CCL2 promoter hypomethylation is associated with gout risk in Chinese Han male population

Li, Bin; Chen, Xiaoying; Jiang, Yuting; Yang, Yong; Zhong, Jie; Zhou, Cong; Hu, Haochang; Duan, Shiwei

doi:10.1016/j.imlet.2017.06.011

Cited by 35 publications

(24 citation statements)

References 41 publications

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“…Sodium bisulfite modification was performed using the EZ DNA Methylation‐Gold™ Kit (Zymo Research, Orange, CA, USA). DNA methylation was measured by quantitative methylation‐specific polymerase chain reaction (PCR) (qMSP), using a LightCycler 480 machine (Roche Diagnostics, Mannheim, Germany), as described in previous studies . The qMSP primer sequences for SHMT1 are shown in Table .…”

Section: Methodsmentioning

confidence: 99%

“…DNA methylation was measured by quantitative methylation-specific polymerase chain reaction (PCR) (qMSP), using a LightCycler 480 machine (Roche Diagnostics, Mannheim, Germany), as described in previous studies. 16,17 The qMSP primer sequences for SHMT1 are shown in Table 1. The reaction conditions were as follows: initial denaturation at 95°C for 60 seconds, followed by 45 cycles of denaturation at 95°C for 20 seconds, annealing at 58°C for 45 seconds, and extension at 72°C for 20 seconds.…”

Section: Sodium Bisulfite Modification Was Performed Using the Ez Dnamentioning

confidence: 99%

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Serine hydroxymethyltransferase 1 promoter hypermethylation increases the risk of essential hypertension

Wang

Ying

et al. 2018

Clinical Laboratory Analysis

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Background Serine hydroxymethyltransferase 1 (SHMT1) is an enzyme involved in folic acid metabolism and is known to contribute to the development of hypertension. We evaluated the relationship between SHMT1 promoter methylation and essential hypertension (EH). Methods Quantitative methylation‐specific polymerase chain reaction was used to measure the SHMT1 promoter methylation level in 241 EH patients and 288 age‐ and gender‐matched healthy individuals. The diagnostic value of SHMT1 promoter hypermethylation was analyzed using a receiver operating characteristic (ROC) curve. The Gene Expression Omnibus (GEO) database and dual‐luciferase reporter assay were used to validate our findings. Results Compared with the control group, significant differences in SHMT1 promoter methylation were found in both EH and hyperhomocysteinemia groups (P < 0.001 and P = 0.029, respectively). The area under the curve of the diagnosis of SHMT1 promoter hypermethylation for EH was 0.808, with a sensitivity and specificity of 73.9% and 77.8%, respectively. The risk of SHMT1 promoter hypermethylation was significantly higher in the >65‐year group than in the ≤65‐year group (odds ratio = 3.925; 95% confidence interval = 2.141‐7.196). In addition, GEO database analysis showed that 5‐aza‐deoxycytidine increased gene expression in several carotid endothelial cell lines. A dual‐luciferase reporter assay revealed that the target sequence in the SHMT1 promoter upregulated gene expression. Conclusion Our findings indicate that SHMT1 promoter hypermethylation increases the risk of EH and may be a promising biomarker for EH.

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Section: Methodsmentioning

confidence: 99%

Section: Sodium Bisulfite Modification Was Performed Using the Ez Dnamentioning

confidence: 99%

Serine hydroxymethyltransferase 1 promoter hypermethylation increases the risk of essential hypertension

Wang

Ying

et al. 2018

Clinical Laboratory Analysis

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“…Quantitative methylation-specific polymerase chain reaction (qMSP) was used to detect the methylation levels. qMSP was performed as described in our previous studies (20)(21)(22). The percent of methylated reference (PMR) was used to represent gene methylation (23,24).…”

Section: Methodsmentioning

confidence: 99%

Significant association of EED promoter hypomethylation with colorectal cancer

et al. 2019

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Colorectal cancer (CRC) is one of the most common and serious types of malignancy worldwide. The embryonic ectoderm development (EED) gene is important to maintain transcriptional repressive states of genes over successive cell generations. The present study aimed to investigate the association between EED methylation and CRC. A total of 111 CRC tissue samples, 111 paired para-tumor tissues and 20 colorectal normal tissues were obtained for EED methylation assay, which was performed using a quantitative methylation-specific polymerase chain reaction. The percentage of methylated reference was calculated to represent the DNA methylation level. A dual-luciferase reporter gene assay was used to detect the gene promoter activity of a EED fragment. The current results revealed a significant difference in the EED methylation levels among tumor, para-tumor and normal colorectal tissues (tumor vs. para-tumor vs. normal, 5.03±4.61 vs. 8.65±11.50 vs. 40.12±45.31; F=45.014; P<0.0001). The dual-luciferase reporter gene assay demonstrated that the transcriptional activity of recombinant pGL3-EED plasmid was significantly higher compared with that of the pGL3-Basic control vector (fold-change, 3.15; P=0.014), which suggests the EED fragment can promote gene expression. In conclusion, the present study demonstrated that EED hypomethylation may be an important factor associated with CRC.

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“…The details of DNA extraction from tissue samples and bisulfite conversion were as previously described . The qMSP was applied to measure the methylation level, and the details of qMSP were as shown in our previous publications . The detail of PCR was conducted as before and the qMSP primer sequences were shown in Table .…”

Section: Methodsmentioning

confidence: 99%

“…DNA methylation is one of the widely studied epigenetic mechanisms, and it often occurs in CpG dinucleotide‐rich regions. Aberrant gene methylation was deemed as one of the most promising diagnostic tools for cancer, including CRC …”

Section: Introductionmentioning

confidence: 99%