CCR5/CCR5 ligand-induced myeloid-derived suppressor cells are related to the progression of endometriosis

Guo, Peipei; Bi, Kaihuan; Lu, Zhimin; Wang, Kangxia; Xu, Yuping; Wu, Huan; Cao, Yunxia; Jiang, Huanhuan

doi:10.1016/j.rbmo.2019.05.014

Cited by 19 publications

(29 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Heparinized PB was obtained from all participants immediately before surgery. Plasma and peripheral blood mononuclear cells (PBMCs) were collected from samples following previous protocols 40 . The PBMC samples were adjusted to 10 7 cells before cryopreservation at −80°C.…”

Section: Methodsmentioning

confidence: 99%

“…Details of mAbs for MAIT cells, MDSCs and Tregs are shown in Table 2. Cell staining procedure followed previous studies 40 . Cell acquisition was performed by FACSVerse and FACSuite software with compensation control (BD Biosciences) implemented according to manufacturer's instructions.…”

Section: Methodsmentioning

confidence: 99%

“…Plasma and peripheral blood mononuclear cells (PBMCs) were collected from samples following previous protocols. 40 The PBMC samples were adjusted to 10 7 cells before cryopreservation at −80 C. Table 2. Cell staining procedure followed previous studies.…”

Section: Patient Cohort and Sample Collectionmentioning

confidence: 99%

“…MDSCs have always been considered a key part in the immunopathogenesis and progression of tumors and other diseases. 11,[38][39][40] It has previously been demonstrated that circulating MDSCs from cervical cancer patients was elevated compared to those in controls, and that these MDSCs extensively suppressed proliferation of T cells. 11 As MAIT is one of the unconventional T cells, their correlation with MDSCs in the progression of cervical cancer has been poorly investigated.…”

Section: Introductionmentioning

confidence: 99%

“…Plasma cytokine analysis via ELISA ELISA kits (Multisciences Biotech, Hangzhou, China) were used to analyze cytokines in plasma according to manufacturer's protocol and previous studies 40. …”

mentioning

confidence: 99%

See 4 more Smart Citations

The combined action of monocytic myeloid‐derived suppressor cells and mucosal‐associated invariant T cells promotes the progression of cervical cancer

Zhu

Marley

et al. 2020

Intl Journal of Cancer

Self Cite

View full text Add to dashboard Cite

One of the most common promoters of the initiation and growth of the tumor is an immune disturbance. Numerous immune cells and inflammatory factors play a role in the tumor‐immune microenvironment. However, few studies have investigated the correlation between these immunological events and clinical consequences in cervical cancer. We measured the levels of numerous inflammatory mediators and frequencies of regulatory T cells (Tregs), myeloid‐derived suppressor cells (MDSCs) and mucosal‐associated invariant T (MAIT) cells in peripheral blood (PB) of cervical cancer patients. Cervical cancer patients showed elevated production of interleukin (IL)‐18 and plasma C‐C chemokine ligand (CCL) 3/5. Meanwhile, an accumulation of C‐C chemokine receptor 5 (CCR5) monocytic (Mo)‐MDSCs and Tregs was observed. The cervical cancer group displayed increased frequencies of CD8+, CD4+ and highly activated CD38+CD8+MAIT cells, and reduction of double‐negative (DN) and PD1(CD279+) DN MAIT cells. Importantly, it was demonstrated that MAIT cells were positively related to Mo‐MDSCs. Furthermore, an elevated concentration of PD1(CD279+) DN MAIT cells was significantly related to increased progression‐free survival of patients with cervical cancer. In conclusion, our study suggests that the combined action of Mo‐MDSCs and MAIT cells might be associated with the progression of cervical cancer, and the frequency of DN MAIT cells in the peripheral blood mononuclear cells was associated with the survival benefit of patients.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Patient Cohort and Sample Collectionmentioning

confidence: 99%