CCND1-associated ceRNA network reveal the critical pathway of TPRG1-AS1-hsa-miR-363-3p-MYO1B as a prognostic marker for head and neck squamous cell carcinoma

Abstract

Head and neck squamous cell carcinoma (HNSC) is one of the leading causes of cancer death globally, yet there are few useful biomarkers for early identification and prognostic prediction. Previous studies have confirmed that CCND1 amplification is closely associated with head and neck oncogenesis, and the present study explored the ceRNA network associated with CCND1. Gene expression profiling of the Head and Neck Squamous Cell Carcinoma (HNSC) project of The Cancer Genome Atlas (TCGA) program identified the TPRG1-AS1-hsa-miR-363-3P-MYO1B gene regulatory axis associated with CCND1. Further analysis of the database showed that MYOB was regulated by methylation in head and neck tumors, and functional enrichment analysis showed that MYO1B was involved in "actin filament organization" and "cadherin binding ". Immune infiltration analysis suggested that MYO1B may influence tumorigenesis and prognosis by regulating the immune microenvironment of HNSC. MYO1B enhanced tumor spread through the EMT approach, according to epithelial mesenchymal transition (EMT) characterisation. We analyzed both herbal and GSCALite databases and found that CCND1 and MYO1B have the potential as predictive biomarkers for the treatment of HNSC patients. RT-qPCR validated bioinformatic predictions of gene expression in vitro cell lines. In conclusion, we found a CCND1-related ceRNA network and identified the novel TPRG1-AS1-hsa-miR-363-3p-MYO1B pathway as a possible HNSC diagnostic biomarker and therapeutic target.

lncRNA TPRG1-AS1 Screened the Onset of Acute Coronary Syndromes and Predicted Severity and the Occurrence of MACE During Patients’ Hospitalization

“…7 TPRG1-AS1 was also identified as a prognostic biomarker of head and neck squamous cell carcinoma and promoted the development of human cancers. [8][9][10] Therefore, TPRG1-AS1 was speculated to be involved in the pathogen and development of ACS, which lacks confirmed data in current reports. Serum TPRG1-AS1 level can be analyzed by PCR assay, which could provide a rapid quantification.…”

Upregulated lncRNA LINC01128 in colorectal cancer accelerates cell growth and predicts malignant prognosis through sponging miR-363-3p

“…In a recent report by Wang et al, miR-363-3p was proposed to be decreased in colorectal cancer and may mediate tumorigenesis by directly targeting IFITM1 (Wang et al 2023a ). In addition, miR-363-3p was confirmed to be downregulated in polycystic ovary syndrome, ovarian serous cystadenocarcinoma, and head and neck squamous cell carcinoma (Li et al 2023 ; Sang et al 2023 ; Zhang et al 2023 ). Furthermore, binding sites were predicted between miR-363-3p and LINC01128, and miR-363-3p was the downstream action target of LINC01128.…”

Competing Endogenous RNAs (ceRNAs) and Application of Their Regulatory Networks in Complex Traits and Diseases of Ruminants

“…For example, Zhang et al [88] used ceRNA networks to elucidate mechanisms in disease pathology, particularly cancer research. Li et al [89] investigated physiologic cellular and tissue function and adopted ceRNA networks to identify pathogenesis. In pharmaceutical studies, ceRNA networks have shed light on drug effects in cancer models [90,91].…”