venom-, 19 grass pollen- and 2 house dust mite-allergic patients, 2 healthy donors, and 8 wasp venom-allergic donors. In all subjects, we first evaluated the initial basophil response to wasp venom, grass pollen, or house dust mite allergen. Donor basophils were then stripped, sensitised with the different patients' serum IgE, and challenged with the corresponding allergen. The CD63 response of donor basophils was then compared with initial basophil responses. Results: In wasp venom-allergic subjects, the IgE transfer did not reflect the initial basophil-allergen sensitivity, because the venom IgE of subjects with high or low basophil sensitivity induced comparable responsiveness in healthy donor basophils. Furthermore, vice versa, when we sensitised the donor basophils of wasp venom-allergic individuals with different wasp venom or house dust mite IgE, we demonstrated that their response was predictable by their initial basophil allergen sensitivity. In the rhinitis allergy model, the IgE transfer correlated with the patients' initial basophil responsiveness because the grass pollen IgE of the subjects with high basophil allergen sensitivity induced significantly higher responsiveness of donor basophils than the IgE of subjects with initially low basophil allergen sensitivity. Conclusions: Our results suggest that basophil allergen sensitivity evaluated by flow-cytometric CD63 analysis depends on two distinct contribution factors. In anaphylactic Hymenoptera allergy, the major factor was intrinsic cellular sensitivity, whereas in pollen allergy, the major factor was allergen-specific IgE on the cell surface.

show abstract

CD‐sens: a biological measure of immunological changes stimulated by ASIT

Abstract: CD-sens and ABA, in contrast to basophil reactivity, seem to be promising tools to monitor protective immune responses initiated by ASIT.

Cited by 66 publications

References 15 publications

Xolair Is Effective in Allergics with a Low Serum IgE Level

Xolair Is Effective in Allergics with a Low Serum IgE Level

Applications and mechanisms of immunotherapy in allergic rhinitis and asthma

Distinct Contributory Factors Determine Basophil-Allergen Sensitivity in Grass Pollen Rhinitis and in Anaphylactic Wasp Venom Allergy

Contact Info

Product

Resources

About