Cd adsorption onto Pseudomonas putida in the presence and absence of extracellular polymeric substances

Ueshima, Masato; Ginn, Brian R.; Haack, Elizabeth A.; Szymanowski, Jennifer E. S.; Fein, Jeremy B.

doi:10.1016/j.gca.2008.09.014

Cited by 157 publications

(81 citation statements)

References 35 publications

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“…46 It is possible that if we separated the EPS, it could sorb metal ions; however, in the presence of S. putrefaciens cells at the higher density of 2 g dw L À1 , the processes of Mn(II) phosphate and Mn(II) carbonate bioprecipitation were dominating at the conditions described in this work.…”

Section: -45mentioning

confidence: 78%

Formation of manganese phosphate and manganese carbonate during long-term sorption of Mn²⁺by viable Shewanella putrefaciens: effects of contact time and temperature

Chubar

Avramut

Visser

2015

Environ. Sci.: Processes Impacts

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The influence of temperature (5, 10, 22 and 30 C) on the long-term (30 days) sorption of Mn 2+ by viable Shewanella putrefaciens was studied by FTIR and EXAFS. The additional Mn-removal capacity of these bacteria was found to result from the surface precipitation of Mn-containing inorganic phases. The chemical composition of the Mn-containing precipitates is temperature and contact-time dependent.Mn(II) phosphate and Mn(II) carbonate were the two major precipitates formed in 1000 mL batches at 10, Environmental impactThe data shown in this work demonstrate that viable S. putrefaciens (and most likely some other microorganisms) has a great potential to stabilise (or reduce the mobility in the environment of) Mn 2+ (and most likely some other metal ions) through processes occurring at the interfaces of the cells for at least 30 days. The interfacial processes start from ion exchange/surface complexation and continue with biomineralisation. Composition of bioprecipitates is a function of temperature, metal loading and bacterial density. The results presented demonstrate that the role of microorganisms in formation of natural minerals might be even greater than thought preciously.

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Section: -45mentioning

confidence: 78%

Formation of manganese phosphate and manganese carbonate during long-term sorption of Mn²⁺by viable Shewanella putrefaciens: effects of contact time and temperature

Chubar

Avramut

Visser

2015

Environ. Sci.: Processes Impacts

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“…These techniques yield reliable information on biofilm structure (78,79), chemical composition (24,80), and heavy metal adsorption (24,28). Moreover, CLSM can be used to explore dynamic microbial population change (24,81) and mass transport in biofilm systems (78).…”

Section: Applicationsmentioning

confidence: 99%

“…Newport Green and Fluozin-1 are well-established metal fluorescent probes with relatively poor selectivity; Newport Green shows different dissociation constants and thus sensitivities to nickel, cadmium, and zinc, while Fluozin-1 shows different dissociation constants for cadmium and zinc since they have different binding sites. Several studies of hydrated biofilms applying dual labeling of bacterial cells and the respective metals have revealed that Ni, Zn, and Cd show reasonably heterogeneous distributions in biofilms (28)(29)(30). In all of these studies, bacterial cell walls bound and enriched metal ions due to cellular sorption.…”

mentioning

confidence: 99%

Mapping of Heavy Metal Ion Sorption to Cell-Extracellular Polymeric Substance-Mineral Aggregates by Using Metal-Selective Fluorescent Probes and Confocal Laser Scanning Microscopy

Hao

Kappler

et al. 2013

Appl Environ Microbiol

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b Biofilms, organic matter, iron/aluminum oxides, and clay minerals bind toxic heavy metal ions and control their fate and bioavailability in the environment. The spatial relationship of metal ions to biomacromolecules such as extracellular polymeric substances (EPS) in biofilms with microbial cells and biogenic minerals is complex and occurs at the micro-and submicrometer scale. Here, we review the application of highly selective and sensitive metal fluorescent probes for confocal laser scanning microscopy (CLSM) that were originally developed for use in life sciences and propose their suitability as a powerful tool for mapping heavy metals in environmental biofilms and cell-EPS-mineral aggregates (CEMAs). The benefit of using metal fluorescent dyes in combination with CLSM imaging over other techniques such as electron microscopy is that environmental samples can be analyzed in their natural hydrated state, avoiding artifacts such as aggregation from drying that is necessary for analytical electron microscopy. In this minireview, we present data for a group of sensitive fluorescent probes highly specific for Fe 3؉ , Cu 2؉ , Zn 2؉ , and Hg 2؉ , illustrating the potential of their application in environmental science. We evaluate their application in combination with other fluorescent probes that label constituents of CEMAs such as DNA or polysaccharides and provide selection guidelines for potential combinations of fluorescent probes. Correlation analysis of spatially resolved heavy metal distributions with EPS and biogenic minerals in their natural, hydrated state will further our understanding of the behavior of metals in environmental systems since it allows for identifying bonding sites in complex, heterogeneous systems. Biofilms are the dominant form of microbial life on Earth (1), and the organic material that is present in biofilms significantly impacts the cycling and sequestration of toxic heavy metals in the environment (2, 3). The underlying sorption and complexation mechanisms are difficult to evaluate (4), since biofilms are highly dynamic and complex structures that consist of diverse biomacromolecules (5) and the in situ heavy metal distributions are readily influenced by common invasive analysis approaches such as sequential extractions for the determination of different metal fractions. Here, we introduce a promising approach for studying the distribution and sorption of heavy metals in biofilms and cell-extracellular polymeric substance (EPS)-mineral aggregates (CEMAs) under close-to-natural conditions using confocal laser scanning microscopy (CLSM) in combination with highly selective metal ion-sensitive fluorescence probes. This approach is frequently used for metal detection in cell biology but was rarely applied in environmental and geomicrobiology research due to a former lack of highly selective fluorescence probes and due to the complexity of environmental biofilms and CEMAs.Biofilms are mainly composed of water, microorganisms, and an EPS matrix (6) that consists of mostly polysacchari...

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“…The FTIR spectra of both full EPS-and partial EPS-bacteria were normalized to the height of the peak at 1539 cm −1 for S. suis and 1545 cm −1 for E. coli (amide II), respectively. Following the same protocol, the supernatants of bacterial suspensions were also scanned as the background spectra (Ueshima et al, 2008).…”

Section: Atr-ftir Spectroscopymentioning

confidence: 99%

“…Potentiometric titration was performed by an automatic potentiometric titrator (Metrohm titrator 836, Metrohm, Switzerland) to analyze the acid-base properties of full EPS-and partial EPS-pathogen surfaces (Fein et al, 2005;Ueshima et al, 2008;Kenny and Fein, 2011). Bacterial suspensions with concentrations of 6.0 × 10 9 cells per mL in 0.01 M KCl were titrated using 0.09 M HCl and 0.09 M KOH solutions under a N 2 atmosphere at 25°C.…”

Section: Potentiometric Titrationmentioning

confidence: 99%

Contrasting effects of extracellular polymeric substances on the surface characteristics of bacterial pathogens and cell attachment to soil particles

et al. 2015

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Cd adsorption onto Pseudomonas putida in the presence and absence of extracellular polymeric substances

Cited by 157 publications

References 35 publications

Formation of manganese phosphate and manganese carbonate during long-term sorption of Mn²⁺by viable Shewanella putrefaciens: effects of contact time and temperature

Formation of manganese phosphate and manganese carbonate during long-term sorption of Mn²⁺by viable Shewanella putrefaciens: effects of contact time and temperature

Mapping of Heavy Metal Ion Sorption to Cell-Extracellular Polymeric Substance-Mineral Aggregates by Using Metal-Selective Fluorescent Probes and Confocal Laser Scanning Microscopy

Contrasting effects of extracellular polymeric substances on the surface characteristics of bacterial pathogens and cell attachment to soil particles

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Cd adsorption onto Pseudomonas putida in the presence and absence of extracellular polymeric substances

Cited by 157 publications

References 35 publications

Formation of manganese phosphate and manganese carbonate during long-term sorption of Mn2+by viable Shewanella putrefaciens: effects of contact time and temperature

Formation of manganese phosphate and manganese carbonate during long-term sorption of Mn2+by viable Shewanella putrefaciens: effects of contact time and temperature

Mapping of Heavy Metal Ion Sorption to Cell-Extracellular Polymeric Substance-Mineral Aggregates by Using Metal-Selective Fluorescent Probes and Confocal Laser Scanning Microscopy

Contrasting effects of extracellular polymeric substances on the surface characteristics of bacterial pathogens and cell attachment to soil particles

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Formation of manganese phosphate and manganese carbonate during long-term sorption of Mn²⁺by viable Shewanella putrefaciens: effects of contact time and temperature

Formation of manganese phosphate and manganese carbonate during long-term sorption of Mn²⁺by viable Shewanella putrefaciens: effects of contact time and temperature